Cell harvesting

Xem 1-20 trên 21 kết quả Cell harvesting
  • We examined the effects ofEscherichia coliribosomal pro-tein S12 mutations on the efficiency of cell-free protein syn-thesis. By screening 150 spontaneous streptomycin-resistant isolates from E. coliBL21, we successfully obtained seven mutants of the S12 protein, including two streptomycin-dependent mutants. The mutations occurred at Lys42, Lys87, Pro90 and Gly91 of the 30S ribosomal protein S12. We prepared S30 extracts frommutant cells harvested in the mid-log phase.

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  • Loss of tumor suppressors contributes to numerous cancer types. Many, but not all, proteins encoded by tumor suppressor genes have antiproliferative activity and halt cell-cycle progression. In this chapter, we present three methods that have been utilized to monitor the antimitogenic action exerted by tumor suppressors. Tumor suppressor function can be demonstrated by colony formation assays and acquisition of the flat-cell phenotype.

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  • The purpose of this book is to provide an up-to-date view of latest research advances in the design of efficient small-scale energy harvesters through contributions of internationally recognized researchers. The book covers the physics of the energy conversion, the elaboration of electroactive materials and their application to the conception of a complete microgenerator, and is organized according to the input energy source.

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  • Diatoms differ from higher plants by their antenna system, in terms of both polypeptide and pigment contents. A rapid isolation procedure was designed for the membrane-intrinsic light harvesting complexes (LHC) of the diatom Phaeodactylum tricornutumto establish whether different LHC subcomplexes exist, as well to determine an uneven distribution between them of pigments and polypeptides.

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  • Biofuels Engineering Process Technology has many contents: Harvesting Energy from Biochemical Reactions, Microbial Modeling of Biofuel Production, Biofuel Feedstocks, Ethanol Production, Biodiesel, Biological Production of Hydrogen, Microbial Fuel Cells.

    pdf385p doremon3244 04-06-2014 28 18   Download

  • For cell-based bone tissue engineering, various tissues derived cells are utilized since osteogenic cells can be harvested from bone marrow, periosteum, and adipose tissue, though recent studies indicate that bone marrow stromal cells (BMSCs, bone marrow derived multipotent mesenchymal stromal cells, or mesenchymal stem cells) are the most reliable cell source because of their superior osteogenic ability (Hayashi et al., 2008). However, it is difficult to obtain adequate numbers of transplantable BMSCs from bone marrow aspirates, as they are rare in the bone marrow (less than 0.

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  • Storage of putative stem/progenitor cells is also an attractive research target in tissue engineering. Collection of cells for tissue engineering is less invasive than conventional tissue or organ transplantation. However, repetitive collection of cells can stress patients. If the stem/progenitor cells could be stored, it is possible that they could be repeatedly used for future therapy, reducing the burden and the cost to patients. The concept of banking stem/progenitor cells in storage is not new. Stem/progenitor cell populations decrease in size with age (D'Ippolito et al.

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  • Phototrophy is the process by which organisms trap photons and store energy as chemical energy in the form of adenosine triphosphate (ATP). ATP transports chemical energy within cells for metabolism. There are three major types of phototrophy: Oxygenic and Anoxygenic photosynthesis, and Rhodopsin-based phototrophy. Photosynthesis is a chemical process that converts carbon dioxide into different organic compounds using solar energy. Oxygenic and anoxygenic photosynthesis undergo different reactions in the presence and absence of light (called light and dark reactions, respectively).

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  • Over the last decade, PV technology has shown the potential to become a major source of power generation for the world – with robust and continuous growth even during times of financial and economic crisis. That growth is expected to continue in the years ahead as worldwide awareness of the advantages of PV increases. At the end of 2009, the world’s PV cumulative installed capacity was approaching 23 GW. One year later it was 40 GW. In 2011, more than 69 GW are installed globally and...

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  • The proliferation of low-power and ultralow-power electronics has enabled a rapid growth of autonomous devices that ranges from consumer electronics and nomad devices to autonomous sensors and sensor networks used in industrial and military environments. Hence, a wide range of application domains has been impacted by such technologies (aeronautic, civil engineering,biomedical engineering, home automation, etc.).

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  • Only human females and some other higher primates have a regular monthly menstrual cycle. Humans, however, are able to mate at any time. Females of almost all other mammal species will mate only at specific times during a year. These times are known as estrus or “heat.” It is only during estrus that egg cells are released for fertilization. Estrus occurs in wolves and deer once a year, in dogs usually twice a year, in cows and horses once a month, and in rats and mice every few days. Ovulation, mating, and fertilization occur on a specific timetable.

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  • Perhaps unsurprisingly, the ethical debate on cloning tended to blur the lines of separation between the church and state, to an extent that had not been so obvious at the international level for some time. This highly charged issue challenges the bases of many religious beliefs and places science and religion at loggerheads. Questions were raised such as, whether it is appropriate to allow for the creation and destruction of embryos for the sole purpose of harvesting stem cells which may save the life of a sick person is at the very heart of this debate.

    pdf321p thankinhphanliet 21-12-2012 23 3   Download

  • There are currently 11 state buy-down programs for renewable energy technologies, all of which have been initiated within the past several years. Nearly all of these programs are funded by public benefits funds and administered by the state’s energy office, third-party fund administrator, or individual utilities. All of the buy-down programs fund PV installations, with several states targeting PV exclusively. About half of the programs also support wind technology development. A few programs include solar thermal systems or fuel cells as eligible technologies.

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  • We investigated the metabolism ofl-lactate in mitochondria isolated from potato tubers grown and saved after harvest in the absence of any chemical agents. Immunologic analysis by western blot using goat polyclonal anti-lactate dehydrogenase showed the existence of a mitochondrial lactate dehydrogenase, the activity of which could be measured photometrically only in mitochondria solubilized with Triton X-100.

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  • The photosynthetic protein complexes in plants are located in the chloroplast thylakoid membranes. These membranes have an ultrastructure that consists of tightly stackedÔgranaÕ regions interconnectedbyunstackedmembrane regions.The structure of isolated granamembranes has been studiedhere by cryo-electron microscopy. The data reveals an unusual arrangement of the photosynthetic protein complexes, staggered over two tightly stacked planes.

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  • Cambridge researchers César Milstein and George Köhler won a Nobel Prize in 1984 for their work developing monoclonal antibodies (mAbs). Each mAb is a specific antibody, a ‘magic bullet’ protein that targets and attacks one particular substance or cell. Milstein and Köhler found a way to produce these magic bullets in unlimited quantities. mAbs are proteins produced in the laboratory from the cells of the immune system that make antibodies.

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  • The storage of tissue-engineered products is an attractive target for technological development. Since tissue-engineered products usually consist of living cells, technical issues include the limited shelf life of the cells and the use of specialized conditions for transportation. Without storage, treatment with cells requires timely harvesting from the donor, which significantly affects the availability of tissue-engineered products.

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  • Concerns about the level of technological capability make it much easier to build a consensus regarding the need to protect human dignity by preventing experimentation in cloning.

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  • An extracellular lipase, LipA, extracted fromAcinetobacter sp. RAG-1 grown on hexadecane was purified and proper-ties of the enzyme investigated. The enzyme is released into the growth medium during the transition to stationary phase. The lipase was harvested from cells grown to sta-tionary phase, and purified with 22% yield and 10-fold purification. The protein demonstrates little affinity for anionexchange resins,withcontaminatingproteins removed by passing crude supernatants over a Mono Q column....

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  • Chapter 9 - Cellular respiration: harvesting chemical energy. After studying this chapter you will be able to: Explain in general terms how redox reactions are involved in energy exchanges; name the three stages of cellular respiration, for each, state the region of the eukaryotic cell where it occurs and the products that result; in general terms, explain the role of the electron transport chain in cellular respiration;...

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