During the four years since publication of the Fifth Edition of Gene Cloning and DNA
Analysis: An Introduction there have been important advances in DNA sequencing
technology, in particular the widespread adoption of high throughput approaches based
on pyrosequencing. Inclusion of these new techniques in the Sixth Edition has prompted
me to completely rewrite the material on DNA sequencing and to place all the relevant
information—both on the methodology itself and its application to genome sequencing
—into a single chapter.
Forensic DNA Typing charts the progress and development of DNA applied to
criminal forensics, providing vivid demonstrations of the amazing potential of
the method, not only to convict the guilty but also to exonerate the innocent.
John Butler has created a text that caters to all audiences, covering the basics
of DNA structure and function and describing in detail how the techniques are
used. In addition, the extensive use of D.N.A. (Data, Notes, and Application)
Boxes in the text enables the reader to dip in and out as he or she pleases....
This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications.
The term DNA sequencing refers to methods for determining the order of the nucleotides
bases adenine,guanine,cytosine and thymine in a molecule of DNA. The first DNA sequence
were obtained by academic researchers,using laboratories methods based on 2- dimensional
chromatography in the early 1970s. By the development of dye based sequencing method
with automated analysis,DNA sequencing has become easier and faster.
Over the past 20 years, technological advances in molecular biology have
proven invaluable to the understanding of the pathogenesis of human cancer.
The application of molecular technology to the study of cancer has not only
led to advances in tumor diagnosis, but has also provided markers for the
assessment of prognosis and disease progression. The aim of Molecular Analysis
of Cancer is to provide a comprehensive collection of the most up-to-date
techniques for the detection of molecular changes in human cancer.
DNA chips are gaining increasing importance in different fields ranging from
medicine to analytical chemistry with applications in the latter in food safety
and food quality issues as well as in environmental protection. In the medical
field, DNA chips are frequently used in arrays for gene expression studies (e.g.
This article outlines the basic parameters and current status of the Integrated Data for Event Analysis
(IDEA) project. IDEA provides a comprehensive events framework for the analysis of international
interactions by supplementing the event forms from all earlier projects with new event forms needed
to monitor contemporary trends in civil and interstate politics.
Neuronal PAS domain protein 2 (NPAS2) is a circadian rhythm-associated
transcription factor with two heme-binding sites on two PAS domains. In
the present study, we compared the optical absorption spectra, resonance
Raman spectra, heme-binding kinetics and DNA-binding characteristics of
the isolated fragment containing the N-terminal basic helix–loop–helix
(bHLH) of the first PAS (PAS-A) domain of NPAS2 with those of the
PAS-A domain alone.
Peroxiredoxins belong to a family of antioxidant proteins that neutralize
reactive oxygen species. One member of this family, peroxiredoxin I
(PRDX1), suppresses DNA oxidation. Peroxiredoxin V (PRDX5) has been
cloned as a transcriptional corepressor, as a peroxisomal⁄mitochondrial
antioxidant protein, and as an inhibitor of p53-dependent apoptosis.
T4 DNA ligase is one of the workhorses of molecular biology and used in
various biotechnological applications. Here we report that this ligase,
unlikeEscherichia coliDNA ligase,TaqDNA ligase and Ampligase, is able
to join the ends of single-stranded DNA in the absence of any duplex
DNA structure at the ligation site.
Phosphorylation at multiple sites within the N-terminus of
p53 promotes its dissociation from hdm2/mdm2 and sti-mulates its transcriptional regulatory potential. The large
phosphoinositide 3-kinase-like kinases ataxia telangiectasia
mutated gene product and the ataxia telangectasia and
RAD-3-related kinase promote phosphorylation of human
p53at Ser15andSer20, andare required for theactivationof
p53 following DNA damage.
The tumor suppressor protein, p53, selectively binds to
supercoiled (sc) DNA lacking the specific p53 consensus
binding sequence (p53CON). Using p53 deletion mutants,
we have previously shown that the p53 C-terminal DNA-binding site (CTDBS) is critical for this binding.
The interaction of the homeodomain of the sunflower KNOX protein
HAKN1 with DNA was studied by site-directed mutagenesis, hydroxyl
radical footprinting and missing nucleoside experiments. Binding of
HAKN1 to different oligonucleotides indicated that HAKN1 prefers the
sequence TGACA (TGTCA), with changes within the GAC core more pro-foundly affecting the interaction. Footprinting and missing nucleoside
experiments using hydroxyl radical cleavage of DNA showed that HAKN1
interacts with a 6-bp region of the strand carrying the GAC core, covering
the core and nucleotides towards the 3¢ end....
Ascorbic acid is often regarded as an antioxidantin vivo,
where it protects against cancer by scavenging DNA-dam-aging reactive oxygen species. However, the detailed mech-anism of the action of ascorbic acid on genetic DNA is still
unclear. We examined the effect of ascorbic acid on the
higher-order structure of DNA through real-time observa-tion by fluorescence microscopy. We found that ascorbic
acid generates a pearling structure in single giant DNA
molecules, with elongated and compact regions coexisting
along a molecular chain. ...
HIV-1 reverse transcriptase (RT) has two associated activities, DNA poly-merase and RNase H, both essential for viral replication and validated drug
targets. Although all RT inhibitors approved for therapy target DNA poly-merase activity, the search for new RT inhibitors that target the RNase H
function and are possibly active on RTs resistant to the known non-nucleoside inhibitors (NNRTI) is a viable approach for anti-HIV drug
We report here a new method for inhibition of RNA viruses induced by
dsDNA. We demonstrated that both long dsDNA molecules and short
interfering DNA with a sequence complementary to that of viral RNA
inhibited tobacco mosaic virus expression and prevented virus spread. Also,
the expression of the HIV-1 gp41 gene in HeLa cells was inhibited by com-plementary short interfering DNA.
DNA polymerasec, a mitochondrial replication enzyme of yeasts and ani-mals, is not present in photosynthetic eukaryotes. Recently, DNA polyme-rases with distant homology to bacterial DNA polymerase I were reported
in rice, Arabidopsis, and tobacco, and they were localized to both plastids
Human papillomavirus type 16 (HPV16) DNA replication, which requires
two viral proteins E1 and E2, occurs only in the differentiating epithelium.
Besides the general factors necessary for cellular DNA synthesis, other
unidentified cellular factors are assumed to be involved in the regulation of
HPV DNA replication.
The DNA adenine methylation status on specific 5¢-GANTC-3¢ sites and
its change during the establishment of plant–microbe interactions was
demonstrated in several species ofa-proteobacteria. Restriction landmark
genome scanning (RLGS), which is a high-resolution two dimensional
DNA electrophoresis method, was used to monitor the genomewide change
Host species specificity of the polyomaviruses simian virus
40 (SV40) and mouse polyomavirus (PyV) has been shown
to be determined by the host DNA polymerase a-primase
complex involved in the initiation of both viral and host
DNA replication. Here we demonstrate that DNA repli-cation of the related human pathogenic polyomavirus JC
virus (JCV) can be supportedin vitro by DNA polymerase
a-primase of either human or murine origin indicating that
the mechanism of its strict species specificity differs from
that of SV40 and PyV.