Xem 1-20 trên 134 kết quả Analysis of columns
  • The Arabidopsis FAE1 b-ketoacyl-CoA synthase (FAE1 KCS) catalyzes the condensation of malonyl-CoA with longchain acyl-CoAs. Sequence analysis of FAE1 KCS predicted that this condensing enzyme is anchored to a membrane by two adjacent N-terminal membrane-spanning domains. In order to characterize the FAE1 KCS and analyze its mechanism, FAE1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharomyces cerevisiae. The membrane-bound enzyme was then solubilized and purified to near homogeneity on a metal affinity column.

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  • This book is intended to supply engineering professionals and students with a comprehensive and definitive reference to statics and dynamics of solids and structures. The book is for use as a resource and design tool in research and development, and for use as a study guide and learning aid in engineering education. The book is written to meet the needs for interactive computing in technical referencing and engineering education.

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  • Introduction: Alcohol usually means ethanol/ethyl alcohol. It has a long history for the human being, and gives euphoric effects and sometimes improves the human relationship. In contrast, there are many cases of violence, injuries, homicides and traffic accidents with drinking; close relation can be observed between ethanol and crimes/accidents. When forensic autopsies are performed, ethanol concentrations in blood and urine are routinely measured. GC analysis of ethanol using the conventional packed columns is described in many of literature [1, 2].

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  • ECONOMIC DEVELOPMENT IN GREENLAND: A TIME SERIES ANALYSIS OF DEPENDENCY GROWTH , AND INSTABILITY Column A presents the coefficient from a regression of school average scores on an index of student quality, pooling all metropolitan schools in the NELS sample 44 and including a fixed effect for each MSA.50 As in the SAT data, peer effects and effectiveness sorting are together substantial, inflating the school-level background index coefficient by 90 percent relative to the coefficient of a within-school regression of individual scores on own characteristics....

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  • ANALYSIS OF CREDIT RATING EQUIT” INDEXES: VOLATILITY COMPARISONS A OPTION CALIBRATION The positive correlation between choice and performance seems to result entirely from the omission of students’ background characteristics; when they are included in Column C, the coefficient becomes negative and significant.

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  • A new automated procedure for preconcentration and analysis of anionic surfactants in water using solid-phase extraction (SPE) in a flow injection (Fl) system together with an UV-vis detection is reported in this paper. The method involves extraction of blue coloured ion-pair complex of anionic surfactants and methylene blue into solid phase and their elution from the SPE column with organic solvent (chloroform stabilised with methanol) and subsequent measurement in an UV-vis flow cell at 650 nm. The system is well optimised before to apply for analysis of real samples.

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  • Chicken pancreatic phospholipase A2 (ChPLA2 ) was purified from delipi-dated pancreases using ammonium sulfate and ethanol precipitation, followed by sequential column chromatography steps on MonoQ Sepha-rose and size exclusion HPLC columns. ChPLA2 was found to be a non-glycosylated monomeric protein with a molecular mass of 14 kDa and a specific activity of 400 UÆmg )1 in the presence of 1 mmsodium taur-odeoxycholate and 4 mmCaCl2with phosphatidylcholine as substrate.

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  • A phospholipase B (PLB) precursor was purified from normal human gran-ulocytes using Sephadex G-75, Mono-S cation-exchange and hydroxyapa-tite columns. The molecular mass of the protein was estimated to be 130 kDa by gel filtration and 22 and 42 kDa by SDS⁄PAGE.

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  • This paper deals with the purification and the partial char-acterization of glutathione S-transferase (GST) isoforms from the clam Ruditapes decussatus. For the first step of purification, two affinity columns, reduced glutathione (GSH)–agarose and S-hexyl GSH–agarose, were mounted in series. Four affinity fractionswere thus recovered. Further purification was performed using anion exchange chroma-tography. Seven fractions,whichpresent aGSTactivitywith 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, were collected and analyzed by RP-HPLC....

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  • Introduction: Small amount of drugs and poisons incorporated into human bodies are hidden among large amounts of biological components, such as proteins, lipids, nucleic acids and membranes. It is not easy to detect only a target compound from such complicated matrices. Before instrumental analysis, extraction procedure is usually essential and very important.

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  • MODERN PRACTICE OF GAS CHROMATOGRAPHY .MODERN PRACTICE OF GAS CHROMATOGRAPHY FOURTH EDITION Edited by Robert L. Grob, Ph.D. Professor Emeritus, Analytical Chemistry, Villanova University Eugene F. Barry, Ph.D. Professor of Chemistry, University of Massachusetts Lowell A JOHN WILEY & SONS, INC. PUBLICATION .Copyright  2004 by John Wiley & Sons, Inc. All rights reserved. Published by John Wiley & Sons, Inc., Hoboken, New Jersey. Published simultaneously in Canada.

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  • Seismic Design of Reinforced Concrete Bridges 38.1 Introduction Two-Level Performance-Based Design • Elastic vs. Ductile Design • Capacity Design Approach 38 38.2 Typical Column Performance Characteristics of Column Performance • Experimentally Observed Performance 38.3 Flexural Design of Columns Earthquake Load • Fundamental Design Equation • Design Flexural Strength • Moment–Curvature Analysis • Transverse Reinforcement Design 38.4 Shear Design of Columns Fundamental Design Equation • Current Code Shear Strength Equation • Refined Shear Strength Equations 38.

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  • .Source: STANDARD HANDBOOK OF ENGINEERING CALCULATIONS SECTION 1 CIVIL ENGINEERING PART 1: STRUCTURAL STEEL DESIGN STEEL BEAMS AND PLATE GIRDERS Most Economic Section for a Beam with a Continuous Lateral Support under a Uniform Load Most Economic Section for a Beam with Intermittent Lateral Support under Uniform Load Design of a Beam with Reduced Allowable Stress Design of a Cover-Plated Beam Design of a Continuous Beam Shearing Stress in a Beam—Exact Method Shearing Stress in a Beam—Approximate Method Moment Capacity of a Welded Plate Girder Analysis of a Riveted Plate Girder Design...

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  • Benefits Analysis Sheet Use this to analyze various benefits packages and quotes from providers you are considering. Type provider names in the {Provider} placeholders at the top of each column and enter itemized information to compare packages at a glance. Health HEALTH PLAN Employer Cost: Employee Cost: Covered: {Provider 1} {Provider 2} {Provider 3} {Provider 4} Not Covered: Additional Information: Dental DENTAL PLAN Employer Cost: Employee Cost: Covered: {Provider 1} {Provider 2} {Provider 3} {Provider 4} Not Covered: Additional Information: Provided By HR.

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  • Chromatography is the separation method for biological molecules from the analytical to the preparative scale. Few of the recent advances in life science, whether in "proteomics" or in the industrial production of recombinant therapeutics, would have been possible without the help of chromatographic separations. Concomitantly, chromatography nowadays stands for much more than a batch column packed with porous particles

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  • This book is characterized by three important features. The authors represent an impressive collection of international workers from Brazil, China, Egypt, Poland, Turkey, and the United States. The majority of the chapters reflect the importance of collaborative efforts in contemporary research. Finally, some chapters are especially useful because of the experimental details that are provided. And it is to be hoped that readers will find that the chapters are both informative and inspirational.

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  • Nongovernmental organizations, though, reach more borrowers in total. The third column shows that nongovernmental organizations can claim about one-half of the 18 million customers in this data set, with banks claiming one-quarter. Donors at large aid agencies have pushed hard to encourage the commercialization of microfinance, but the evidence here suggests that nonprofit microfinance agencies still matter in a big way.

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  • As a next step, we investigate whether the emission elasticity of the population variable changes with population size. Our second model, for which results are reported in column II, therefore allows for a non-linear relationship between population and pollution emissions by including population squared (POP2 ). The linear term becomes statistically insignifi- cant, which suggests that the relationship is not quadratic. 4 We therefore only include the linear population term in the estimations that follow.

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  • From this table of the functional groups one recognizes that any transformation between columns must involve a redox process whereas transformations between rows can be accomplished by acid/base processes. It forms a scaffolding on to which we can hang our knowledge of reactivity, and at the same time it prepares us to think of yet unseen transformations on the basis of how those transformations might be effected.

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  • .CHAPTER THREE CONTROL STATEMENTS 3.1 FOR LOOPS “FOR” loops allow a statement or group of statements to be repeated a fixed number of times. The general form of a for loop is for index = expression statement group X end The expression is a matrix and the statement group X is repeated as many times as the number of elements in the columns of the expression matrix. The index takes on the elemental values in the matrix expression. Usually, the expression is something like m:n or m:i:n where m is the beginning value, n the ending value, and i is the...

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