HPLC for Pharmaceutical Scientists 2007 (Part 2)

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HPLC for Pharmaceutical Scientists 2007 (Part 2)

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The process of analyte retention in high-performance liquid chromatography (HPLC) involves many different aspects of molecular behavior and interactions in condensed media in a dynamic interfacial system. Molecular diffusion in the eluent flow with complex flow dynamics in a bimodal porous space is only one of many complex processes responsible for broadening of the chromatographic zone. Dynamic transfer of the analyte molecules between mobile phase and adsorbent surface in the presence of secondary equilibria effects is also only part of the processes responsible for the analyte retention on the column. These processes just outline a complex picture that...

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