Density fractions

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  • An Introduction to the Study of Mineralogy is a collection of papers that can be easily understood by a wide variety of readers, whether they wish to use it in their work, or simply to extend their knowledge. It is unique in that it presents a broad view of the mineralogy field. The book is intended for chemists, physicists, engineers, and the students of geology, geophysics, and soil science, but it will also be invaluable to the more advanced students of mineralogy who are looking for a concise revision guide....

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  • In addition to the major carbohydrate moieties of the glycosylphosphatidylinositol (GPI) anchor, we report that Plasmodiumfalciparummerozoite surface protein1 (MSP-1) bearsO-GlcNAc modifications predominantly in b-ano-meric configuration, in both the C- andN-terminal portions of the protein. Subcellular fractionation of parasitized erythrocytes in the late trophozoite/schizont stage reveals that GPI-anchored C-terminal fragments of MSP-1 are recovered in Triton X-100 resistant, low-density membrane fractions....

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  • Using thein situ liver model system, we have recently shown that, after cholera toxin binding to hepatic cells, cholera toxin accumulates in a low-density endosomal compartment, and then undergoes endosomal proteoly-sis by the aspartic acid protease cathepsin-D [Merlen C, Fayol-Messaoudi D, Fabrega S, El Hage T, Servin A, Authier F (2005)FEBS J272, 4385– 4397]. Here, we have used a subcellular fractionation approach to address the in vivo compartmentalization and cytotoxic action of cholera toxin in rat liver parenchyma....

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  • We havemade a comprehensive and quantitative analysis of the lipid composition of caveolae from primary rat fat cells and compared the composition of plasma membrane inside and outside caveolae. We isolated caveolae from purified plasmamembranesusingultrasonication incarbonatebuffer to disrupt the membrane, or extraction with nonionic detergent, followed by density gradient ultracentrifugation. The carbonate-isolated caveolae fraction was further immunopurified using caveolin antibodies.

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  • The protein-modifying agent arsenite stimulates glucose uptake in 3T3-L1 adipocytes. In the current study we have analysed the signalling pathways that contribute to this response. By subcellular fractionation we observed that arsenite, like insulin, induces translocation of the GLUT1 and GLUT4 glucose transporters from the low-density membrane fraction to the plasma membrane. Arsenite did not activate early steps of the insulin receptor (IR)-signalling pathway and the response was insensitive to inhibition of phosphatidylinositol-3¢-kinase (PI-3¢)kinaseby wortman-nin. ...

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