Genome walking is a molecular procedure for the direct identification of
nucleotide sequences from purified genomes. The only requirement is the
availability of a known nucleotide sequence from which to start.
This paper was originally presented as the first in the Sol Plaatjie Lecture Series on Africa, jointly hosted by the Ministry of Education and the African Human Genome Initiative in November 2002. The author Himla Soodyall addresses the contribution towards the generation of knowledge concerning the evolutionary history of mankind made by genetic approaches to anthropological questions. She examines the use and relevance of genetic data as another ’tool’ in the reconstruction of our history. About the Author/sProfessor Himla Soodyall obtained a B.Sc (Hons) degree at the University...
The car-sharing concept originated in Switzerland in 1987, and one of the largest car-sharing opera-
tions in the world is still run by Mobility Switzerland, in close partnership with the Swiss Federal
Railway. It runs a fleet of 1,650 cars in more than 930 locations, and has enrolled more than 58,000
members. As well as its size, the Swiss program has enjoyed enormous success in influencing travel
behavior. Surveys show that members who gave up their car after joining the car-sharing program
increased their transit usage by 35%, from 3,560 miles per year to 4,810 miles per year.
We describe a strategy for systematic amplification of chitin
synthase genes (chs) in the filamentous ascomycetes plant-pathogenBotrytis cinereausing PCR with multiple degen-erate primers designed on specific and conserved sequence
motifs. Eight distinctchsgeneswere isolated, namedBcchs I,
II, IIIa, IIIb, IV, V, VIandVII. They probably constitute
the entire chsmultigenic family of this fungus, as revealed
by careful analysis of six euascomycetes genomes.
Discovering what lies behind a hill or beyond a neighborhood can be as
simple as taking a short walk. But curiosity and the urge to make new dis-
coveries usually require people to undertake journeys much more adven-
turesome than a short walk, and scientists oft en study realms far removed
from everyday observation—sometimes even beyond the present means
of travel or vision.
An NADH oxidase (NOX) was cloned from the genome ofThermococ-cus profundus (NOXtp) by genome walking, and the encoded protein was
purified to homogeneity after expression in Escherichia coli.Subsequent
analyses showed that it is an FAD-containing protein with a subunit
molecular mass of 49 kDa that exists as a hexamer with a native molecular
mass of 300 kDa.
We have the power to explore the planets and walk the moon. We have
the power to coax green from the deserts. We have the power to map
the human genome.
Yet in the year 2005, in millions of communities in every corner of the globe,
people are suffering because those with political power have failed to meet
their most basic responsibilities. That failure is seen in the crisis of local health
systems that do not work, that exclude the poor, abuse and marginalize women,
sow distrust and feed corruption.