The Dnmt3a DNA methyltransferase is responsible for establishing DNA
methylation patterns during mammalian development. We show here that
the mouse Dnmt3a DNA methyltransferase is able to transfer the methyl
group fromS-adenosyl-L-methionine (AdoMet) to a cysteine residue in its
catalytic center. This reaction is irreversible and relatively slow.
Protein arginine methyltransferase (PRMT)1 is the predominant type I
methyltransferase in mammals. In the present study, we used zebrafish
(Danio rerio) as the model system to elucidate PRMT1 expression and
function during embryogenesis.
Genomic sequences ofHelicobacter pyloristrains 26695, J99, HPAGI and
G27 have revealed an abundance of restriction and modification genes.
hp0050, which encodes an N
adenine DNA methyltransferase, was cloned,
overexpressed and purified to near homogeneity.
The fourth lysine of histone H3 is post-translationally modified by a methyl
group via the action of histone methyltransferase, and such a covalent
modification is associated with transcriptionally active and⁄or repressed
chromatin states. Thus, histone H3 lysine 4 methylation has a crucial role
in maintaining normal cellular functions.
DNA methylation affects important developmental processes in both plants
and animals. The process of methylation of cytosines at C-5 is catalysed by
DNA methyltransferases (MTases), which are highly conserved, both struc-turally and functionally, in eukaryotes. In this study, we identified and
characterized cytosine DNA MTase genes that are activated with the onset
of reproductive development in rice.
Bile salts are potent detergents and can disrupt cellular membranes, which
causes cholestasis and hepatocellular injury. However, the mechanism for
the resistance of the canalicular membrane against bile salts is not clear.
Phosphatidylethanolamine (PE) is converted to phosphatidylcholine (PC)
in the liver by phosphatidylethanolamine N-methyltransferase (PEMT).
The protein arginine methyltransferase (PRMT) family of enzymes cata-lyzes the transfer of methyl groups from S-adenosylmethionine to the gua-nidino nitrogen atom of peptidylarginine to form monomethylarginine or
dimethylarginine. We created several less polar analogs of the specific
PRMT inhibitor arginine methylation inhibitor-1, and one such compound
was found to have improved PRMT inhibitory activity over the parent
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Catechol-O-methyltransferase gene haplotypes in Mexican and Spanish patients with fibromyalgia...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: The SET -domain protein superfamily: protein lysine methyltransferases...
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Continuous and low-energy 125I seed irradiation changes DNA methyltransferases expression patterns and inhibits pancreatic cancer tumor growth
The Bowman–Birk protease inhibitors have recently attracted attention for
their potential as cancer preventive and suppressing agents. They contain
two canonical binding loops, both consisting of nine highly conserved resi-dues capable of inhibiting corresponding serine proteases.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: RFX1 regulates CD70 and CD11a expression in lupus T cells by recruiting the histone methyltransferase SUV39H1...
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Genetic variation of g-tocopherol methyltransferase gene contributes to elevated a-tocopherol content in soybean seeds
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize (Zea mays L.)
Wheat flavoneO-methyltransferase catalyzes three sequential methylations
of the flavone tricetin. Like other flavonoidO-methyltransferases, the pro-tein is a homodimer. We demonstrate, using analytical ultracentrifugation,
that perchlorate dissociates the dimer into monomers. The resulting mono-mers retain all their catalytic capacity, including the ability to catalyze the
three successive methylations.
Plants belonging to the Apiaceae or Rutaceae accumulate
methoxylated psoralens, such as bergapten or xanthotoxin,
as the final products of their furanocoumarin biosynthesis,
and the rate of accumulationdepends on environmental and
other cues. DistinctO-methyltransferase activities had been
reported to methylate bergaptol to bergapten and xantho-toxol to xanthotoxin, from induced cell cultures of Ruta
graveolens,Petroselinum crispumandAmmi majus. Bergap-tol 5-O-methyltransferase (BMT) cDNA was cloned from
dark-grownAmmi majusL. cells treatedwith a crude fungal
EcoRII DNA methyltransferase (M.EcoRII) recognizes
the 5¢…CC*T/AGG…3¢ DNA sequence and catalyzes the
transfer of the methyl group fromS-adenosyl-L-methionine
to the C5 position of the inner cytosine residue (C*). Here,
we study themechanismof inhibitionofM.EcoRII byDNA
containing 2-pyrimidinone, a cytosine analogue lacking an
group at the C4 position of the pyrimidine ring. Also,
DNA containing 2-pyrimidinone was used for probing
contacts ofM.EcoRII with functional groups of pyrimidine
bases of the recognition sequence....
Tocopherols are essential micronutrients in human and
animal nutrition due to their function as lipophilic anti-oxidants. Theyare exclusively synthesizedbyphotosynthetic
organisms including higher plants. Despite the attributed
beneficial health effects and many industrial applications,
research on the tocopherol biosynthetic pathway and its
regulation inplants is still limited.
Methanol:coenzyme M methyltransferase from methanogenic archaea is a cobalamin-dependent enzyme composed of three diﬀerent subunits: MtaA, MtaB and MtaC. MtaA is a zinc protein that catalyzes the methylation of coenzyme M (HS-CoM) with methylcob(III)alamin. We report zinc XAFS (X-ray absorption ﬁne structure) results indicating that, in the absence of coenzyme M, zinc is probably coordinated by a single sulfur ligand and three oxygen or nitrogen ligands. In the presence of coenzyme M, one (N/O)-ligand was replaced by sulfur, most likely due to ligation of the thiol group of coenzyme M....