Xem 1-20 trên 84 kết quả Design of the gene
  • Once you have your restriction enzymes chosen, it is time to design the final complete gene The multiple cloning site (or whatever plasmid you are cloning into) should already have the 5’ portion of the gene intact (i.e. RBS, spacer, Met) Sequences must be in frame

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  • The wide geographical distribution of the rice plant ( Oryza sativa L.) and its long history of cultivation in Asian countries have led to the development of a great diversity of varietal types. Similarly, workers in various rice-growing countries use different terms to designate identical morphological and physiological characters, agronomic traits, gene symbols, and cultural practices. Whereas varietal diversity in germ plasm is desired in rice breeding, variations in nomenclature hinder scientific communication among the workers.

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  • Most of the development of online financial services has been reactive, doing the minimum amount of work to try and frustrate the attacks which are observed. It has also been quite piecemeal and uncoordinated. Almost all of the defences have a simple attacker model which only considers those attacks which their prospective target has experienced in the wild. Some of these systems manage to achieve their (fairly limited) goals, but many of them are only partially effective at best.

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  • An overview of the research plan is shown in Figure 3. Paper I covers the background in the form of literature review. Paper II focuses on the optimisation of hot water treatment prior to alkaline peroxide bleaching and on the mechanical refining of hot water treated straw. Paper III focuses on the optimisation of alkaline peroxide bleaching and Paper IV focuses on the chemical pre-treatment/storage of wheat straw. Paper V evaluates the obtained results.

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  • Let’s see what the dinner party will involve? (See illustration of work breakdown structure) It is a lot more complicated than one would think. Major tasks include setting up and running the dinner, entertainment, and silent auction, finding a dinner speaker and clean up after the dinner is over. Beneath these major tasks are subtasks broken into 16 discrete categories. Each of the subtasks will break down into repeated or “one-off” steps. The complete list of steps with milestones runs to 75 entries. (See illustration of task list). ...

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  • important regulators of biological processes in animals and plants. MiRNAs regulate gene expression at the posttranscriptional level by binding to mRNAs and either inhibit translation or modify the stability of the mRNA. Due to the important biological role of miRNAs it is of great interest to study their expression level in the cells. Furthermore, miRNAs have been associated with cancer and other diseases [1] and miRNA expression can help in the diagnosis and prognostic of human disease [2,3].

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  • The spontaneous acquisition of resistance to a variety of unrelated cytotoxic compounds has important implications in medical treatment of infectious diseases and anticancer therapy. In the yeast Saccharomyces cerevisiaethis pheno-menon is caused by overexpression of membrane efflux pumps and is called pleiotropic drug resistance. We have found that allelic forms of the genes for the transcription activators Pdr1p and Pdr3p, designated PDR1-12and PDR3-33,respectively, mediate resistance to diazaborine....

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  • For example, in the case of a multinational corporation, it might be reason- able to assume a high degree of control and coordination in the administration of the cluster of subnets of the corporation’s intranet. The administrators of this intranet might very well be able to design and manage the whole network of event servers deployed on their subnets, and thus it might be a good idea to adopt a hierarchical architecture within the intranet. Of course, the intranet would connect to other networks outside of the influence of the administrators.

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  • The third edition of Principles of Tissue Engineering attempts to incorporate the latest advances in the biology and design of tissues and organs and simultaneously to connect the basic sciences — including new discoveries in the field of stem cells — with the potential application of tissue engineering to diseases affecting specific organ systems.

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  • Our Management major programme combines critical thinking skills with solid theory. You’ll learn skills in decision-making and problem solving, study the design of organisations and the way work is managed, long- term business strategies, control systems, and culture and politics in organisations. You’ll also build up an understanding of management information, and issues specific to managers of national and international environments. Massey’s Management qualifications are designed to meet your individual career and industry needs.

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  • As explained in Sales that are generally nontaxable, charges for composed type only or reproduction proofs of composed type only, are not taxable. Charges for the composition of type are considered charges for a service, unless the charges are part of the sale of printed matter. “Composed type” includes type together with lined borders and plain, straight, fancy, or curved lines. Composed type also includes charts, tables, graphs, and similar methods of providing information.

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  • Genotypes describe the specific alleles at a particular locus. For example, there are three common alleles (E2, E3, E4) of the apolipoprotein E (APOE) gene. The genotype of an individual can therefore be described as APOE3/4 or APOE4/4 or any other variant. These designations indicate which alleles are present on the two chromosomes in the APOE gene at locus 19q13.2. In other cases, the genotype might be assigned arbitrary numbers (e.g., 1/2) or letters (e.g., B/b) to distinguish different alleles.

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  • The Consumer Federation of America (CFA) and the National Credit Reporting Association (NCRA) designed the details of this study with advice from legal counsel to ensure the methodology would comply with the requirements of the Fair Credit Reporting Act, Gramm Leach Bliley Act, and other consumer privacy laws. From the outset, each organization was mindful of the ethical spirit and intent of these consumer protection and privacy laws.

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  • As indicated above, in some instances, the Commissioner may decide to utilise powers conferred under section 24 of the Data Protection Acts and order an inspection team comprised of authorised officers to arrive unannounced at the premises of a particular data controller or data processor. In such cases, authorised officers will carry official photo identification and a signed letter from the Commissioner on logo-headed paper.

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  • “Designing Outdoor Sculpture Today for Tomorrow” was written for sculptors, owners, art managers, architects, landscape architects, engineers, fabricators, foundry personnel, conservators, community groups and others who care about outdoor sculpture. Allies in the broader field of public art will find this a useful tool too. This guide was written by their colleagues—Glenn Wharton, conservator, with assistance from Rita Roosevelt, public art specialist, and Mark Rabinowitz, sculptor and conservator.

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  • Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene

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  • With its focus on cost and third party payment, the regulatory program has also managed to shift the public debate. The historical focus on caring for an individual patient has been subsumed in discussions of pricing, cost con- trol, and the merits of using a variety of delivery systems for expanding the third party payments system to an ever- increasing fraction of the population, legal or not. The collateral damage has been high. People have lost sight of the important role that involved consumers spending their own money play in controlling system costs and quality.

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  • There are also institutional issues to resolve. A path-dependent technology pattern has developed in the energy field that encourages the current centralized generation model of energy supply. When shifting away the capital investments in technology from centrally planned facilities toward distributed generation, renewable energy technologies and their prospective customers must fight against a tide of perceptions, practices, and market structures designed to facilitate the old central-station ways.

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  • The oldest and most basic form of animation used up to the present is 2D animation. The process of 2D animation is done by combining multiple sets of two-dimensional hand-drawn objects and placing them in a sequence and in a manner that creates an illusion that the drawn objects are in fact moving. With the advancement of computer technology, 2D animation has evolved into more than just handmade drawings. Today, these drawings are given more depth by scanning each frame individually and further manipulation and enhancement are processed by software applications.

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  • The most basic yet comprehensive component of a strong institutional image is a unified visual presentation. Collectively, all elements of the University of Kentucky must present to the world a consistent image that reflects our quality, professionalism and mission. The University of Kentucky name carries heavy responsibility. It stands as the sum of the institution’s many parts and embodies the authentic, recognizable essence of this institution. It must reinforce to many diverse audiences a definitive and unique impression.

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