Choice of Stationary Phase
Ideally for a reversed-phase separations, the retention factors (k) for all com-
ponents in a sample should lie between 1 and 10 to achieve separation in a
reasonable time. For a given stationary phase the k of a particular component
can be controlled by changing the solvent composition of the mobile phase.
However, the impact of eluent composition will depend on the type of sta-
tionary phase and the nature of the components in the mixture.