Research on ion channels has exploded in the last few decades and it is now
clear that ion channels play essential roles in cell biology and physiology, with
their dysfunction being the root cause of many human diseases. Understanding
human biology in the post-genome sequencing era requires that the function of
the protein products encoded by these recently sequenced genes be quantified.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells...
(BQ) Part 2 book "Cellular physiology and neurophysiology" presents the following contents: Electrical consequences of ionic gradients, ion channels, passive electrical properties of membranes, generation and propagation of the action potential, ion channel diversity, passive solute transport, passive solute transport.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: dentification of the prokaryotic ligand-gated ion channels and their implications for the mechanisms and origins of animal Cys-loop ion channels...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Alternative ion channel splicing in mesial temporal lobe epilepsy and Alzheimer's disease...
The biological ion channel gramicidin A (gA) was modified by synthetic
means to obtain the tail-to-tail linked asymmetric gA-derived dimer com-pound3. Single-channel current measurements for 3in planar lipid bilayers
exhibit an Eisenman I ion selectivity for alkali cations. The structural
asymmetry does not lead to an observable functional asymmetry. The
structure of 3in solution without and with Cs cations was investigated by
H-NMR spectroscopy. In CDCl3
⁄CD3OH (1 : 1, v⁄v),3forms a mixture
The structure of CLIC4, a member of the CLIC family of putative intracel-lular chloride ion channel proteins, has been determined at 1.8 A
by X-ray crystallography. The protein is monomeric and it is structurally
similar to CLIC1, belonging to the GST fold class. Differences between the
structures of CLIC1 and CLIC4 are localized to helix 2 in the glutaredoxin-like N-terminal domain, which has previously been shown to undergo a dra-matic structural change in CLIC1 upon oxidation.
Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ chanThe nuclear geneMRS2in Saccharomyces cerevisiaeencodes an integral
protein (Mrs2p) of the inner mitochondrial membrane. It forms an ion
channel mediating influx of Mg
into mitochondria. Orthologues of
Mrs2p have been shown to exist in other lower eukaryotes, in vertebrates
and in plants.nel protein of Saccharomyces cerevisiae
` Julian Weghuber, Frank Dieterich, Elisabeth M. Froschauer, Sona Svidova and Rudolf J. Schweyen
Protein–protein interactions are critical for protein trafficking, localization
and the regulation of ion channels. Thehuman ether-a-go-go-related gene
(herg) encodes the a-subunit of the potassium channel underlying the rapid
component of the cardiac delayed rectifier current.
Endovanilloids are defined as endogenous ligands of the
transient receptor potential vanilloid type 1 (TRPV1)
protein,a nonselective cation channel that belongs to the
large family of TRP ion channels,and is activated by
the pungent ingredient of hot chilli peppers,capsaicin.
TRPV1 is expressed in some nociceptor efferent neurons,
where it acts as a molecular sensor of noxious heat and
low pH. However,the presence of these channels in var-ious regions of the central nervous system,where they are
not likely to be targeted by these noxious stimuli,suggests
the existence of endovanilloids.
KcsA, a potassium channel fromStreptomyces lividans, was the first ion
channel to have its transmembrane domain structure determined by crystal-lography. Previously we have shown that its C-terminal cytoplasmic
domain is crucial for the thermostability and the expression of the channel.
Expression was almost abolished in its absence, but could be rescued by
the presence of an artificial left-handed coiled coil tetramerization domain
The voltage-gated potassium channel Kv4.3 was coexpressed with itsb-sub-unit Kv channel-interacting protein 2 and the angiotensin type 1 receptor in
HEK-293 cells. Proteomic analysis of proteins coimmunoprecipitated with
Kv4.3 revealed that Kv4.3 is associated with Rap guanine nucleotide
exchange factors MR-GEF and EPAC-1.
Chloride intracellular channels (CLICs) are soluble, signal peptide-less pro-teins that are distantly related to W-type glutathione-S-transferases.
Although some CLICs bypass the classical secretory pathway and auto-insert into cell membranes to form ion channels, their cellular roles remain
Nicotinic acetylcholine receptors, or nAChRs, are cholinergic receptors that form ligand-gated ion channels in the plasma membranes of certain neurons and on the postsynaptic side of the neuromuscular junction . Being ionotropic receptors, nAChRs are directly linked to an ion channel and do not make use of a second messenger as metabotropic receptors do.
The historical route to present-day patch clamping started with the
scientific recognition that electrical phenomena are part of animal physiology.
This bioelectricity was demonstrated in the nineteenth century in
frogs, where muscle movements could be evoked by applying electrical
stimuli to the animal. The recording of inherent electrical activity can be
charted by the development of increasingly sophisticated electrodes.
Volume 3 of the book series Biomathematical and Biomechanical Modeling of
the Circulatory and Ventilatory Systems aims at presenting major sets of signaling
receptors mainly located at the plasma membrane, 1 in a modeling framework rather
than biological perspective. Collecting signaling effectors, their main interactions,
and major properties are the first tasks required for any modeling of cell signaling
The major objective is to comprehend the complexity of natural phenomena to
model these events. In other words, to yield the maximal amount of known information
Protein kinase Ce is expressed at higher levels in the brain compared to
other tissues such as the heart and kidney, suggesting that it plays an
important role in the nervous system. Several neural functions of PKCe,
including neurotransmitter release and ion channel regulation, have been
identified using PKCe knockout mice.
Puroindoline-a (PIN-a) anda1-purothionin (a1-PTH), isolated from wheat
endosperm ofTriticum aestivumsp., have been suggested to play a role in
plant defence mechanisms against phytopathogenic organisms. We investi-gated their ability to form pores when incorporated into giant liposomes
using the patch-clamp technique.
channels are an octameric assembly of
two proteins, a sulfonylurea receptor (SUR1)and an ion
conducting subunit (Kir 6.0). We have examined the role of
the C-terminus of SUR1 by expressing a series of truncation
mutants together with Kir6.2 stably in HEK293 cells. Bio-chemical analyses using coimmunoprecipitation indicate
that SUR1 deletionmutants andKir6.2 assemble and that a
SUR1 deletion mutant binds glibenclamide with high affin-ity.