HPLC for Pharmaceutical Scientists 2007 (Part 8B)

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HPLC for Pharmaceutical Scientists 2007 (Part 8B)

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Choice of Stationary Phase Ideally for a reversed-phase separations, the retention factors (k) for all com- ponents in a sample should lie between 1 and 10 to achieve separation in a reasonable time. For a given stationary phase the k of a particular component can be controlled by changing the solvent composition of the mobile phase. However, the impact of eluent composition will depend on the type of sta- tionary phase and the nature of the components in the mixture.

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