Using interferometry-based biosensors the binding and release of endothe-lial and neuronal nitric oxide synthase (eNOS and nNOS) from calmodulin (CaM) was measured. In both isoforms, binding to CaM is diffusion lim-ited and within approximately three orders of magnitude of the Smolu-chowski limit imposed by orientation-independent collisions.
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Genome-wide identification and analyses of the rice calmodulin and related potential calcium sensor proteins
Tuyển tập các báo cáo nghiên cứu khoa học ngành toán học được đăng trên tạp chí toán học quấc tế đề tài: A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3', 5'-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3’, 5’-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium
Humanether a` go-go potassium channels (hEAG1) open in response to
membrane depolarization and they are inhibited by Ca
(CaM), presumably binding to the C-terminal domain of the channel sub-units. Deletion of the cytosolic N-terminal domain resulted in complete
abolition of Ca
⁄CaM sensitivity suggesting the existence of further CaM
Calmodulin (CaM) is a cytosolic Ca
signal-transducing protein that
binds and activates many different cellular enzymes with physiological rele-vance, including the nitric oxide synthase (NOS) isozymes. CaM consists of
two globular domains joined by a central linker; each domain contains an
EF hand pair.
Calmodulin (CaM) is phosphorylated in vitro and in vivo by multiple protein-serine/threonine and protein-tyrosine kinases. Casein kinase II and myosin light-chain kinase are two of the well established protein-serine/threonine kinases implicated in this process. On the other hand, within the protein-tyrosine kinases involved in the phosphorylation of CaM are receptors with tyrosine kinase activity, such as the insulin receptor and the epidermal growth factor receptor, and nonreceptor protein-tyrosine kinases, such as several members of the Src family kinases, Janus kinase 2, and p38Syk....
We show that epigallocatechin-3 gallate (EGCG), a major
component of green tea, stimulates phospholipase D (PLD)
activity in U87 human astroglioma cells. EGCG-induced
PLD activation was abolished by the phospholipase C
(PLC) inhibitor and a lipase inactive PLC-c1mutant, which
is dependent on intracellular or extracellular Ca
possible involvement of Ca
/calmodulin-dependent pro-teinkinase II (CaMkinase II).
The interaction of the adenylate cyclase catalytic domain
(AC) of the Bordetella pertussismajor exotoxin with its
activator calmodulin (CaM) was studied by time-resolved
fluorescence spectroscopy using three fluorescent groups
located indifferent regions ofAC: tryptophan residues (W69
and W242), a nucleotide analogue (3¢-anthraniloyl-2¢-deoxyadenosine 5¢-triphosphate, Ant-dATP) and a cysteine-specific probe (acrylodan).
Tham khảo luận văn - đề án 'báo cáo khoa học: calmodulin binds to maize lipid transfer protein and modulates its lipids binding ability', luận văn - báo cáo, báo cáo khoa học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả
The light chain binding domain of rat myosin 1d consists of two IQ-motifs,
both of which bind the light chain calmodulin (CaM). To analyze the
Myo1d ATPase activity as a function of the IQ-motifs and Ca2+
binding, we expressed and affinity purified the Myo1d constructs Myo1d-head, Myo1d-IQ1, Myo1d-IQ1.2, Myo1d-IQ2 and Myo1dDLV-IQ2. IQ1
exhibited a high affinity for CaM both in the absence and presence of free
The molecular mechanism of the presynaptic neurotoxicity
of snake venom phospholipases A2(PLA2s) is not yet fully
elucidated. Recently, newhigh-affinity binding proteins for
toxins have been discovered, including the important
sensor, calmodulin (CaM). In the present
study, the mode of interaction of group IIAPLA2swiththe
-bound form of CaM was investigated by mutational
analysis of ammodytoxin A (AtxA) from the long-nosed
viper (Vipera ammodytes ammodytes).
Several crystal andNMRstructures of calmodulin (CaM)in
complex with fragments derived from CaM-regulated pro-teins have been reported recently and reveal novel ways for
CaMto interact with its targets. This review will discuss and
compare features of the interaction between CaM and its
target domains derived from the plasma membrane
-pump, the Ca
-channel, the Ca
CaM-dependent kinase kinase and the anthrax exotoxin.
In human neutrophils, both changes in intracellular Ca
, and activation of phosphatidyl-inositol-3 kinase (PtdIns3K) have been proposed to play a
role in regulating cellular function induced by chemoattr-actants. In this studywe have investigated the role of [Ca
and its effector molecule calmodulin in human neutrophils.
Chimeric calcium/calmodulin dependent protein kinase
(CCaMK) is characterized by the presence of a visinin-like
-binding domain unlike other known calmodulin-dependent kinases. Ca
-Binding to the visinin-like domain
leads to autophosphorylation and changes in the affinity
for calmodulin [Sathyanarayanan P.V., Cremo C.R. &
Poovaiah B.W. (2000)J. Biol. Chem. 275, 30417–30422].
Here, we report that the Ca
-stimulated autophosphory-lation of CCaMK results in time-dependent loss of enzyme
Peptide tương tự cũng đã được tổng hợp với một nắp acetyl Hcontaining lysine N-thiết bị đầu cuối để truyền đạt một radiolabel peptide và các sản phẩm photolysis. Cleavage của photoproduct với cyanogen bromide hoặc S. aureus V8 proteinase dẫn đến sự phân tách chọn lọc của trái phiếu amide trong calmodulin polypeptide mà không có bất kỳ sự phân tách của các phối tử peptide
In this review, we first describe the mechanisms by which the epidermal
growth factor receptor generates a Ca
signal and, subsequently, we com-pile the available experimental evidence regarding the role that the
⁄calmodulin complex, formed after the rise in cytosolic free Ca
concentration, exerts on the receptor.
An immuno-homologue of maize Ca2+/calmodulin (CaM)dependent protein kinase with a molecular mass of 72 kDa was identiﬁed in pea. The pea kinase (PsCCaMK) was upregulated in roots in response to low temperature and increased salinity. Exogenous Ca2+ application increased the kinase level and the response was faster than that obtained following stress application. Low temperaturemediated, but not salinity-mediated stress kinase increase was inhibited by the application of EGTA and W7, a CaM inhibitor....
A common physiological response of organisms to environmental stresses
is the increase in expression of heat shock proteins (Hsps). In insects, this
process has been widely examined for heat stress, but the response to cold
stress has been far less studied.
Thimet oligopeptidase (EC 188.8.131.52; EP24.15) was originally described as
a neuropeptide-metabolizing enzyme, highly expressed in the brain, kidneys
and neuroendocrine tissue. EP24.15 lacks a typical signal peptide sequence
for entry into the secretory pathway and is secreted by cells via an uncon-ventional and unknown mechanism.