Biochemistry samples

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  • The enzymatic hydrolysis of cellulose encounters various limitations that are both substrate- and enzyme-related. Although the crystallinity of pure cellulosic Avicel plays a major role in determining the rate of hydrolysis by cellulases from Trichoderma reesei, we show that it stays constant during enzymatic conversion. The mode of action of cellulases was investigated by studying their kinetics on cellulose samples.

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  • Previous studies have suggested that microRNAs (miRNAs) may play important roles in tumorigenesis, but little is known about the functions of most miRNAs in cancer development. In the present study, we set up a cell-based screen using a luciferase reporter plasmid carrying the whole 4.7 kb 3¢-UTR of estrogen receptor a(ERa) mRNA cotransfected with a synthetic miRNA expression library to identify potential ERa-targeting miRNAs.

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  • Constitutively expressed human cytochrome P450 2D6 (CYP2D6; EC 1.14.14.1) is responsible for the metabolism of approximately 25% of drugs in common clinical use. It is widely accepted that CYP2D6 is localized in the endoplasmic reticulum of cells; however, we have identified this enzyme in the mitochondria of human liver samples and found that extensive inter-individual variability exists with respect to the level of the mitochondrial enzyme.

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  • Blackwell Publishing. Book Condition: New. pp. 384 Veterinary Laboratory Medicine covers all aspects of basic clinical biochemistry and haematology, and includes test-by-test interpretation of laboratory results. Information is provided on sampling techniques, the selection and use of an external laboratory, as well as near-patient testing and the practice laboratory.

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  • To better understand the mechanism underlying colorectal carcinoma (CRC) genesis or metastasis, and to search for potential markers for CRC prognosis, a comparative proteomic analysis was performed on CRC tissue. Proteins were extracted from normal colorectal mucosa, non-metastatic CRC (nmCRC) and metastatic CRC (mCRC) tissue samples.

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  • Transcription factors are DNA-binding proteins that regulate key biologi-cal processes. Their interactions with DNA are commonly analyzed with gel-based electrophoretic mobility shift assay (EMSA) using radioactively labeled probes. Within various fields of research, there exists an increasing demand to develop assays with faster sample throughput combined with improved sensitivity, increased analytical range, and precise quantification.

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  • Genomicfattyacid elongation 1 (FAE1) clones from high erucic acid (HEA)Brassica napus, Brassica rapaandBras-sicaoleracea, and lowerucicacid (LEA)B. napuscv.Westar, were amplifiedbyPCRand expressed inyeast cells under the control of the strong galactose-inducible promoter. As expected, yeast cells expressing theFAE1genes from HEA Brassicaspp. synthesized very long chain monounsaturated fatty acids that are not normally found in yeast, while fatty acid profiles of yeast cells expressing theFAE1gene from LEA B. napuswere identical to control yeast samples....

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  • International Tables for Crystallography, Volume F, Crystal- lography of Biological Macromolecules, was commissioned by the International Union of Crystallography (IUCr) in recognition of the extraordinary contributions that knowledge of macro- molecular structure has made, and will make, to the analysis of biological systems, from enzyme catalysis to the workings of a whole cell.

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  • Diagnosis is fundamental to the appropriate treatment and wellbeing of the equine patient. Despite the many excellent clinical texts that are available, few seem to explain in sufficiently precise terms which clinicopathological tests are appropriate or how particular techniques should be performed. The first edition of this book was designed to provide an illustrated practical guide to the various diagnostic techniques employed in equine medicine. This second edition is an update by international experts in the field.

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  • Tham khảo sách 'diagnostic techniques in equine medicine', y tế - sức khoẻ, y học thường thức phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

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