Hpv dna test

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  • Those of us involved in the healthcare of women have seen a remarkable transformation in screening techniques for cervical cancer and its precursors since the mid 1990s.  The staid old Pap smear technique of scraping cells from the cervix with a wooden spatula and cotton-tipped applicator and smearing them onto a glass slide is a thing of the past in most practices. We now use plastic collection devices to transfer cells from the cervix into a preservative which is sent to the lab for liquidbased cytology and reflex human papillomavirus (HPV) testing.

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  • In contrast, only three percent of women who had a negative HPV DNA test for 13 high risk types developed CIN3+ within the timeframe of the study, indicating that frequent screening of these women may be unnecessary. The researchers found that although infection with some types, such as HPV 53, 56, 59, and 68, were prone to persist for 2 years, they did not lead to lesions during the entire follow-up period. A study in Italy18 investigated the efficacy of HPV DNA testing for CIN and cervical cancers and supported the use of stand-alone HPV DNA testing as...

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  • Investigators continue to evaluate screening technologies in meta-analyses, randomized clinical trials, and cervical cancer prevention programs. The new evidence adds support for the use of HPV DNA testing as the primary technology for cervical cancer screening, both in high-resource areas and in developing countries. 6–8 Despite this evidence, significant barriers to widespread use of HPV DNA testing stand in the way, both in high- and low-resource areas.

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  • Screening for precancerous lesions can be done in several ways including, cervical cytology (Pap tests), visual inspection of the cervix with acetic acid [VIA] or testing for HPV DNA. Each of these methods has specific advantages, disadvantages and health systems requirements that countries should consider when planning screening programmes (See Annex 1).

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  • A recent report 10 from mainland China concluded that HPV DNA testing is highly sensitive and moderately specific for cervical intraepithelial neoplasia 3 or worse (CIN3+; CIN3 is the usual immediate precursor to invasive cervical cancer), with consistent results across study sites and age groups. This paper reviewed reports of more than 30,000 women from population-based screening studies done between 1999 and 2008, with the aim of assessing whether HPV DNA testing could be applied to cervical cancer screening programs in China.

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