Forensic DNA Typing charts the progress and development of DNA applied to
criminal forensics, providing vivid demonstrations of the amazing potential of
the method, not only to convict the guilty but also to exonerate the innocent.
John Butler has created a text that caters to all audiences, covering the basics
of DNA structure and function and describing in detail how the techniques are
used. In addition, the extensive use of D.N.A. (Data, Notes, and Application)
Boxes in the text enables the reader to dip in and out as he or she pleases....
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report...
Tham khảo luận văn - đề án 'báo cáo y học: "diagnosis of systemic toxoplasmosis with hiv infection using dna extracted from paraffinembedded tissue for polymerase chain reaction: a case report"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems...
Môi trƣờng LB agar, kháng sinh ampicillyn, X-gal, IPTG (bacto tryptone, bacto yeast extract, natri chlorua, agar. Ampicillyn, X-gal, IPTG ) 3.2.5 Các loại hóa chất dùng trong thí nghiệm Hóa chất dùng cho PCR (đƣợc cung cấp bởi công ty Bio-rad) iTaq polymerase MgCl2 dNTPs PCR buffer Các loại hoá chất khác Tryptone Bacto yeast extract Natri chlorua Canxi chlorua X-gal IPTG Kháng sinh Ampicillyn Glucose Tris- HCl pH 8.0 3.2.6 Các loại enzym dùng trong thí nghiệm BamHI với trình tự nhận biế ...
The advances of microelectromechanical systems (MEMS) and devices have been instrumental in the demonstration of new devices and applications, and even in the creation of new fields of research and development: bioMEMS, actuators, microfluidic devices, RF and optical MEMS. Experience indicates a need for MEMS book covering these materials as well as the most important process steps in bulk micro-machining and modeling. We are very pleased to present this book that contains 18 chapters, written by the experts in the field of MEMS. ...
This book contains four parts with 16 chapters. Firstly, an optimal stimulation scheme
for ovaries, particularly natural and minimal stimulation of ovaries, has been
discussed in the first part. Then, one paper analyzed that how many oocytes per
retrieval will be the best for human IVF practice. If one stimulation scheme produces
too many eggs, it often results in hyperstimulation syndrome.
More discrete sequence alterations rely heavily on the use of the PCR, which allows rapid gene amplification and analysis. Moreover, PCR makes it possible to perform genetic testing and mutational analysis with small amounts of DNA extracted from leukocytes or even from single cells, buccal cells, or hair roots. Screening for point mutations can be performed by numerous methods (Table 62-9); most are based on the recognition of mismatches between nucleic acid duplexes, electrophoretic separation of single- or double-stranded DNA, or sequencing of DNA fragments amplified by PCR.
A novel, type 1 ribosome-inactivating protein designated charybdin was
isolated from bulbs of Charybdis maritimaagg. The protein, consisting of a
single polypeptide chain with a molecular mass of 29 kDa, inhibited trans-lation in rabbit reticulocytes with an IC50of 27.2 nm. Plant genomic DNA
extracted from the bulb was amplified by PCR between primers based on
the N-terminal and C-terminal sequence of the protein from dissolved crys-tals.
The naturally synchronous plasmodia of myxomycetes synthesize poly(b-L-malic acid), which carries out cell-speciﬁc functions. In Physarum polycephalum, poly(b-L-malate) [the salt form of poly(b-L-malic acid)] is highly concentrated in the nuclei, repressing DNA synthetic activity of DNA polymerases by the formation of reversible complexes. To test whether this inhibitory activity is cell-cycle-dependent, puriﬁed DNA polymerase a of P.
While the nature and prevalence of defensive medical practices have been widely
debated, most agree that the costs are exorbitant. In fact, some estimates report that
the practice of defensive medicine costs the American health care system in excess of
$100 billion dollars annually, which would account for up to 12% of all health care
Base excision repair (BER), a major pathway for the removal of simple
lesions in DNA, requires the co-ordinated action of several repair and
ancillary proteins, the impairment of which can lead to genetic instability.
We here address the role of poly(ADP-ribose) polymerase-1 (PARP-1) in
BER. Using anin vitro cross-linking assay, we reveal that PARP-1 is
always involved in repair of a uracil-containing oligonucleotide and that it
binds to the damaged DNA during the early stages of repair.
Từ bệnh phẩm là mô sinh thiết của 3 bệnh nhân (BN) được chẩn đoán ung thư vòm họng (UTVH), phát hiện hệ gen EBV bằng phản ứng PCR. Sử dụng kỹ thuật dòng hóa và giải trình tự, toàn bộ chuỗi ARN thông tin đoạn gen LMP-1 của EBV được xác định trong cả 3 mẫu bệnh phẩm nghiên cứu.
Detect Epsstein-Barr virus in patients with nasopharyngeal carcinoma by polymerase chain reaction
Genomic DNA from biopsy tissue of 3 patients with nasopharyngeal carcinoma (NPC) was extracted.
In routine histopathology, most tissues are fixed in formalin and embedded
in paraffin for long-term preservation. DNA can be extracted from these
tissues for subsequent molecular analysis by amplification methods. We
describe herein a protocol for DNA preparation from paraffin-embedded
tissues based on published procedures (1–3). In brief, tissue sections are placed
into microfuge tubes, then deparaffinized with xylene. The xylene is removed
with ethanol washes, and the tissue is treated with proteinase K to make DNA
available for amplification....
Activities completed in the reporting period have included
• seed collections from six Acacia mangium seed production areas (SPAs) in Vietnam
• germination and raising of over 2000 seedlings from the SPAs at RCFTI, Hanoi
• further training of four Vietnamse staff in molecular techniques in Hanoi
• extraction at RCFTI of DNA from 70 maternal parent trees and 700 progeny from the SPAs
• design of field trials for planting of seedlings to allow assessment of inbreeding depression
• arrangements made for training of RCFTI staff in Canberra in October 2001. ...
Cell-free protein synthesis using cell extracts from
Escherichia coli, wheat germ and rabbit reticulocytes
has been used for over 40 years to produce small
amounts of radiolabeled proteins for identification of
gene products and other applications. In the E. coli
system programmed with plasmid DNA, the cell
extract contains or is supplemented with an RNA po-lymerase to transcribe the gene, and the mRNAs are
translated by a complex mixture that contains ribo-somes and a full complement of initiation,...
The gene 4CL1 was isolated from Chinese red pine (Pinus massoniana Lamb) and ligated into vector pPTN289 to perform transformation vector pPTN289-4CL1. This construction was transformed into Agrobacterium tumefaciens strain C58, and then transformed into Chinaberrytree (Melia azedarach L.). The transgenic Chinaberrytree was screened on selection medium (MS + 0.5mg/l 6-BA + 1mg/l vitamine B5 + 30g/l sucrose + 8g/l agar + 500mg/l Cefotaxime + 1mg/l PPT) and then extracted total DNA and tested the existence of interested gene using PCR method. ...
The chromosomal ends ofLeishmania (Leishmania) ama-zonensis contain conserved 5¢-TTAGGG-3¢ telomeric
repeats. Protein complexes that associatein vitro with these
DNA sequences,Leishmania amazonensisG-strand telo-meric protein (LaGT1-3), were identified and characterized
byelectrophoreticmobilityshift assays andUVcross-linking
using protein fractions purified from S100 and nuclear
This experiment examines a human Alu dimorphism at the PV92 locus. A
sample of human cells is obtained by saline mouthwash (alternatively
DNA may be isolated from hair sheaths). DNA is extracted by boiling with
Chelex® resin, which binds contaminating metal ions. Polymerase chain
reaction (PCR) is then used to amplify a chromosome region that contains
the PV92 Alu dimorphism. The Alu insertion allele (+) is 300 nucleotides
longer than the non-insertion allele (–), so the two alleles are readily
separated by agarose gel electrophoresis.
Exoribonuclease II (RNase II), encoded by thernb gene, is a ubiquitous
enzyme that is responsible for 90% of the hydrolytic activity in
Escherichia colicrude extracts. The E. colistrain SK4803, carrying the
mutant allelernb296, has been widely used in the study of the role of
RNase II. We determined the DNA sequence ofrnb296 and cloned this
mutant gene in an expression vector. Only a point mutation in the coding
sequence of the gene was detected, which results in the single substitution
of aspartate 209 for asparagine....