Transglutaminase (TGase) catalyses the post-translational modiﬁcation of proteins by transamidation of available glutamine residues. While several TGase genes of ﬁsh and arthropods have been cloned and appear to have similar structures to those of mammals, no homologous gene has been found in lower eukaryotes. We have cloned the acellular slime mold Physarum polycephalum TGase cDNA using RT-PCR with degenerated primers, based on the partial amino acid sequence of the puriﬁed enzyme. The cDNA contained a 2565-bp ORF encoding a 855-residue polypeptide.
The risk management processes described in this book had their genesis well over 20 years ago when I accepted a position at the University of Southampton. There I met and worked with Dr Chris Chapman, already an acknowledged expert in project risk, with an established relationship with BP and an extensive client base in Canada. Chris involved me in his consulting activities in North America, primarily associated with quantitative risk analyses of large projects in the hydroelectric and the oil and gas industries....
This monograph (a preferable term here to ‘book’, I believe) was conceived after I had done a good deal of broadcasting, within the UK and internationally, on the Gulf Coast oil spill. Time is always limited in a broadcast, and facts and valid perspectives need to be got across succinctly to the exclusion of shallow comments which hardly leave a viewer or listener any better informed. I like to go to a broadcast having made a few jottings from news sources which as well as being possible material for the broadcast have attuned my mind to the topic shortly before...
Linear IgA Disease Linear IgA disease, once considered a variant form of dermatitis herpetiformis, is actually a separate and distinct entity. Clinically, these patients may resemble individuals with DH, BP, or other subepidermal blistering diseases. Lesions typically consist of papulovesicles, bullae, and/or urticarial plaques predominantly on central or flexural sites. Oral mucosal involvement occurs in some patients. Severe pruritus resembles that seen in patients with DH.
The number of DNA sequences and transcription factors that regulate transcription is much greater than originally anticipated. Most genes contain at least 15–20 discrete regulatory elements within 300 bp of the transcription start site. This densely packed promoter region often contains binding sites for ubiquitous transcription factors such as CAAT box/enhancer binding protein (C/EBP), cyclic AMP response element–binding (CREB) protein, selective promoter factor 1 (Sp-1), or activator protein 1 (AP-1).
The intestinal fatty acid-binding protein (I-FABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in uptake of dietary fatty acids and their intracellular transport. We have determined the sequence of the gene encoding I-FABP in zebrafish. The zebrafish I-FABP gene contains four exons interrupted by three introns. Radiation hybrid mapping assigned the I-FABP gene to linkage group 1. A 924 bp sequence 5Vupstream of the initiation codon in the I-FABP gene contained several putative cis-acting regulatory elements.
The rhesus (Rh) blood group antigens are of considerable importance in
transfusion medicine as well as in newborn or autoimmune hemolytic
diseases due to their high antigenicity. We identified a major DNaseI
hypersensitive site at the 5¢ flanking regions of bothRHDandRHCEexon
1. A 34 bp fragment located at)191 to)158 from a translation start posi-tion, and containing the TCCCCTCCC sequence, was involved in enhan-cing promoter activity, which was assessed by luciferase reporter gene
Thebrain fattyacid-bindingprotein (B-FABP) is involved in
brain development and adult neurogenesis. We have deter-mined the sequence of the gene encoding the B-FABP in
zebrafish. The zebrafish B-FABP gene spans 2370 bp and
contains four exons interruptedby three introns. The coding
sequence of zebrafishB-FABP gene is identical to its cDNA
sequence and the coding capacity of each exon is the same as
that for the human and mouse B-FABP genes. A 1249 bp
sequence 5¢ upstream of exon 1 of the zebrafish B-FABP
gene was cloned and sequenced. ...