Biology is chemistry on an impressive scale. It is a product of evolution, the
outcome of countless random experiments, resulting in the exquisite
complexity of the biological world of which we are a part. Setting aside any
philosophical considerations, living organisms - including ourselves - are
simply nothing more than wet, floppy bags of chemistry: complicated
mixtures of molecules interacting in a multitude of ways. All this takes place
mainly in ...
We havemade a comprehensive and quantitative analysis of
the lipid composition of caveolae from primary rat fat cells
and compared the composition of plasma membrane inside
and outside caveolae. We isolated caveolae from purified
to disrupt the membrane, or extraction with nonionic
detergent, followed by density gradient ultracentrifugation.
The carbonate-isolated caveolae fraction was further
immunopurified using caveolin antibodies.
Leukocytes and other cells show an enhanced intensity of
mobile lipid in their
H NMR spectra under a variety of
conditions.Such conditions include stimulation, which has
recently been shown to involve detergent-resistant, plasma
membranedomains (DRMs)oftencalled lipidrafts.As there
ismuchspeculationsurrounding theoriginof cellularNMR-visible lipid, we analysed subcellular fractions, including
DRMs, by NMR spectroscopy.
Using the broadest possible definition, a lipid (Greek
) may be defined as a compound
of low or intermediate molecular weight (5000), a substantial proportion of which is made up of
hydrocarbons. Included are diverse compounds such as fatty acids; soaps; detergents; steroids;
mono-, di-, and triacylglycerols; and more complex compounds such as phospholipids, sphingolipids,
glycolipids, and lipopolysaccharides.