NFκB plays an important role in the immune system in all organisms. In this study, physicochemical properties and modelling of finfish NFκBIα protein was analysed using in silico approach. Finfish species: guppy (Poecilia reticulate), Bicolor damselfish (Stegastes partitus), Channel catfish (Ictalurus punctatus), Coelacanth (Latimeria chalumnae), and Australian ghostshark (Callorhinchus milii) were used in this study.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Binding site of ABC transporter homology models confirmed by ABCB1 crystal structure...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Theoretical study of the Usutu virus helicase 3D structure, by means of computer-aided homology modelling
An homology model of human P-glycoprotein, based on the X-ray struc-ture of the recently resolved mouse P-glycoprotein, is presented. The
model corresponds to the inward-facing conformation competent for drug
binding. From the model, the residues involved in the protein-binding cav-ity are identified and compared with those in the outward-facing confor-mation of human P-glycoprotein developed previously based on the
In the Biomedical Applications of Biophysics, Volume 3 of the Handbook of Modern Biophysics,
the authors have added to the topics introduced in Volume 1, Fundamental Concepts in Biophysics.
These additional topics help trace the broad field of biophysics.
Patrice Koehl starts the book with an introduction to protein structure prediction based on
energetics, homology modeling, and ab-initio calculations. Dickey and Faller follow with a
“how-to approach” to model biomembranes.
The plant enzyme phenylalanine ammonia-lyase (PAL, EC 22.214.171.124) shows homology to histidine ammonia-lyase (HAL) whose structure has been solved by X-ray crystallography. Based on amino-acid sequence alignment of the two enzymes, mutagenesis was performed on amino-acid residues that were identical or similar to the active site residues in HAL to gain insight into the importance of this residues in PAL for substrate binding or catalysis. We mutated the following amino-acid residues: S203, R354, Y110, Y351, N260, Q348, F400, Q488 and L138....
Homology modeling in combination with transmembrane
topology predictions are used to build the atomic model of
Neurospora crassaplasmamembrane H
-ATPase, using as
template the 2.6 A
crystal structure of rabbit sarcoplasmic
-ATPase [Toyoshima, C., Nakasako, M.,
Nomura, H. & Ogawa, H. (2000)Nature405, 647–655].
Tham khảo luận văn - đề án 'báo cáo y học: " the role of qa-2, the functional homolog of hla-g, in a behcet's disease-like mouse model induced by the herpes virus simplex"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả
DPP IV has been attributed a large array of functions, some of which are mediated by
its exopeptidase activity. Although it only removes two amino acid residues at the N-terminus
of the peptide, this cleavage can inactivate or modify the activity of regulatory peptides, peptide
hormones, chemokines and neuropeptides. Several excellent DPP IV substrates with high
specificity constants were identified by the in vitro kinetic study of the truncation of bioactive
peptides by DPP IV. In vivo studies e.g.
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex...
The thermodynamic stability of family 16 endo-b-1,3-glucanase
(EC 126.96.36.199) from the hyperthermophilic archaeon Pyrococcus furiosusis
decreased upon single (D287A, E53A) and double (E53A⁄D287A) muta-tion of Asp287 and Glu53. In accordance with the homology model predic-tion, both carboxylic acids are involved in the composition of a calcium
binding site, as shown by titration of the wild-type and the variant proteins
with a chromophoric chelator.
Aniline dioxygenase is a multicomponent Rieske nonheme-iron dioxygenase
enzyme isolated from Acinetobactersp. strain YAA. Saturation mutagen-esis of the substrate-binding pocket residues, which were identified using a
homology model of theasubunit of the terminal dioxygenase (AtdA3), was
used to probe the molecular determinants of AtdA substrate specificity.
A recombinant lipase cloned from Pseudomonas fragi strain IFO 3458 (PFL) was found to retain signiﬁcant activity at low temperature. In an attempt to elucidate the structural basis of this behaviour, a model of its three-dimensional structure was built by homology and compared with homologous mesophilic lipases, i.e. the Pseudomonas aeruginosa lipase (45% sequence identity) and Burkholderia cepacia lipase (38%).
Ion mobility MS was employed to study the structure of thebB2B3-crystal-lin heterodimer following its detection by ESI-TOF MS. The results dem-onstrate that the heterodimer has a similar cross-section (3 165 A˚
structure to the bB2B2-crystallin homodimer.
H2O2 induces apoptosis in variety of cells; however, the sensitivities of
testicular germ cells to H2O2are not known. In the present study, H2O2
concentrations in the range 1–10lm, was found to induce apoptosis in
testicular germ cells in vitro.
We report the characterization of the pyrimidine-specific ribonucleoside
hydrolase from the hyperthermophilic archaeon Sulfolobus solfataricus
(SsCU-NH). The gene SSO0505 encoding SsCU-NH was cloned and
expressed in Escherichia coliand the recombinant protein was purified to
FKBP22 from a psychrotrophic bacteriumShewanellasp. SIB1, is a dimer-ic protein with peptidyl prolyl cis-transisomerase (PPIase) activity. Accord-ing to homology modeling, it consists of an N-terminal domain, which is
involved in dimerization of the protein, and a C-terminal catalytic domain.
A longa3 helix spans these domains. An N-domain with the entirea3 helix
) and a C-domain with the entire a3 helix (C-domain
overproduced inEscherichia coliin a His-tagged form, purified, and their
biochemical properties were compared with those of the intact protein.
The substrate specificity of the nuclear inclusion protein a (NIa) proteolytic
enzymes from two potyviruses, the tobacco etch virus (TEV) and tobacco
vein mottling virus (TVMV), was compared using oligopeptide substrates.
Mutations were introduced into TEV protease in an effort to identify key
determinants of substrate specificity. The specificity of the mutant enzymes
was assessed by using peptides with complementary substitutions. The crys-tal structure of TEV protease and a homology model of TVMV protease
were used to interpret the kinetic data....
Geodin is a protein encoded by a sponge gene homologous to genes from
the bc-crystallins superfamily. The interest for this crystallin-type protein
stems from the phylogenesis of porifera, commonly called sponges, the
earliest divergence event in the history of metazoans. Here we report the
preparation of geodin as a recombinant protein fromEscherichia coli, its
characterization through physico-chemical analyses, and a model of its 3D
structure based on homology modelling.
Fructose 2,6-bisphosphate is a potent allosteric activator of trypanosomatid
pyruvate kinase and thus represents an important regulator of energy meta-bolism in these protozoan parasites. A 6-phosphofructo-2-kinase, respon-sible for the synthesis of this regulator, was highly purified from the
bloodstream form ofTrypanosoma bruceiand kinetically characterized. By
searching trypanosomatid genome databases, four genes encoding proteins
homologous to the mammalian bifunctional enzyme 6-phosphofructo-2-kinase⁄fructose-2,6-bisphosphatase (PFK-2⁄FBPase-2) were found for both