The aim of this study was to assess the effects of Pomelo (Citrus grandis (L.) Osbeck) peel extracts (CGE) on activity of lipid-carbohydrate metabolic enzymes such as Carnitine pamitoyl-transferase (CPT), lipase, hexokinase, glucose 6-phosphatase and blood lipid glucose parameters in experimental obese and diabetic mice. The results showed that, in the experimental obese mice treated daily, orally with CGE at dose of 1200 mg/body weight for three weeks,
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Xenobiotic metabolizing enzyme gene polymorphisms predict response to lung volume reduction surgery...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Polymorphisms of two histamine-metabolizing enzymes genes and childhood allergic asthma: a case control study...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Drug metabolizing enzyme activities versus genetic variances for drug of clinical pharmacogenomic relevance...
A fatty-acid-metabolizing enzyme fromArabidopsis thaliana, CYP94C1,
belonging to the cytochrome P450 family was cloned and characterized.
CYP94C1 was heterologously expressed in aSaccharomyces cerevisiae
strain (WAT11) engineered for P450 expression. When recombinant yeast
microsomes were incubated with lauric acid (C12:0) for 15 min, one major
metabolite was formed.
We live in the age of biology—the human and many other organisms’
genomes have been sequenced and we are starting to understand the
function of the metabolic machinery responsible for life on our planet.
Thousands of new genes have been discovered, many of these coding for
enzymes of yet unknown function. Understanding the kinetic behavior
of an enzyme provides clues to its possible physiological role. From
a biotechnological point of view, knowledge of the catalytic properties
of an enzyme is required for the design of immobilized enzyme-based
Fatty acids are an important energy source, for they yield over twice as much energy as an equal mass of carbohydrate or protein. In humans, the primary dietary source of fatty acids is triacylglycerols. This lecture will describe the metabolism of fatty acids. The two main components of fatty acid metabolism are β oxidation and fatty acid synthesis. Upon completion of this lecture, you will understand that the fatty-acid breakdown reactions of β oxidation result in the formation of reduced cofactors and acetyl-CoA molecules, which can be further catabolized to release free energy.
The food we eat has a major effect on our physical health and psychological wellbeing.
An understanding of the way in which nutrients are metabolized, and hence of the
principles of biochemistry, is essential for an understanding of the scientific basis of
what we would call a prudent or healthy diet.
My aim in the following pages is to explain both the conclusions of the many
expert committees that have deliberated on the problems of nutritional requirements,
diet and health over the years and also the scientific basis on which these experts have
reached their conclusions.
PHOTOSYNTHESIS PROVIDES the organic building blocks that plants (and nearly all other life) depend on. Respiration, with its associated carbon metabolism, releases the energy stored in carbon compounds in a controlled manner for cellular use. At the same time it generates many carbon precursors for biosynthesis. In the first part of this chapter we will review respiration in its metabolic context, emphasizing the interconnections and the special features that are peculiar to plants.
The rhizome oil ofZingiber zerumbetSmith contains an exceptionally high
content of sesquiterpenoids with zerumbone, a predominating potential
multi-anticancer agent. Biosynthetic pathways of zerumbone have been
proposed, and two genesZSS1andCYP71BA1that encode the enzymes
catalyzing the first two steps have been cloned.
The efficient turnover of messenger RNA represents an important mecha-nism that allows the cell to control gene expression. Until recently, the
mechanism of mRNA decay was mainly attributed to exonucleases, com-prising enzymes that degrade RNAs from the ends of the molecules.
Một trong các cơ chế khu trú trao đổi chất phổ biến nhất là sự chia khoang (compartmentation) nghĩa là sự phân bố biệt hoá các enzyme và các chất trao đổi trong các cấu trúc tế bào tách biệt hoặc các bào quan có màng bao bọc. Chẳng hạn, sự oxy hoá acid béo gặp bên trong ti thể nhưng tổng hợp acid béo lại diễn ra trong tế bào chất.
Starch-binding domains are noncatalytic carbohydrate-binding modules
that mediate binding to granular starch. The starch-binding domains from
the carbohydrate-binding module family 45 (CBM45, http://www.cazy.org)
are found as N-terminal tandem repeats in a small number of enzymes,
primarily from photosynthesizing organisms.
The human pathogenic fungus Aspergillus fumigatus accumulates large
amounts of intracellular mannitol to enhance its resistance against defense
strategies of the infected host. To explore their currently unknown roles in
mannitol metabolism, we studied A. fumigatus mannitol-1-phosphate
5-dehydrogenase (AfM1PDH) and mannitol 2-dehydrogenase (AfM2DH)
Cytochrome P450 monooxygenases (P450s) are a diverse collection of
enzymes acting on various endogenous and xenobiotic molecules. Most of
them catalyse hydroxylation reactions and one group of possible substrates
are fatty acids and their related structures. In this minireview, the signifi-cance of P450s in microbial fatty acid conversion is described.
Several bacteria, including Azotobacter vinelandii, possess an alternative
pathway ofl-rhamnose metabolism, which is different from the known
bacterial pathway. In a previous article, a gene cluster related to this path-way was identified, consisting of the genes encoding the four metabolic
enzymes l-rhamnose-1-dehydrogenase (LRA1), l-rhamnono-c-lactonase
Chapter 10 introduction to metabolism. After studying this chapter you will be able to: Define catabolism and anabolism and know general properties regarding each, know the difference between a heterotroph and a autotroph, know the general spatial organization of pathways (multienzyme complexes, individual soluble proteins, membrane associated),...
Chapter 17 (part 1) - Amino acid metabolism: Nitrogen assimilation and amino acid biosynthesis. After completing this chapter, students will be able to: Know how different forms of nitrogen get to assimilatable form, know what types of organisms have this enzyme, know the reactions catalyzed by the enzymes below. Know structure of substrates and products, know co-enzymes and co-substrates involved,...
The kynurenine pathway of tryptophan catabolism plays an important role
in several biological systems affected by aging. We quantified tryptophan and
its metabolites kynurenine (KYN), kynurenine acid (KYNA), picolinic acid
(PIC) and quinolinic acid (QUIN), and activity of the kynurenine pathway
enzymes indoleamine 2,3-dioxygenase