Alter the appearance, aroma, texture and flavor of a product.
Deliver nutrients more effectively to the body or to make a food that is
difficult to digest a bit easier to swallow.
Accelerate the good fermentation time, giving a finished product in weeks
instead of months.
Whilst herbivory is widespread in the animal kingdom, no vertebrates and few invertebrates are capable of synthesizing cellulose- or hemicellulose-digesting enzymes. Instead, herbivores have evolved symbiotic associations with microorganisms....
In ruminants, some leaf-eating animals, and some insects, defensive lyso-zymes have been adapted to become digestive enzymes, in order to digest
bacteria in the stomach. Digestive lysozyme has been reported to be resis-tant to protease and to have optimal activity at acidic pH. The structural
basis of the adaptation providing persistence of lytic activity under severe
gastric conditions remains unclear.
We purified eel hatching enzyme (EHE) from the hatching liquid of Japa-nese eelAnguilla japonicabelonging to Elopomorpha to a single band on
SDS⁄PAGE. TOF-MS analysis revealed that the purified EHE contained
several isozymes with similar molecular masses.
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The structural carbohydrates that constitute plant fibre represent a major feed resource. Herbivorous animals, unable to produce fibre-degrading enzyme systems of their own, have evolved a range of strategies (Hume and Sakaguchi, 1991) to make use of a consortium of microbes, including bacteria, protozoa and anaerobic fungi, for this purpose...
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination
We purified two hatching enzymes, namely high choriolytic enzyme (HCE;
EC 188.8.131.52) and low choriolytic enzyme (LCE; EC 184.108.40.206), from the
hatching liquid ofFundulus heteroclitus, which were named FundulusHCE
(FHCE) and FundulusLCE (FLCE). FHCE swelled the inner layer of egg
envelope, and FLCE completely digested the FHCE-swollen envelope.
Crustacean serine proteases (Brachyurins, EC 220.127.116.11) exhibit a wide
variety of primary specificities and no member of this family has been
reported for spiny lobsters. The aim of this work was to study the diversity
of trypsins in the digestive gland ofPanulirus argus.
The hatching enzyme of oviparous euteleostean fishes consists of two
metalloproteases: high choriolytic enzyme (HCE) and low choriolytic
enzyme (LCE). They cooperatively digest the egg envelope (chorion) at the
time of embryo hatching. In the present study, we investigated the hatching
of embryos of the ovoviviparous black rockfish Sebastes schlegelii.
Carboxypeptidases were purified fromguts of larvae of corn
earworm (Helicoverpa armigera), a lepidopteran crop pest,
by affinity chromatography on immobilized potato carb-oxypeptidase inhibitor, and characterized by N-terminal
sequencing. A larval gut cDNA library was screened using
probes based on these protein sequences. cDNA HaCA42
encoded a carboxypeptidase with sequence similarity to
enzymesofclan MC [Barrett,A.J.,Rawlings,N. D.&
Woessner, J. F. (1998)Handbook of Proteolytic Enzymes.
Academic Press, London.], but with a novel predicted spe-cificity towards C-terminal acidic residues....
The digestive tract of lepidopteran insects is extremely alkaline. In the pres-ent work, molecular adaptation of amylolytic enzymes to this environment
was investigated in the flour moth Ephestia kuehniella, an important
stored-product pest. Three digestivea-amylases [Ephestia kuehniellaa-amy-lase isoenzymes 1–3 (EkAmy1–3)] with an alkaline pH optimum were puri-fied from larvae and biochemically characterized.
There are two hatching enzyme homologues in the zebrafish genome:
zebrafish hatching enzymeZHE1andZHE2. Northern blot and RT-PCR
analysis revealed that ZHE1 was mainly expressed in pre-hatching
embryos, whereasZHE2was rarely expressed. This was consistent with the
results obtained in an experiment conducted at the protein level, which
demonstrated that one kind of hatching enzyme, ZHE1, was able to be
purified from the hatching liquid.
(BQ) Part 1 book "BRS Biochemistry, molecular biology and genetics" presents the following contents: Organic chemistry review; protein structure and function; enzymes, biochemistry of digestion; glycolysis; the tricarboxylic acid cycle, electron transport chain, and oxidative metabolism; glycogen metabolism; gluconeogenesis and the maintenance of blood glucose levels,... and other contents.
Problems of constipation, diarrhoea and irritable bowel syndrome are common. Infective diarrhoeal diseases are a significant cause of morbidity and mortality worldwide, especially in infants and children.The management of these conditions is reviewed. • Constipation: mode of action and use of drugs • Diarrhoea (drug treatment importance of fluid and electrolyte replacement) • Inflammatory bowel disease • Irritable bowel syndrome
STOOL BULKING AGENTS Dietary fibre comprises the cell walls and supporting structures of vegetables and fruits.
Akimov and Bezuglov described existing models for the evaluation of
protein digestibility with a major focus on the evaluation of enzymes involved in
protein digestion in stomach and intestine. Moreover, a special emphasis is placed on
the characterization of the intestinal wall peptidases (surface as well as intracellular)
with description of their specificities and their role in overall protein digestion.
PCR-RFLP analysis of beta-lactoglobulin gene locus was carried out on 110 DNA samples of Murrah buffaloes in the present study. A 262 bp fragment enclosing from exon IV to intron IV in b-lg gene was amplified with specific primers. All the 110 DNA samples resulted
in 262 bp product on amplification. The PCR products were subjected for digestion with
Pst1,EcoRI, HindIII and Hae III enzyme. PCR products were not digested by PstI, EcoRI and
HindIII. PCR products when digested with HaeIII enzyme resulted in monomorphic banding
pattern in all the samples.
Snake venoms are cocktails of enzymes and non-enzymatic proteins used
for both the immobilization and digestion of prey. The most common
snake venom enzymes include acetylcholinesterases, L-amino acid oxidases,
serine proteinases, metalloproteinases and phospholipases A2
The design of b-glycosidases with planed substrate speciﬁcity for biotechnological application has received little attention. This is mostly a consequence of the lack of data on the molecular basis of the b-glycosidase speciﬁcity, namely data on the energy of the noncovalent interactions in the enzymetransition state complex.