Casein phosphopeptides (CPPs) form aggregated complexes with calcium
phosphate and induce Ca
influx into HT-29 cells that have been shown
to be differentiated in culture. The relationship between the aggregation of
CPPs assessed by laser light scattering and their biological effect was stud-
Milk evolved as a means of providing for the postnatal needs of the developing
mammalian neonate. Any perception that milk is a simple, homogeneous fluid does
not adequately acknowledge the remarkable array of complex activities and
functionalities of its constituent components.
Calcium phosphate nanoclusters are equilibrium particles of defined chemi-cal composition in which a core of amorphous calcium phosphate is
sequestered within a shell of casein phosphopeptides. Sequence analyses
and a structure prediction method were applied to secreted phosphopro-teins of known importance in controlling calcification, and eight noncasein
phosphoproteins were identified as containing one or more subsequences
capable of forming nanoclusters.