Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Modulation of microtubule assembly by the HIV-1 Tat protein is strongly dependent on zinc binding to Tat...
RasGRF is a family of guanine nucleotide exchange factors with dual spe-cificity for both Ras and Rac GTPases. In this study, using mouse brain
extracts, we show that both RasGRF1 and RasGRF2 interact with micro-tubules in an in vitro microtubule assembly system and this binding is very
tight. To characterize this association, recombinant purified proteins con-taining different regions of RasGRF1 were tested for their ability to bind
microtubules preassembled from pure tubulin.
Sanguinarine has been shown to inhibit proliferation of several types of
human cancer cell including multidrug-resistant cells, whereas it has min-imal cytotoxicity against normal cells such as neutrophils and keratino-cytes. By analyzing the antiproliferative activity of sanguinarine in relation
to its effects on mitosis and microtubule assembly, we found that it inhibits
cancer cell proliferation by a novel mechanism.
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells
The kinase noncatalytic C-lobe domain (KIND) is a putative protein–protein
interaction module. Four KIND-containing proteins, Spir-2 (actin-nuclear
factor), PTPN13 (protein tyrosine phosphatase), FRMPD2 (scaffold protein)
and very-KIND (v-KIND) (brain-specific Ras guanine nucleotide exchange
factor), have been identified to date.
In this study, curcumin, a potential anticancer agent, was found to dampen
the dynamic instability of individual microtubules in living MCF-7 cells. It
strongly reduced the rate and extent of shortening states, and modestly
reduced the rate and extent of growing states. In addition, curcumin
decreased the fraction of time microtubules spent in the growing state and
strongly increased the time microtubules spent in the pause state.
Curcumin, a component of turmeric, has potent antitumor activity against
several tumor types. However, its molecular target and mechanism of anti-proliferative activity are not clear. Here, we identified curcumin as a novel
antimicrotubule agent. We have examined the effects of curcumin on cellu-lar microtubules and on reconstituted microtubules in vitro.
Heterotrimeric G proteins participate in signal transduction by transferring
signals from cell surface receptors to intracellular effector molecules.
G proteins also interact with microtubules and participate in microtubule-dependent centrosome⁄chromosome movement during cell division, as well
as neuronal differentiation.
We found that benomyl, a benzimidazole fungicide, strongly suppressed
the reassembly of cold-depolymerized spindle microtubules in HeLa cells.
Benomyl perturbed microtubule-kinetochore attachment and chromosome
alignment at the metaphase plate. Benomyl also significantly decreased
the distance between the sister kinetochore pairs in metaphase cells and
increased the level of the checkpoint protein BubR1 at the kinetochore
region, indicating that benomyl caused loss of tension across the kineto-chores....
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Specific microtubule-depolymerizing agents augment efficacy of dendritic cell-based cancer vaccines
Taxanes and other microtubule-stabilizing agents comprise an important
class of anticancer drugs. It is well known that taxanes act by binding to
b-tubulin on the lumenal side of microtubules. However, experimental evi-dence obtained in recent years led to the hypothesis of an external site on
the microtubule wall to which taxanes and other microtubule-stabilizing
agents could bind before being internalized to their lumenal site.
In many laboratories, the requirement of microtubule-associated proteins
(MAPs) and the stabilization of microtubules for the elongation of neurites
has been intensively investigated, with controversial results being obtained.
We have observed that the neurite microtubules of Cath.a-differentiated
(CAD) cells, a mouse brain derived cell, are highly dynamic structures, and
so we analyzed several aspects of the cytoskeleton to investigate the molecu-lar causes of this phenomenon.
Rotenone, a widely used insecticide, has been shown to inhibit mammalian
cell proliferation and to depolymerize cellular microtubules. In the present
study, the effects of rotenone on the assembly of microtubules in relation
to its ability to inhibit cell proliferation and mitosis were analyzed.
Phosphorylation of tau protein modulates both its physiological role and
its aggregation into paired helical fragments, as observed in Alzheimer’s
diseased neurons. It is of fundamental importance to study paired helical
fragment formation and its modulation by phosphorylation. This study
focused on the fourth microtubule-binding repeat of tau, encompassing an
abnormal phosphorylation site, Ser356.