DNA fragmentation is a hallmark of apoptosis that occurs in a variety of
cell types; however, it remains unclear whether caspase-3 is required for its
induction. To investigate this, we produced caspase-3 knockout Chinese
hamster ovary (CHO)-K1 cells and examined the effects of gene knockout
and treatment with caspase-3 inhibitors.
GM-CSF is one of several naturally occurring glycoproteins
that regulate leukocyte production, migration and function.
It has been produced in different cell types, with different
properties that depend on the production process used. The
purpose of this work was to characterize the recombinant
human GM-CSF from an engineered Chinese hamster
ovary cell line grown in suspension and as adherent culture
for the identification of the glycosylation sites and the defi-nition of the glycosidic moiety, including the degree of site
The structure of post-translational modiﬁcations of human heparin cofactor II isolated from human serum and from recombinant Chinese hamster ovary cells and their eﬀects on heparin binding have been characterized. Oligosaccharide chains were found attached to all three potential N-glycosylation sites in both protein preparations. The carbohydrate structures of heparin cofactor II circulating in blood are complex-type diantennary and triantennary chains in a ratio of 6 : 1 with the galactose being 90% sialylated with a2 ﬁ 6 linked N-acetylneuraminic acid....
Apurinic⁄apyrimidinic endonuclease (APE), an essential DNA repair
enzyme, initiates the base excision repair pathway by creating a nick 5¢ to
an abasic site in double-stranded DNA. Although the Chinese hamster
ovary cells remain an important model for DNA repair studies, the Chinese
hamster APE (chAPE1) has not been studied in vitro in respect to its
Modern biotechnology is often associated with the use of genetically altered microorganisms such as E. coli or yeast for the production of substances like synthetic insulin or antibiotics. It can also refer to transgenic animals or transgenic plants, such as Bt corn. Genetically altered mammalian cells, such as Chinese Hamster Ovary cells (CHO), are also used to manufacture certain pharmaceuticals. Another promising new biotechnology application is the development of plant-made pharmaceuticals.
The cloned guinea-pig Y2 neuropeptide Y (NPY) receptors expressed in Chinese hamster ovary (CHO) cells, as well as the Y2 receptors natively expressed in rat forebrain, are distributed in two populations. A smaller population that is readily accessed by agonist peptides on the surface of intact cells constitutes less than 30% of Y2 receptors detected in particulates after cell homogenization. A much larger fraction of cell surface Y2 sites can be activated by sulfhydryl modiﬁers.