HPLC for Pharmaceutical Scientists 2007 (Part 8C)

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HPLC for Pharmaceutical Scientists 2007 (Part 8C)

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Some typical equilibration times for various column dimensions are shown in Table 8-7; however, these should only be used as a guide. If complete equi- libration is not achieved, early eluting components may show differences in retention from run to run. An experiment could be run such that three dif- ferent methods could be run with different equilibration times.

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