HPLC for Pharmaceutical Scientists 2007 (Part 8D)

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HPLC for Pharmaceutical Scientists 2007 (Part 8D)

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Concluding Remarks. Extreme changes in selectivity and reversal of elution order of phenolic isomeric compounds were obtained after changing either the pH of the mobile phase, the temperature of the system, or the type of organic eluent employed. Changes in the analyte ionization state were observed upon increasing the acetonitrile composition as well as the temperature. Method development for ionizable analytes requires a judicious choice of the mobile-phase conditions and system parameters in order to perform the analysis of the compounds in their desired ionization state. ...

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