Preparation and characterisation of attenuated cold-adapted inﬂuenza A reassortants derived from the A/Leningrad/134/17/57 donor strain presents the assay was capable of detecting interference where the infectivity ratio for A/Len/17 and A/Beij/32 was 1:7. Progeny viruses were characterised genetically by ampliﬁcation of deﬁned regions within each of the six internal genes by PCR and identiﬁcation of the products by restriction enzyme analysis.
The essential oil industry is under threat from numerous issues at present. Rising
agricultural input costs, particularly petrochemical based fertilizers and fuels are squeezing
farmers’ profit margins. Rising food prices and the increasing value of housing and industrial
land prices is creating competition for land, particularly around populated areas. Adverse
weather conditions and changing climate are creating both short and long term problems.
Poor crop yields are occurring much more regularly than before because of unusually heavy
rain, hail, floods, heat-waves and cold snaps....
Soil contamination has severely increased over the last decades, mainly due to petroleum hydrocarbons, solvents, pesticides, lead and other heavy metals from industrial wastes and human activities. The critical point regarding contaminated soil monitoring is the intrinsic difficulty in defining fixed monitoring variables and indicators as the establishment of any a priori criterion and threshold for soil quality can be still considered subjective.
The different sources of potential confounding in time series studies of air pollution and
mortality can be broadly classiﬁed as either measured or unmeasured. Important measured
confounders include weather variables such as temperature and dewpoint temperature. Daily
temperature measurements are readily available for metropolitan areas in the USA and numer-
ous studies have demonstrated a relationship between temperature and mortality which is gen-
erally positive for warm summer days and negative for cold winter days (e.g. Curriero et al.
Genetic selection has been used to isolate second-site suppressors of a
defective cold-sensitive initiation factor I (IF1) R69L mutant ofEscherichia
coli. The suppressor mutants specifically map to a single rRNA operon on a
plasmid in a strain with all chromosomal rRNA operons deleted.
Translation initiation factor 1 (IF1) is an essential protein in prokaryotes.
The nature of IF1 interactions with the mRNA during translation initiation
on the ribosome remains unclear, even though the factor has several known
functions, one of them being RNA chaperone activity. In this study, we
analyzed translational gene expressionin vivo in two cold-sensitive chromo-somal mutant variants of IF1 with amino acid substitutions, R40D and
R69L, using two different reporter gene systems.
Plants inhabiting high alpine and nival zones are considered as living in an extreme
Extreme environments have been attractive for explorers for centuries, and nowadays
they also attract tourists. Fortunately biological science is becoming increasingly
aware that these remote habitats provide challenging questions that will help to
understand the limits of life functions.
Vehicle test system and exhaust multi gas equipment for transient measurements in cold climate A pilot study to demonstrate regional competence of advanced measurement technology for vehicle test companies.
Harrison's Internal Medicine Chapter 17. Fever and Hyperthermia
Fever and Hyperthermia: Introduction Body temperature is controlled by the hypothalamus. Neurons in both the preoptic anterior hypothalamus and the posterior hypothalamus receive two kinds of signals: one from peripheral nerves that transmit information from warmth/cold receptors in the skin and the other from the temperature of the blood bathing the region. These two types of signals are integrated by the thermoregulatory center of the hypothalamus to maintain normal temperature.
Abomasum. Fourth and last chamber of the ruminant stomach. Abyssopelagic zone. Region of the oceanic zone from approximately 4,000 to 6,000 m; almost constant physical environment; continually dark, cold (4°C), and virtually unchanging in chemical composition.
More and more evidence of global climate warming, sea level rise, droughts, floods and climate extremes are recorded. In Vietnam, the manifestation of climate change (CC) is often considered as changing the level and trend of variation of main climatic elements and sea level. However, Vietnam climate influenced by the characteristics of atmospheric circulation in the Earth climate system.
We found that benomyl, a benzimidazole fungicide, strongly suppressed
the reassembly of cold-depolymerized spindle microtubules in HeLa cells.
Benomyl perturbed microtubule-kinetochore attachment and chromosome
alignment at the metaphase plate. Benomyl also significantly decreased
the distance between the sister kinetochore pairs in metaphase cells and
increased the level of the checkpoint protein BubR1 at the kinetochore
region, indicating that benomyl caused loss of tension across the kineto-chores....
Terrorism is not new to Southeast Asia. For much of the Cold War, the activities of a variety of domestic ethnonationalist and religious militant groups posed a significant challenge to the region’s internal stability. Since the 1990s, however, the residual challenge posed by substate militant extremism has risen in reaction to