Science often grows exponentially from certain key insights, and the insight Marvin Minsky
had in 1955 is no exception. While working as a postdoctoral fellow at HarvardUniversity,
Minsky sought a method to reduce the high level of light scatter that made high contrast imag‐
ing of thick specimens impossible by standard light microscopy.
Tham khảo sách 'confocal laser microscopy - principles and applications in medicine, biology, and the food sciences by lagali contributors', y tế - sức khoẻ, y học thường thức phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả
Ever since the invention of laser by Schawlow and Townes in 1958, various innovative ideas of laser-based applications emerge very year. At the same time, scientists and engineers keep on improving laser's power density, size, and cost which patch up the gap between theories and implementations. More importantly, our everyday life is changed and influenced by lasers even though we may not be fully aware of its existence.
In 1665, a book was published that inaugurated the use of the microscope to
investigate the natural world. The author was Robert Hooke, a talented artist,
architect, and amateur scientist. Hooke wrote Micrographia: Or Some
Physiological Descriptions of Minute Bodies Made by Magnifying Glasses with
Observations and Inquiries Thereupon, at the behest of the newly chartered
Royal Society in London, for whom he was working as curator of scientific
experiments. In Micrographia, he presented the first detailed observations of
everyday objects made with his self-constructed light microscope....
In the eight years since publication of the first edition, enormous progress has been
made in the field of skin imaging and analysis. Because of the broad array of new
methods now available to the research scientist—and more and more to the clinically-
oriented dermatologist—we were able to widen the scope of this book from
the skin surface to the entire skin. This is also reflected in the change of the volume
title, Bioengineering of the Skin: Skin Imaging and Analysis.
Hence, this second edition is a major revision of the first edition, with more
than 30 new chapters added....
The uptake by mammalian cells of phosphorothioate oli-gonucleotides was compared with that of their respective
complexes or conjugates with cationic, cell-penetrating
model peptides of varying helix-forming propensity and
amphipathicity. An HPLC-based protocol for the synthesis
and purification of disulfide bridged conjugates in the 10–
100 nmol range was developed. Confocal laser scanning
microscopy (CLSM) in combination with gel-capillary
electrophoresis and laser induced fluorescence detection
(GCE-LIF) revealed cytoplasmic and nuclear accumula-tion in all cases. ...
Jurkat T lymphocytes were treated with daunorubicin and
WP631, a daunorubicin-based DNAbinding agent, in
experiments aimed to analyze cellular uptake of these drugs
and their effect on cell viability. WP631 was taken up more
slowly than daunorubicin, but laser confocal microscopy
and spectrofluorometricquantificationshowed that thedrug
accumulated in the cells. Despite the slow uptake rate, the
antiproliferative capacity of WP631 (measured as IC50after
a 72-h continuous treatment) was greater than that of