Bacterial genetics

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  • Part 1 "Textbook of microbiology" presentation of content: Historical introduction, culture media, bacterial taxonomy, bacterial genetics, culture methods, complement system, immune response, autoimmunity, nonsporing anacrobes... And other content.

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  • Introduction to molecular biology…(…in one hour!!) Stephen Edwards Overview. Overview of the cell Different sizes/functions Organised structure Bacterial genetics are different Eukaryotic cell DNA ontained in the nucleus Arranged in 22.

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  • The metabolic network is an important biological network which consists of enzymes and chemical compounds. However, a large number of meta-bolic pathways remains unknown, and most organism-specific metabolic pathways contain many missing enzymes. We present a novel method to identify the genes coding for missing enzymes using available genomic and chemical information from bacterial genomes.

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  • Chapter 14 - Prokaryotic and organelle genetics. This chapter begins with a discussion of the origins of yeast mitochondrial work. It then discusses the development of Chlamydomonas reinhardtii as a model organism. It also shows how early work on the organelle genetics of Chlamydomonas and yeast were carried out relatively independently; but inevitably the rationales soon paralleled one another.

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  • GE23077, a novel microbial metabolite recently isolated from Actinomadurasp. culture media, is a potent and selective inhibitor of bacterial RNApolymerase (RNAP). It inhibitsGram-positive (Bacillus subtilis) andGram-negative (Escherichia coli)RNAPs with IC50 values (i.e. the concen-tration at which the enzyme activity is inhibited by 50%) in the 10 )8 Mrange, whereas it is not active onE. coliDNA polymerase or on eukaryotic (wheat germ) RNAP II (IC50 values10 )4 Min both cases).

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  • Although the annotation of the complete genome sequence ofMycoplasma pneumoniaedid not reveal a bacterial type I signal peptidase (SPase I) we showed experimentally that such an activity must exist in this bacterium, by determining the N-terminus of the N-terminal gene product P40 of MPN142, formerly called ORF6 gene. Combining mass spectrometry with a method for sulfonating specifically the free amino terminal group of proteins, the cleavage site for a typical signal peptide was located between amino acids 25 and 26 of the P40 precursor protein. ...

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  • Inflammatory bowel disease research is changing. Progress in defining and treating these diseases is advancing in lock step with the furious pace of technological advances that continue to refine the tools of discovery. With sequencing of the entire genome completed, genetics research is providing direction for molecular and immunological in vivo and in vitro investigation, which in turn directs the development of targeted therapeutics.

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  • The pathogenesis of osteomyelitis is a complex process involving interactions between a host and an infectious agent. The host’s inflammatory response to a pathogen can further the physical spread of disease by clearing space in bone. Predisposing genetic differences in immune function are increasingly seen as an aetiological factor in some cases of osteomyelitis. Acquired factors such as diseases causing immune or vascular compromise and implantation of foreign materials are frequently involved in the disease process as well. ...

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  • Take molecular genetics and bioinformatics for example; these are perhaps two of the most exciting areas of biology and are beginning to have an impact on other areas of medical therapeutics such as cancer and diabetes, and provide a signpost to ‘personalised medicine’. Yet recent genome wide association (GWAS) studies of large samples, have demonstrated that in schizophrenia around 1000 or more genetic variants of low penetrance may be implicated in the heritability of schizophrenia.

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  • The complex world of polysaccharides is a compilation of the characteristics of a variety of polysaccharides from plants, animals and microorganisms. The diversity of these polysaccharides arises from the structural variations and the monosaccharide content which is under genetic control. The chemical and physical properties have made them useful in many pharmaceutical, food and industrial applications.

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  • Human growth is a measure of the physiological processes associated with birth weight, genetics, and environment. Poor environmental factors, including inadequate health care and nutrition can prevent the attainment of one’s full growth potential (Martorell, 1999; Pelletier, 1994; Monckeberg 1992). Health care providers that practice high quality prenatal and child healthcare can directly influence the efficacy of the production of child health inasmuch as their practices have an empirical basis.

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  • When students move from the lecture hall to the microbiology laboratory, they need help bridging the gap between the theory and the practice of what they are learning. The equipment is unfamiliar, the procedures are unfamiliar, and many of the materials they are handling are unfamiliar. Linking the information from their classroom lectures to the laboratory procedures is necessary for their ultimate success. Our goal for this laboratory manual is to provide the bridge that helps students integrate their classroom lectures with their laboratory experiences.

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  • Tham khảo sách 'lab exercises in organismal and molecular microbiology_1', y tế - sức khoẻ, y học thường thức phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

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  • The iron responsive regulator Irr is found in a wide range ofa-proteobac-teria, where it regulates many genes in response to the essential but toxic metal iron. Unlike Fur, the transcriptional regulator that is used for iron homeostasis by almost all other bacterial lineages, Irr does not sense Fe 2+ directly, but, rather, interacts with a physiologically important form of iron, namely heme.

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  • The gene encoding a bacterial type 1 RNase H, termed RBD-RNase HI, was cloned from the psychrotrophic bacteriumShewanellasp. SIB1, over-produced inEscherichia coli, and the recombinant protein was purified and biochemically characterized. SIB1 RBD-RNase HI consists of 262 amino acid residues and shows amino acid sequence identities of 26% to SIB1 RNase HI, 17% toE. coliRNase HI, and 32% to human RNase H1.

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  • Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: A genetic epidemiological model to describe resistance to an endemic bacterial disease in livestock: application to footrot in sheep

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  • Cells have evolved complex and overlapping mechanisms to protect their proteins from aggregation. However, several reasons can cause the failure of such defences, among them mutations, stress conditions and high rates of protein synthesis, all common consequences of heterologous protein pro-duction.

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  • Proteins might aggregate into ordered or amorphous structures, utilizing relatively short sequence stretches, usually organized in b-sheet-like assem-blies. Here, we attempt to list all available software, developed during the last decade or so, for the prediction of such aggregation-prone stretches from protein primary structure, without distinguishing whether these algo

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  • Inclusion bodies are insoluble protein aggregates usually found in recombi-nant bacteria when they are forced to produce heterologous protein species. These particles are formed by polypeptides that cross-interact through sterospecific contacts and that are steadily deposited in either the cell’s cytoplasm or the periplasm.

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  • AraL fromBacillus subtilisis a member of the ubiquitous haloalkanoate dehalogenase superfamily. ThearaLgene has been cloned, over-expressed in Escherichia coliand its product purified to homogeneity. The enzyme displays phosphatase activity, which is optimal at neutral pH (7.0) and 65C.

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