Now an established handbook of principles and techniques, this text develops an understanding of the relevance of four fundamental properties of the analyte: shape, polarity, charge and size, to the three key types of analysis: separation, identification and quantification. The third edition of Analytical Biochemistry has now been fully updated in content and format, making it even more accessible to students learning how to select analytical techniques and recognise their scope and limitations.
Laccase-catalyzed polymerization of tyrosine and tyrosine-containing pep-tides was studied in the presence and absence of ferulic acid (FA).
Advanced spectroscopic methods such as MALDI-TOF MS, EPR, FTIR
microscopy and HPLC-fluorescence, as well as more conventional analyt-ical tools: oxygen consumption measurements and SDS⁄PAGE were used
in the reaction mechanism studies. Laccase was found to oxidize tyrosine
and tyrosine-containing peptides, with consequent polymerization of the
Transcription factors are DNA-binding proteins that regulate key biologi-cal processes. Their interactions with DNA are commonly analyzed with
gel-based electrophoretic mobility shift assay (EMSA) using radioactively
labeled probes. Within various fields of research, there exists an increasing
demand to develop assays with faster sample throughput combined with
improved sensitivity, increased analytical range, and precise quantification.
Sắc ký lỏng cao áp (High-performance liquid chromatography), đôi khi gọi là High-pressure liquid chromatography_HPLC) là một kỹ thuật sắc ký sử dụng để phân tách một hỗn hợp trong lĩnh vực hóa phân tích (analytical chemistry) và sinh hóa (biochemistry) với mục đích xác định, định lượng và tinh khiết từng thành phân riêng lẻ của hợp chất. HPLC cũng được xem là một kỹ thuật đo đạc trong hóa phân tích, thay vì kỹ thuật trọng lượng (gravitimetric technique).
THERE IS probably no single course in “laboratory safety or chemical safety” at your college or
university. Why not? Chemistry curricula have developed over many decades with a focus on the main
topics of chemistry: organic, inorganic, physical chemistry, analytical chemistry, and (more recently)
biochemistry. For decades, the topic of chemical safety was included at the margins of lab courses,
mostly taught in a small way as a footnote to various lab experiments and procedures. Some chemists and
chemistry teachers were aware of the importance of safety, while many were not.