This volume is the first book-length survey of caveolae and lipid rafts. Interest has
developed rapidly in the role of these surface microdomains in such diverse fields
as transmembrane signaling, cell locomotion, vascular relaxation, senescence, and
the uptake and exit from cells of viruses and bacteria. Individual chapters in this
volume cover areas as diverse as the forces that induce and maintain membrane
invaginations, and the clinical relevance of multiprotein complexes at the cell surface,
defects in which are associated with cancer, and Alzheimer’s and prion-dependent
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài:
The caveolae-mediated sv40 entry pathway bypasses the golgi complex en route to the endoplasmic reticulu
Caveolae are nearly ubiquitous plasma membrane domains that in adipo-cytes vary in size between 25 and 150 nm. They constitute sites of entry
into the cell as well as platforms for cell signalling. We have previously
reported that plasma membrane-associated caveolae that lack cell surface
access can be identified by electron microscopy.
It is now generally accepted that factor XII (FXII) binds to
cellular surfaces in the vascular system. One of the suggested
receptors of this binding is the glycosylphosphatidylinositol-anchored urokinase-like plasminogen activator (u-PAR)
harbored in caveolae/lipid rafts. However, binding of FXII
to human umbilical vein endothelial cells (HUVEC) has
never been shown to be localized to these specialized mem-brane structures. Using microscopical techniques, we here
report that FXII binds to specific patches of the HUVEC
plasma membrane with a high density of caveolae....
We havemade a comprehensive and quantitative analysis of
the lipid composition of caveolae from primary rat fat cells
and compared the composition of plasma membrane inside
and outside caveolae. We isolated caveolae from purified
to disrupt the membrane, or extraction with nonionic
detergent, followed by density gradient ultracentrifugation.
The carbonate-isolated caveolae fraction was further
immunopurified using caveolin antibodies.
Caveolae are plasma membrane invaginations with several
functions, one of which appears to be to organize receptor
mediated signalling. Here we report that in primary human
subcutaneous adipocytes the insulin receptor was localized
to caveolae by electron microscopy/immunogold detection
and by isolating caveolae from plasma membranes.
The caveolin gene family has three members in vertebrates: caveolin-1, caveolin-2, and caveolin-3. So far, most caveolin-related research has been conducted in mammals, but the proteins have also been found in other animals, including Xenopus laevis, Fugu rubripes, and Caenorhabditis elegans. Caveolins can serve as protein markers of caveolae (‘little caves’), invaginations in the plasma membrane 50-100 nanometers in
In 1996, the Committee on Population of the National Research Council
sponsored a novel workshop on the biodemography of aging, which
resulted in the volume Between Zeus and the Salmon: The Biodemography
of Longevity (National Research Council, 1997). The workshop and its
report, which considered the continuing increases in human life span in a
broad biological context, launched a new phase in studies of human aging
at the population level.
The peptide hormone endothelin transmits various signals
throughGprotein-coupled receptors, the endothelin type A
(ETAR) and B (ETBR) receptors. Caveolae are specialized
lipid rafts containing polymerized caveolins. We examined
the interaction of ETBR with caveolin-1, expressed in Sf9,
COS-1, and HEK293 cells, and its effects on the subcellular
distribution and the signal transduction of ETBR.