This book is intended as a companion to Thermodynamics and Kinetics for the
Biological Sciences, published in 2000.These two books are based on a course
that has been given to ﬁrst-year graduate students in the biological sciences
at Duke University.These students typically do not have a strong background
in mathematics and have not taken a course in physical chemistry. The
intent of both volumes is to introduce the concepts of physical chemistry that
are of particular interest to biologists with a minimum of mathematics.
Neuronal PAS domain protein 2 (NPAS2) is a circadian rhythm-associated
transcription factor with two heme-binding sites on two PAS domains. In
the present study, we compared the optical absorption spectra, resonance
Raman spectra, heme-binding kinetics and DNA-binding characteristics of
the isolated fragment containing the N-terminal basic helix–loop–helix
(bHLH) of the first PAS (PAS-A) domain of NPAS2 with those of the
PAS-A domain alone.
Laser-induced thermal explosion of metallic nanoparticles originates from nonlinear optical effect. Nonlinear optical properties of nanoparticle materials depend strongly on their size and shape. Several methods were proposed to produce nanoparticles of a controlled size and welldefined distribution. We studied to prepare silver nanoparticles by laser ablation. The characteristic spectral feature of the silver nanoparticles (peak around 400nm) was found in the absorption spectra measured by a UV-Vis 2450 spectrometer. ...
Deviations from Beer’s law (i.e. deviations from the
linearity of absorbance vs. concentration):
– Intermolecular interactions at higher concentrations
– Chemical reactions (species having different spectra)
– Peak width/polychromatic radiation
Beer’s law is only strictly valid with single-frequency radiation
Not significant if the bandwidth of the monochromator is less
than 1/10 of the half-width of the absorption peak at halfheight.
Owing to theuseof porphyrins inphotodynamic therapy for
the treatment of malignant tumors, and the preferential
interaction of lectins with tumor cells, studies on lectin–
porphyrin interactionareof significant interest. In this study,
the interaction of several free-base and metalloporphyrins
withMomordica charantia(bitter gourd) lectin (MCL) was
investigated by absorption spectroscopy. Difference absorp-tion spectra revealed that significant changes occur in the
Soret band region of the porphyrins on binding to MCL....
Chapter 14 introduce to ethers, epoxides, and sulfides. After completing this chapter, students will be able to: Draw and name ethers and heterocyclic ethers, including epoxides; explain the trends in their boiling points, solubilities, and solvent properties; determine the structures of ethers from their spectra, and explain their characteristic absorptions and fragmentations;...
On the basis of amino acid sequences and crystal structures
of similar enzymes, it is proposed that Met95 of the heme-regulatedphosphodiesterase fromEscherichiacoli(EcDOS)
acts as aheme axial ligand. Inaccordancewith this proposal,
the Soret and visible optical absorption and magnetic
circular dichroism spectra of the Fe(II) complexes of the
Met95Ala and Met95Leu mutant proteins indicate that
these complexes are five-coordinated high-spin, suggesting
that Met95 is an axial ligand for the Fe(II) complex. ...