The protein arginine methyltransferase (PRMT) family of enzymes cata-lyzes the transfer of methyl groups from S-adenosylmethionine to the gua-nidino nitrogen atom of peptidylarginine to form monomethylarginine or
dimethylarginine. We created several less polar analogs of the specific
PRMT inhibitor arginine methylation inhibitor-1, and one such compound
was found to have improved PRMT inhibitory activity over the parent
RNomics is a newly emerging sub-discipline of genomics that categorically
studies the structure, function and processes of non-coding ribo nucleic acids in
a cell. MicroRNomics is a sub-field of RNomics that describes the biogenesis and
mechanisms of tiny RNA regulators, and their involvement in the processes of
development, differentiation, cell proliferation, cell death, chromosomal segregation
and metabolism. The discovery of microRNAs in species ranging from
Caenorhabditis elegans (C.
Current evidence suggests that the beneficial effects of statins seem
to be mediated not only by their lipid-lowering properties but also
by improving vascular endothelial cell function. Chronic inhibition
of endothelial nitric oxide synthesis by oral administration of
Nw-nitro-L-arginine methyl ester (L-NAME) to rats induces early
vascular inflammation and subsequent arteriosclerosis.
The human 57 kDa Ki-1 antigen (Ki-1⁄57) is a cytoplasmic and nuclear
protein, associated with Ser⁄Thr protein kinase activity, and phosphorylat-ed at the serine and threonine residues upon cellular activation. We have
shown that Ki-1⁄57 interacts with chromo-helicase DNA-binding domain
protein 3 and with the adaptor⁄signaling protein receptor of activated
kinase 1 in the nucleus.
Previous structural andmutagenesis studies indicate that the
invariant a-amino and a-carboxyl groups of glutamate
receptor agonists are engaged in polar interactions with
oppositely charged, conserved arginine and glutamate resi-dues in the ligand-binding domain ofa-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor.To examine
the role of these residues (R507 and E727 in the GluR-D
subunit) in the discrimination between agonists and anta-gonists, we analyzed the ligand-binding properties of
homomeric GluR-D and its soluble ligand-binding domain
withmutations at thesepositions....
The cytoplasmic and nuclear protein Ki-1⁄57 was first identified in malig-nant cells from Hodgkin’s lymphoma. Despite studies showing its phos-phorylation, arginine methylation, and interaction with several regulatory
proteins, the functional role of Ki-1⁄57 in human cells remains to be
Histones are modified post-translationally, e.g. by methylation of lysine
and arginine residues, and by phosphorylation of serine residues. These
modifications regulate processes such as gene expression, DNA repair, and
mitosis and meiosis. Recently, evidence has been provided that histones are
also modified by covalent binding of the vitamin biotin.