Mass spectrometer

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  • This studywas designed to yield data on the supramolecular organization of the phycobilisome apparatus from Synechocystis, and the possible effects of environmental stress on this arrangement. Phycobilisomes were dissociated ina low ionic strength solutionandaquantitative estimation of the protein components present in each subcomplex was obtained using liquid chromatography coupled on-line with a mass spectrometer equipped with an electrospray ion source (ESI-MS).

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  • A great deal has transpired since the final reprint of the previous edition of Fundamentals of Vacuum Technology appeared in 1987. LEYBOLD has in the meantime introduced a number of new developments in the field. These include the dry-running ALL×ex chemicals pump, the COOLVAC-FIRST cryopump systems with quick regeneration feature, turbomolecular pumps with magnetic bearings, the A-Series vacuum gauges, the TRANSPECTOR and XPR mass spectrometer transmitters, leak detectors in the UL series, and the ECOTEC 500 leak detector for refrigerants and many other gases.

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  • ESI greatly enhanced the ability to characterize protein and peptides by MS.  ESI requires the sample of interest to be in solution- flow in to the ionization source region.  To ionize the sample, high voltage is applied, result in the sample becoming positively or negatively charged; however, positive ionization is used primarily in the analysis proteins and peptides.  As it exits the spray tip, the solution produces submicrometer-sized droplets containing both solute and analyte ions. The sample is then desorbed of solute prior to entering the analyzer region....

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  • An inert gas is bubbled through a 5 mL water sample contained in a specially-designed purging chamber at ambient temperature. The purgeables are efficiently transferred from the aqueous phase to the vapor phase. The vapor is swept through a sorbent trap where the purgeables are trapped. After purging is completed, the trap is heated and backflushed with the inert gas to desorb the purgeables onto a gas chromatographic column. The gas chromatograph is temperature programmed to separate the purgeables which are then detected with a mass spectrometer....

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  • Mass spectrometric studies on the N-linked glycans of aminopeptidase 1 from Manduca sextahave revealed unusual structures not previously observed on any insect glycoprotein. Structure elucidation of these oligosaccha-rides was carried out by high-energy collision-induced dissociation (CID) using a matrix-assisted laser desorp-tion/ionization time-of-flight/time-of-flight (MALDI-TOF/ TOF) tandem mass spectrometer.

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  • For the characterization of protein sequences and post-translational modifi-cations by MS, the ‘top-down’ proteomics approach utilizes molecular and fragment ion mass data obtained by ionizing and dissociating a protein in the mass spectrometer.

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  • Peptide and protein sequence analysis using a combination of gas-phase ion–ion chemistry and tandem MS is described. Samples are converted to multiply charged ions by ESI and then allowed to react with fluoranthene radical anions in a quadrupole linear ion trap mass spectrometer.

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