Fatty acid synthase (FAS) is uniquely expressed at high levels in cancer
cells and adipose tissue. The objectives of this study were to identify, purify
and validate soy FAS inhibitory peptides and to predict their binding
modes. Soy peptides were isolated from hydrolysates of purifiedb-conglyci-nin by co-immunoprecipitation and identified using LC-MS⁄MS.
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis
GSK837149A has been identified as a selective inhibitor of human fatty
acid synthase (FAS). The compound was first isolated as a minor impurity
in a sample found to be active against the enzyme in a high-throughput
Ở các sinh vật ái khí, pyruvat (tạo thành từ đường phân) bị oxy hóa thành CO2 and acetyl-CoA với sự tham gia của coenzyme A. Sự oxy hóa acetyl –CoA tiếp theo bằng một loạt các phản ứng thuộc chu trình acid citric.
Fatty acids are an important energy source, for they yield over twice as much energy as an equal mass of carbohydrate or protein. In humans, the primary dietary source of fatty acids is triacylglycerols. This lecture will describe the metabolism of fatty acids. The two main components of fatty acid metabolism are β oxidation and fatty acid synthesis. Upon completion of this lecture, you will understand that the fatty-acid breakdown reactions of β oxidation result in the formation of reduced cofactors and acetyl-CoA molecules, which can be further catabolized to release free energy.
The pentacyclic triterpenes, in particular ursolic acid and oleanolic acid
and their derivatives, exist abundantly in the plant kingdom, where they
are well known for their anti-inflammatory, antitumour and antimicrobial
Escherichia colifatty acid cyclopropane synthase (CFAS)
was overproduced and purified as a His6
This recombinant enzyme is as active as the native enzyme
with aKmof 90lMforS-AdoMet and a specific activity of
. The enzyme is devoid of organic
or metal cofactors and is unable to catalyze the wash-out of
themethyl protons ofS-AdoMet to the solvent, data that do
not support the ylidemechanism. Inactivationof the enzyme
by 5,5¢-dithiobis-(2-nitrobenzoic acid) (DTNB), a pseudo
first-order process with a rate constant of 1.
Although metabolites and energy balance have long been known to play roles in the regulation of food intake, the potential role of fatty
acid metabolism in this process has been considered only recently. Fatty acid synthase (FAS) catalyzes the condensation of acetyl-CoA and
malonyl-CoA to generate long-chain fatty acids in the cytoplasm, while the breakdown of fatty acids (h-oxidation) occurs in mitochondria
and is regulated by carnitine palmitoyltransferase-1 (CPT-1), the rate-limiting step for the entry of fatty acids into the mitochondria....
The amino acid residue tryptophan 27 of 6,7-dimethyl-8-ribityllumazine synthase of the yeastSchizosaccharomyces
pombewas replacedby tyrosine. The structures of theW27Y
mutant protein in complex with riboflavin, the substrate
analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidin-edione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine,weredeterminedbyX-raycrystallography
at resolutions of 2.7–2.8 A
ent-Kaurene is a tetracyclic diterpene hydrocarbon and a biosynthetic
intermediate of the plant hormone gibberellins. In flowering plants, ent-kaurene is biosynthesized from geranylgeranyl diphosphate (GGDP) by two
distinct cyclases,ent-copalyl diphosphate synthase (CPS) and ent-kaurene
The fruit of tomato (Solanum lycopersicumL.) is a berry: red, fleshy and
rich in seeds. Its colour is due to the high content of lycopene whose syn-thesis is activated by the phytoene synthase 1 (PSY1) enzyme, encoded by
Psy1which is distinct fromPsy2.
We describe a strategy for systematic amplification of chitin
synthase genes (chs) in the filamentous ascomycetes plant-pathogenBotrytis cinereausing PCR with multiple degen-erate primers designed on specific and conserved sequence
motifs. Eight distinctchsgeneswere isolated, namedBcchs I,
II, IIIa, IIIb, IV, V, VIandVII. They probably constitute
the entire chsmultigenic family of this fungus, as revealed
by careful analysis of six euascomycetes genomes.
By replacing speciﬁc amino acids at positions 112, 147 and 152 of the human aldosterone synthase (CYP11B2) with the corresponding residues from human, mouse or rat 11b-hydroxylase (CYP11B1), we have been able to investigate whether these residues belong to structural determinants of individual enzymatic activities. When incubated with 11-deoxycorticosterone (DOC), the 11b-hydroxylation activity of the mutants was most eﬀectively increased by combining D147E and I112P (sixfold increase).
Liver metabolism is inﬂuenced by hormones and nutrients. Amino acids such as glutamine or leucine induce an anabolic response, which resembles that of insulin in muscle and adipose tissue. In this work, the signalling pathways and the eﬀects of insulin were compared to those of glutamine and leucine in isolated hepatocytes from normal and streptozotocin-diabetic rats.
This study was aimed at investigating the physiological role of ferredoxin-glutamate synthases (EC 188.8.131.52), NADH-glutamate synthase (EC 184.108.40.206)
and carbamoylphosphate synthetase (EC 220.127.116.11) in Arabidopsis. Pheno-typic analysis revealed a high level of photorespiratory ammonium, gluta-mine⁄glutamate and asparagine⁄aspartate in theGLU1mutant lacking the
major ferredoxin-glutamate synthase, indicating that excess photorespiratory
ammonium was detoxified into amino acids for transport out of the veins....
Acetohydroxyacid synthase (AHAS; EC 18.104.22.168) is the first enzyme in the
biosynthetic pathway of the branched-chain amino acids. It catalyzes the
conversion of two molecules of pyruvate into 2-acetolactate or one mole-cule of pyruvate and one molecule of 2-ketobutyrate into 2-aceto-2-hydrox-ybutyrate.
Using a degenerate primer designed from triterpene synthase sequences, we
have isolated a new gene from the medicinal plantArtemisia annua. The
predicted protein is highly similar to b-amyrin synthases (EC 5.4.99.–),
sharing amino acid sequence identities of up to 86%.
We investigated the role of glutamine synthetases (cytosolic GS1 and chlo-roplast GS2) and glutamate synthases (ferredoxin-GOGAT and NADH-GOGAT) in the inorganic nitrogen assimilation and reassimilation into
amino acids between bundle sheath cells and mesophyll cells for the remo-bilization of amino acids during the early phase of grain filling inZea mays
The product chain length determination mechanism of type II geranyl-geranyl diphosphate synthase from the bacterium, Pantoea ananatis, was
studied. In most types of short-chain (all-E) prenyl diphosphate synthases,
bulky amino acids at the fourth and/or fifth positions upstream from the
first aspartate-rich motif play a primary role in the product determination
A cDNA encoding a novel plant type III polyketide synthase was cloned
and sequenced from the Chinese club moss Huperzia serrata(Huperzia-ceae). The deduced amino acid sequence ofHu. serratapolyketide synthase
1 showed 44–66% identity to those of other chalcone synthase superfamily
enzymes of plant origin.