It would have been much easier for me to write this
ACKNOWLEDGEMENT if I were a well established scientist of
international fame. I could then write in a pastoral manner about sweet
recollections of the past, starting with a certain scientist, also internationally
famous of course, who came to visit my lab and suggested that I should write
such a book. Knowing that the whole world was watching and waiting, I had
set aside all the other very important works and devoted most of my time to
the writing of this path-blazing masterpiece.
This method is real-time PCR, which can be a qualitative or quantitative assay since
amplification and detection of amplified products occur simultaneously. Then, typing is
performed. Typing is primarily used for epidemiologic investigations, for studies on
pathogenesis such as multiple serotype infections, for unusual or especially severe
infections, or for treatment approaches such as high titer γ-globulin. The nucleotide
sequences from these fragments were determined by a DNA auto sequencer with
fluorescent dideoxy chain terminators.
GTP-binding proteins of the Rab family were cloned from
human platelets using RT-PCR. Clones corresponding to
twonovelRabproteins,Rab31 andRab32, and toRab11A,
which had not been detected in platelets previously, were
isolated. The coding sequence of Rab31(GenBank acces-sion no. U59877) corresponded to a 194 amino-acid protein
of 21.6 kDa. TheRab32sequence was extended to 1000
nucleotides including 630 nucleotides of coding sequence
(GenBank accession no.