During the four years since publication of the Fifth Edition of Gene Cloning and DNA
Analysis: An Introduction there have been important advances in DNA sequencing
technology, in particular the widespread adoption of high throughput approaches based
on pyrosequencing. Inclusion of these new techniques in the Sixth Edition has prompted
me to completely rewrite the material on DNA sequencing and to place all the relevant
information—both on the methodology itself and its application to genome sequencing
—into a single chapter.
Forensic DNA Typing charts the progress and development of DNA applied to
criminal forensics, providing vivid demonstrations of the amazing potential of
the method, not only to convict the guilty but also to exonerate the innocent.
John Butler has created a text that caters to all audiences, covering the basics
of DNA structure and function and describing in detail how the techniques are
used. In addition, the extensive use of D.N.A. (Data, Notes, and Application)
Boxes in the text enables the reader to dip in and out as he or she pleases....
Developmental biology: The anatomical tradition The Questions of Developmental Biology Anatomical Approaches to Developmental Biology Comparative Embryology Evolutionary Embryology Medical Embryology and Teratology Mathematical Modeling of Development Principles of Development:
Over the past 20 years, technological advances in molecular biology have
proven invaluable to the understanding of the pathogenesis of human cancer.
The application of molecular technology to the study of cancer has not only
led to advances in tumor diagnosis, but has also provided markers for the
assessment of prognosis and disease progression. The aim of Molecular Analysis
of Cancer is to provide a comprehensive collection of the most up-to-date
techniques for the detection of molecular changes in human cancer.
(BQ) Part 1 book "Principles and techniques of biochemistry and molecular biology" presents the following contents: Basic principles, cell culture techniques, centrifugation, microscopy, molecular biology, bioinformatics and basic techniques, recombinant DNA and genetic analysis, immunochemical techniques.
Populations in Southeast AsiaandSouthChinahave highfrequencies ofa-thalassemia
caused by a-globin gene mutations and/or deletions. This study was designed to find
an efficient and simple diagnostic test for the mutations and deletions. A duplex polymerase
chain reaction (PCR)/denaturing high-pressure liquid chromatography
(DHPLC) was used to detect the mutations and deletions. A blinded study of 110
samples, which included 92 a-thalassemia samples with various genotypes and 18
normal DNA samples, was carried out by the methods.
Learning objectives of this chapter include: Define and distinguish forensic science and criminalistics, Understand the importance of an investigator's understanding of crime laboratory capabilities, Describe the three measures of effectiveness of crime laboratories, Distinguish the Frye test from the Daubert test regarding the admissibility of scientific evidence, Explain the role and importance of DNA analysis in criminal investigation,…
A catalytic turnover of supercoiled DNA (scDNA) transformation medi-ated by topoisomerases leads to changes in the linking number (Lk) of the
polymeric substrate by 1 or 2 per cycle. As a substrate of the topoisomeri-zation reaction it is chemically identical to its product; even a single
catalytic event results in the quantum leap in the scDNA topology.
Nowadays it is difficult to imagine an area of knowledge that can continue developing
without the use of computers and informatics. It is not different with biology, that has
seen an unpredictable growth in recent decades, with the rise of a new discipline,
bioinformatics, bringing together molecular biology, biotechnology and information
The molecular analysis of human cancer is complicated by the difficulty in
obtaining pure populations of tumor cells to study. One traditional method of
obtaining a pure representation has been establishing cancer cell lines from
primary tumors. However, this technique is time consuming and of low yield.
Artifacts of cell culture include the selection of genetic alterations not present
in primary tumors (1,2) and the alteration of gene expression as compared to
primary tumors (3).
Computational synthetic biology has borrowed methods, concepts, and techniques from systems biology
and electrical engineering. Features of tools for the analysis of biochemical networks and the design of electric
circuits have been combined to develop new software, where Standard Biological Parts (physically stored
at the MIT Registry) have a mathematical description, based on mass action or Hill kinetics, and can be
assembled into genetic networks in a visual, “drag & drop” fashion.