Glutathione (GSH) is an intracellular antioxidant synthesized from gluta-mate, cysteine and glycine. The human erythrocyte (red blood cell, RBC)
requires a continuous supply of glutamate to prevent the limitation of GSH
synthesis in the presence of sufficient cysteine, but the RBC membrane is
almost impermeable to glutamate.
The nature of cGMP transport in human erythrocytes, its
relationship to glutathione conjugate transport, and pos-sible mediation by multidrug resistance-associated proteins
(MRPs) have been investigated. MRP1, MRP4 and MRP5
are detected in immunoblotting studies with erythrocytes.
MRP1and MRP5 are also detected in multidrug resistant
COR-L23/R and MOR/R cells but at greatly reduced levels
in the parent, drug sensitive COR-L23/P cells. MRP4 is
detected in MOR/R but not COR-L23/R cells.
Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study...
Alkyl nitrites are highly volatile organic solvents of aliphatic alcohol esters of nitrites . Amyl nitritea, butyl nitrite and isobutyl nitrite are the representative alkyl nitrites; their boiling points are 98, 78 and 67 °C, respectively. Amyl nitrite is being widely used as a detoxicant for cyanide poisoning, because alkyl nitrites oxidize hemoglobin in erythrocytes to yield methemogloblin, which is bound with cyanide to inactivate it .
This article is devoted to the study of redundancy and yield of salvage
pathways in human erythrocytes. These cells are not able to synthesize
ATPde novo. However, the salvage (recycling) of certain nucleosides or
bases to give nucleotide triphosphates is operative. As the salvage pathways
use enzymes consuming ATP as well as enzymes producing ATP, it is not
easy to see whether a net synthesis of ATP is possible. As for pathways
using adenosine, a straightforward assumption is that these pathways start
with adenosine kinase....
Dendrimeric peptides selective for microbial surfaces have been developed to achieve broad antimicrobial activity and low hemolytic activity to human erythrocytes. The dendrimeric core is an asymmetric lysine branching tethered with two to eight copies of a tetrapeptide (R4) or an octapeptide (R8). The R4 tetrapeptide (RLYR) contains a putative microbial surface recognition BHHB motif (B ¼ basic, H ¼ hydrophobic amino acid) found in protegrins and tachyplesins whereas the octapeptide R8 (RLYRKVYG) consists of an R4 and a degenerated R4 repeat....
cGMP secretion from cells can be mediated by ATP-binding cassette
(ABC) transporters ABCC4, ABCC5, and ABCC11. Indirect evidence sug-gests that ABCC4 and ABCC5 contribute to cGMP transport by erythro-cytes. We have re-investigated the issue using erythrocytes from wild-type
and transporter knockout mice.
Hemoglobin is the most prominent protein in blood, transporting O2 and
facilitating reactive oxygen and nitrogen species detoxification. Hemoglobin
metabolism leads to the release of extra-erythrocytic hemoglobin, with
potentially severe consequences for health. Extra-erythrocytic hemoglobin
is complexed to haptoglobin for clearance by tissue macrophages.
Malaria, caused by four species of Plasmodium, of which Plasmodium falciparum is the most
dangerous, remains the world's most devastating human parasitic infection. This chapter deals with
the properties and uses of important drugs used to treat and prevent this infection. Highly effective
agents that act against asexual erythrocytic stages of malarial parasites responsible for clinical
attacks include chloroquine, quinine, quinidine, mefloquine, atovaquone, and the artemisinin