Immobilized enzymes

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  • We live in the age of biology—the human and many other organisms’ genomes have been sequenced and we are starting to understand the function of the metabolic machinery responsible for life on our planet. Thousands of new genes have been discovered, many of these coding for enzymes of yet unknown function. Understanding the kinetic behavior of an enzyme provides clues to its possible physiological role. From a biotechnological point of view, knowledge of the catalytic properties of an enzyme is required for the design of immobilized enzyme-based industrial processes.

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  • Về công nghệ enzym/protein người ta đã sử dụng thành công kỹ thuật enzym bất động (immobilized ezyme) hoặc tế bào bất động (immobilized cell) để sản xuất ở quy mô công nghiệp các sản phẩm được tạo thành nhờ hoạt động xúc tác của enzym. Nhờ sử dụng công nghệ gen người ta có thể tạo ra khả năng sản sinh một enzym mới nhờ vi khuẩn hoặc nấm men hoặc là nâng cao thêm lên nhiều lần hoạt tính sản sinh enzym của chúng.

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  • Biopolymers are polymers produced by living organisms. Cellulose, starch, chitin, proteins, peptides, DNA and RNA are all examples of biopolymers. This book comprehensively reviews and compiles information on biopolymers in 30 chapters. The book covers occurrence, synthesis, isolation and production, properties and applications, modification, and the relevant analysis methods to reveal the structures and properties of some biopolymers.

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  • Cố định vi khuẩn bằng hydroxide kim loại làm tăng độ nhạy của phương pháp PCR khi phát hiện vi khuẩn trong thực phẩm. Vi khuẩn ở nồng độ thấp không qua nuôi dưỡng có thể phát hiện được bằng phương pháp PCR nhờ cô đặc vi khuẩn từ thực phẩm bằng hydroxide kim loại. Enzyme DNase I được sử dụng để loại bỏ DNA từ vi khuẩn sống, do đó phương pháp PCR có thể dùng để phát hiện vi khuẩn sống.

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  • The topoisomerase I (TopI) reaction intermediate consists of an enzyme covalently linked to a nicked DNA molecule, known as a TopI-DNA complex, that can be trapped by inhibitors and results in failure of re-ligation. Attempts at new derivative designs for TopI inhibition are enthusiastically being pursued, and TopI inhibitors were developed for a variety of applications. Surface plasmon resonance (SPR) was recently used in TopI-inhibition studies.

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  • Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effect of substrate (ZnO) morphology on enzyme immobilization and its catalytic activity

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  • Possible binding proteins of CP12 in a green alga,Chlamydomonas rein-hardtii, were investigated. We covalently immobilized CP12 on a resin and then used it to trap CP12 partners. Thus, we found an association between CP12 and phosphoribulokinase (EC 2.7.1.19), glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.13) and aldolase.

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  • Snake venoms are cocktails of enzymes and non-enzymatic proteins used for both the immobilization and digestion of prey. The most common snake venom enzymes include acetylcholinesterases, L-amino acid oxidases, serine proteinases, metalloproteinases and phospholipases A2

    pdf33p cosis54 04-01-2013 19 3   Download

  • Cyclodextrin glycosyltransferase catalyzes the formation of a mixture of cyclodextrins from starch by an intramolecular transglycosylation reaction. To manipulate the product specificity of thePaenibacillussp. A11 and Bacillus maceranscyclodextrin glycosyltransferases towards the preferential formation of c-cyclodextrin (CD8), crosslinked imprinted proteins of both cyclodextrin glycosyltransferases were prepared by applying enzyme imprinting and immobilization methodologies.

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  • Carboxypeptidases were purified fromguts of larvae of corn earworm (Helicoverpa armigera), a lepidopteran crop pest, by affinity chromatography on immobilized potato carb-oxypeptidase inhibitor, and characterized by N-terminal sequencing. A larval gut cDNA library was screened using probes based on these protein sequences. cDNA HaCA42 encoded a carboxypeptidase with sequence similarity to enzymesofclan MC [Barrett,A.J.,Rawlings,N. D.& Woessner, J. F. (1998)Handbook of Proteolytic Enzymes. Academic Press, London.], but with a novel predicted spe-cificity towards C-terminal acidic residues....

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