Fluorescence spectroscopy

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  • Ultraviolet Spectroscopy and UV Lasers covers a range of subjects, from ultraviolet (UV) and vacuum ultraviolet (VUV) sources to the latest advances in instrumentation and techniques for absorption, emission, and fluorescence spectroscopy. The book will prove useful to scientists pursuing spectroscopy-related research in fields as varied and diverse as optical physics and engineering, analytical chemistry, biology, and laser technology.

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  • Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Emerging applications of fluorescence spectroscopy in medical microbiology field

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  • The interaction of the adenylate cyclase catalytic domain (AC) of the Bordetella pertussismajor exotoxin with its activator calmodulin (CaM) was studied by time-resolved fluorescence spectroscopy using three fluorescent groups located indifferent regions ofAC: tryptophan residues (W69 and W242), a nucleotide analogue (3¢-anthraniloyl-2¢-deoxyadenosine 5¢-triphosphate, Ant-dATP) and a cysteine-specific probe (acrylodan).

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  • Correct sorting of newly synthesized peroxisomal matrix proteins is dependent on a peroxisomal targeting signal (PTS).So far two PTSs are known.PTS1 consists of a tripeptide that is located at the extremeCterminus ofmatrix proteins and is specifically recognized by the PTS1-receptor Pex5p.We studied Hansenula polymorpha Pex5p (HpPex5p) using fluorescence spectroscopy.The intensity of Trp fluorescence of purified HpPex5p increased by 25% upon shifting the pH frompH 6.0 to pH 7.2.

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  • The health-promoting effects attributed to olive oil, and the development of the olive oil industry have intensified the quest for new information, stimulating wide areas of research. This book is a source of recently accumulated information. It covers a broad range of topics from chemistry, technology, and quality assessment, to bioavailability and function of important molecules, recovery of bioactive compounds, preparation of olive oil-based functional products, and identification of novel pharmacological targets for the prevention and treatment of certain diseases....

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  • Chlorophyll fluorescence represents an intrinsic signal emitted by plants that can be employed to monitor their physiological state including changes of the photosynthetic apparatus, developmental processes of leaves, state of health, stress events, stress tolerance, and also to detect diseases or nutrient deficiency of plants. In particular, the application of laser induced chlorophyll fluorescence spectroscopy has drawn much attention recently owing to the non-invasive and nondestructive nature of the technique (Svanberg, 1995; Lang & Lichtenthaler, 1991; Chappelle et al., 1984)....

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  • Water pollution is a major global problem that requires ongoing evaluation and revision of water resource policy at all levels (from international down to individual aquifers and wells). It has been suggested that it is the leading worldwide cause of deaths and diseases, and that it accounts for the deaths of more than 14,000 people daily.

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  • There have been various comprehensive and stand-alone text books on the introduction to Molecular Photochemistry available in market. A few fantastic books to mention are: ‘Modern Molecular Photochemistry of Organic Molecules’ by N. J. Turro et. al. and ‘Fundamentals of Photochemistry’ by K. K. Rohatgi- Mukherjee. These books along with others give crystal clear idea on the common topics both in pictorial and intuitive terms.

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  • The speculum should be carefully positioned so the entire cervix is seen. If excess mucus or other secretions obscure the cervix, they should be gently removed using a proctoswab without disturbing the epithelium. Small amounts of blood will not interfere with cytologic evaluation, but large amounts, as occurs during menses, preclude cytologic interpretation by conventional Pap smear. This is considerably less of a problem when liquid based cytology is used.

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  • Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging...

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  • The thermal denaturation of endo-b-1,3-glucanase from the hyperthermo-philic microorganismPyrococcus furiosuswas studied by calorimetry. The calorimetric profile revealed two transitions at 109 and 144C, correspond-ing to protein denaturation and complete unfolding, respectively, as shown by circular dichroism and fluorescence spectroscopy data.

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  • This study examines the characteristics of binding of berberine to the human telomeric d[AG3(T2AG3 )3] quadruplex. By employing UV-visible spectroscopy, fluorescence spectroscopy and isothermal titration calorime-try, we found that the binding affinity of berberine to the human telomeric quadruplex is 10 6 .

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  • A small amino acid sequence (LWYIK) inside the HIV-1 gp41 ectodomain membrane proximal region (MPR) is commonly referred to as a choles-terol-binding domain. To further study this unique and peculiar property we have used fluorescence spectroscopy techniques to unravel the mem-brane interaction properties of three MPR-derived synthetic peptides: the membrane proximal region peptide-complete (MPRP-C) which corresponds to the complete MPR;

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  • We have used fluorescence quench titrations, EPR spectroscopy and steady-state kinetics to study the effects of site-directed mutants of FrdB, FrdC and FrdD on the proximal menaquinol (MQH2) binding site (QP)of Escherichia colifumarate reductase (FrdABCD) in cytoplasmic membrane preparations. Fluorescence quench (FQ) titrations with the fluorophore and MQH2 analog 2-n-heptyl-4-hydroxyquinoline-N-oxide (HOQNO) indi-cate that the QPsite is defined by residues from FrdB, FrdC and FrdD. In FQ titrations, wild-type FrdABCD binds HOQNO with an apparentKdof 2.

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  • The extreme thermal stabilization achieved by the introduction of a disul-fide bond (G8C⁄N60C) into the cysteine-free wild-type-like mutant (pWT) of the neutral protease fromBacillus stearothermophilus[Mansfeld J, Vri-end G, Dijkstra BW, Veltman OR, Van den Burg B, Venema G, Ulbrich-Hofmann R & Eijsink VG (1997)J Biol Chem 272, 11152–11156] was attributed to the fixation of the loop region 56–69.

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  • Depth of bilayer penetration and effects on lipid mobility conferred by themembrane-active peptidesmagainin, melit-tin, and a hydrophobic helical sequence KKA(LA)7KK (denoted KAL), were investigated by colorimetric and time-resolved fluorescence techniques in biomimetic phos-pholipid/poly(diacetylene)vesicles. The experiments dem-onstrated that the extent of bilayer permeation and peptide localization within the membrane was dependent upon the bilayer composition, and that distinct dynamic modifica-tions were induced by each peptide within the head-group environment of the phospholipids....

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  • The structural features of the hyperthermophilic endo-b-1,3-glucanase from Pyrococcus furiosuswere studied using circular dichroism, steady-state and time-resolved fluorescence spectroscopy and anisotropy. Upon heat and chemical treatment the folded and denatured states of the protein were characterized by distinguishable spectral profiles that identified a number of conformational states.

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  • The interaction of heat shock protein with molecular mass 25 kDa (HSP25) and its point mutants S77D + S81D (2D mutant) and S15D + S77D + S81D (3D mutant) with intact and thermally denatured actinwas analyzedbymeans of fluorescence spectroscopy and ultracentrifugation. Wild typeHSP25 did not affect the polymerization of intact actin. The HSP25 3D mutant decreased the initial rate without affecting the maximal extent of intact actin polymerization. G-actin heated at 40–45C was partially denatured, but retained its ability to polymerize....

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  • he characterization of individual molecules has been a scientifically attractive and challenging task for decades, and remains so today. New technological developments have facilitated great progress in our understanding of the structure and behavior of single atoms and molecules in various environments.

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  • Ascorbate oxidase (AAO) is a large, multidomain, dimeric protein whose folding⁄unfolding pathway is characterized by a complex, multistep pro-cess. Here we used fluorescence correlation spectroscopy to demonstrate the formation of partially folded monomers by pH-induced full dissociation into subunits.

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