The dye-decolorizing peroxidase (DyP)-type peroxidase family is a unique
heme peroxidase family. The primary and tertiary structures of this family
are obviously different from those of other heme peroxidases. However,
the details of the structure–function relationships of this family remain
The chemical sensor plays an essential role in the fields of environmental conservation and monitoring, disaster and disease prevention, and industrial analysis. A typical chemical sensor is a device that transforms chemical information in a selective and reversible way, ranging from the concentration of a specific sample component to total composition analysis, into an analytically useful signal.
In this study we investigate the active-site structure and the
catalytic mechanism of clostripain by using a combination
of three separate techniques: affinity labelling, site-directed
mutagenesis and molecular modelling. A benzamidinyl-diazo dichlorotriazine dye (BDD) was shown to act as an
efficient active site-directed affinity label for Clostridium
histolyticumclostripain. The enzyme, upon incubation with
BDD in 0.1MHepes/NaOHbuffer pH 7.6, exhibits a time-dependent loss of activity.
3,3¢-Dipropyl-2,2¢-thiadicarbocyanine iodide [DiS-C3
often used as a tracer dye to assess the mitochondrial mem-brane potential, was investigated in detail regarding its
effects on the structure and function of isolated mitochon-dria. As reported previously, DiS-C3(5) had an inhibitory
effect on NADH-driven mitochondrial electron transfer.
On the contrary, in the presence of inorganic phosphate,
DiS-C3(5) showed dose-dependent biphasic effects on mito-chondria energized by succinate. At higher concentrations,
such as 50lM,DiS-C3(5) acceleratedmitochondrial oxygen
Phenolic compounds represent a large group of molecules with a variety
of functions in plant growth, development, and defense. Phenolic
compounds include signaling molecules, pigments and flavors that can
attract or repel, as well as compounds that can protect the plant against
insects, fungi, bacteria, and viruses. Most phenolic compounds are present
as esters or glycosides rather than as free compounds. Tannins and lignin are
oscillation occurs in various cell types to regulate cell-ular functions. However, the mechanism for synchronization of Ca
increases between cells remains unclear. Recently, synchronous oscillatory
changes in the membrane potential of internal Ca
stores were recorded
using an organelle-specific voltage-sensitive dye [Yamashita et al. (2006)
FEBS J273, 3585–3597], and an electrical coupling model of the synchro-nization of store potentials and Ca
releases has been proposed [Yamash-ita (2006) FEBS Lett580, 4979–4983]....