Xem 1-20 trên 39 kết quả Detection of bacteria
  • Milk has nutrients that make it suitable for the rapid multiplication of bacteria that cause spoilage. Unhygienic production, poor handling and undesirable practices such as addition of water or other substances can introduce bacteria or germs that cause spoilage. The resulting wastage can make you lose profits that you would have otherwise made. Unhygienic handling may introduce disease-causing bacteria into the milk and this can also adversely affect human health.

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  • Computer Tomography (CT) is considered the gold standard for assessing the morphological changes of lung parenchyma. Although novel CT techniques have substantially decreased the radiation dose, radiation exposure is still high. Magnetic Resonance Imaging (MRI) has been established as a radiation- free alternative to CT for several lung diseases, but its role in infectious diseases still needs to be explored further. Therefore, the purpose of our study was to compare MRI with high resolution CT (HRCT) for assessing pulmonary tuberculosis.

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  • Sepsis is a complex syndrome characterized by systemic inflammation in response to infection. It is a significant health problem with an estimated 750,000 new cases in the USA annually [1]. It is also the third leading cause of death in developed countries, equalling the number of fatalities from acute myocardial infarction. The incidence of sepsis has increased in recent decades and is predicted to continue to rise. The high sepsis-related mortalities and the burden on healthcare systems means there is an urgent need to improve the diagnosis and management of sepsis patients....

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  • The fact that the gut microfl ora can be altered for the benefi t of human health has motivated the development of new functional food ingredients. A probiotic is a live microbial food supplement, which benefi cially affects the host by improving its intestinal microbial bal- ance (Fuller 1989). A prebiotic is defi ned as a nondigestible food ingredient that benefi cially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon, and thus improves host health (Gibson and Roberfroid 1995)....

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  • The CD53 antigen is a member of the tetraspanin membrane protein family that is expressed in the lymphoid-myeloid lineage. We have studied the implication of CD53 antigen in signal transduction by determining the effect of its ligation on the c-Jun N-terminal kinase (JNK) in different cell types. Ligation of the rat or human CD53 antigen induces a threeto fourfold transient activation of JNK activity that peaks at 3–5 min. The effect was detected by assaying the endogenous or exogenous (transfected) JNK activity....

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  • The lipolysis stimulated receptor (LSR) recognizes apo-lipoprotein B/E-containing lipoproteins in the presence of free fattyacids, and is thought tobe involved in the clearance of triglyceride-rich lipoproteins (TRL). The distribution of LSR in mice was studied by Northern blots, quantitative PCR and immunofluorescence. In the adult,LSRmRNA was detectable in all tissues tested except muscle and heart, and was abundant in liver, lung, intestine, kidney, ovaries and testes. During embryogenesis, LSR mRNA was detectable at 7.

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  • Intrusion detection systems and vulnerability scanners provide an additional layer of network security.While firewalls permit or deny traffic based on source, destination, port, or other criteria, they do not actually analyze traffic for attacks or search the network for existing vulnerabilities. In addition, firewalls typically do not address the internal threat presented by “insiders.

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  • Mucin, a major component of mucus, is a highly O-glycosylated, high-molecular-mass glycoprotein extensively involved in the physiology of gastrointestinal mucosa. To detect and characterize mucins derived from site-specific mucous cells, we developed a monoclonal antibody, designated PGM34, by immunizing a mouse with purified pig gastric mucin.

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  • We investigated structural and functional aspects of the first mutation in TNNC1, coding for the calcium-binding subunit (cTnC) of cardiac tropo-nin, which was detected in a patient with hypertrophic cardiomyopathy [Hoffmann B, Schmidt-Traub H, Perrot A, Osterziel KJ & Gessner R (2001) Hum Mut17, 524]. This mutation leads to a leucine–glutamine exchange at position 29 in the nonfunctional calcium-binding site of cTnC.

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  • We have developed a simple reporter assay useful for detection and analysis of mutations and agents influencing mRNA biogenesis in a gene length-dependent manner. We have shown that two transcription units sharing the same promoter, terminator and open reading frame, but differing in the length of their 3¢-untranslated regions, are differentially influenced by muta-

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  • The catalytic module ofHypocrea jecorina(previously Trichoderma reesei) Cel7B was homologously expressed by transformation of strain QM9414. Post-translational modi-fications in purified Cel7B preparations were analysed by enzymatic digestions, high performance chromatography, mass spectrometry and site-directed mutagenesis. Of the five potential sites found in the wild-type enzyme, only Asn56 and Asn182 were found to be N-glycosylated.

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  • Caveolae are plasma membrane invaginations with several functions, one of which appears to be to organize receptor mediated signalling. Here we report that in primary human subcutaneous adipocytes the insulin receptor was localized to caveolae by electron microscopy/immunogold detection and by isolating caveolae from plasma membranes.

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  • An acidic branched O-polysaccharide was isolated by mild acid degrada-tion of the lipopolysaccharide (LPS) of Proteusgenomospecies 4 and stud-ied by sugar and methylation analyses along with 1 H and 13 C NMR spectroscopy, including 2D COSY, TOCSY, ROESY and H-detected 1 H, 13 C HSQC experiments.

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  • Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleo-tide. These analogs have strong binding affinities for most nucleotide-requiring systems. Their bindings are easily detected by absorption and fluorescence changes in the visi-ble region.Recent years have seendramatic developments in the application of the TNP nucleotide analogs as spectro-scopic probes for the study on the nucleotide-interacting properties of various enzymes and proteins including their mutants.

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  • A technique has been developed to search a proteome database for new members of a functional class of membrane protein. It takes advantage of the highly conserved secondary structure of functionally related membrane proteins. Such proteins typically have the same number of transmembrane domains located at similar relative positions in their polypeptide sequence. This gives rise to a characteristic pattern of peaks in their hydropathy profiles.

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  • The physiological role of sulfoquinovosyl diacylglycerol (SQDG) in photosynthesis was investigated with a SQDG defective mutant (hf-2) of Chlamydomonas reinhardtii that did not have any detectable amount of SQDG. The mutant showed a lower rate of photosystem II (PSII) activity by  40% and also a lower growth rate than those of the wildtype. Results of genetical analysis of hf-2 strongly suggest that the SQDG defect and the lowered PSII activity are due to a single gene mutation.

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  • With the aim to distinguish between local and global conformational changes induced by trifluoroethanol in RNase A, spectroscopic and activity measurements in combination with proteolysis by unspecific proteases have been exploited for probing structural transitions of RNase A as a function of trifluoroethanol concentration. At 30% (v/v) trifluoroethanol (pH 8.0; 25 °C), circular dichroism and fluorescence spectroscopy indicate a cooperative collapse of the tertiary structure of RNase A coinciding with the loss of its enzymatic activity....

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  • In order to evaluate the ability of the cell-penetrating a-helical amphipathic model peptide KLALKLALKALK AALKLA-NH2 (MAP) to deliver peptide nucleic acids (PNAs) into mammalian cells, MAP was covalently linked to the 12-mer PNA 5¢-GGAGCAGGAAAG-3¢ directed against themRNAof the nociceptin/orphaninFQ receptor. The cellular uptake of both the naked PNA and its MAP-conjugate was studied by means of capillary electrophoresis combined with laser-induced fluorescence detection, confo-cal laser scanningmicroscopyandfluorescence-activatedcell sorting. ...

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  • The ultimate step in Alzheimer’s disease Abgeneration involves c-secretase, which releases Abfrom its membrane-bound precursor. Asimilar presenilin-dependent proteolytic activity is implicated in the release of theNotch intracellular domain. We have developed a novel assay forc-secretase activity based on green fluorescent protein detection. This involves cotransfection of a substrate-activator based on the amyloid precursor protein or the Notch sequence and a fluorescent reporter gene.

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  • Duckd2-crystallin is a soluble tetrameric lens protein. In the presence of guanidinium hydrochloride (GdnHCl),it undergoes stepwise dissociation and unfolding. Gel-filtra-tion chromatography and sedimentation velocity analysis has demonstrated the dissociation of the tetramer protein to a monomeric intermediate with a dissociation constant of 0.34lM 3 . Dimers were also detected during the dissociation and refoldingprocesses.

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