The antibiotic heliquinomycin, which inhibits cellular DNA replication at a
half-maximal inhibitory concentration (IC50) of 1.4–4lm, was found to inhi-bit the DNA helicase activity of the human minichromosome maintenance
(MCM) 4/6/7 complex at an IC50value of 2.4lm. In contrast, 14 lmheliqui-nomycin did not inhibit significantly either the DNA helicase activity of the
SV40 T antigen and Werner protein or the oligonucleotide displacement
activity of human replication protein A.
DNA replication, the process of copying one double stranded DNA molecule to produce two identical copies, is at the heart of cell proliferation. This book highlights new insights into the replication process in eukaryotes, from the assembly of pre-replication complex and features of DNA replication origins, through polymerization mechanisms, to propagation of epigenetic states. It also covers cell cycle control of replication initiation and includes the latest on mechanisms of replication in prokaryotes. The association between genome replication and transcription is also addressed.
Minichromosome maintenance proteins (Mcm) 2–7 play
essential roles in eukaryotic DNA replication. Several
reports have indicated the usefulness of Mcm proteins as
markers of cancer cells in histopathological diagnosis.
However, their mode of expression and pathophysiological
significance in cancer cells remain to be clarified. We com-paredthe level of expressionofMcmproteins amonghuman
HeLa uterine cervical carcinoma cells, SV40-transformed
human fibroblast GM00637 cells andnormal human fibro-blastWI-38 cells....
Minichromosomemaintenance (MCM) proteins are part of
the replication licensing factor (RLF-M), which limits the
initiationofDNAreplication to once per cell cycle.We have
previously reported that higher order complexes of mam-malian pol II and general pol II transcription factors,
referred to as pol II holoenzyme, also contain MCM pro-teins. In the present study we have analyzed in detail the
interaction between MCM2 and pol II holoenzyme. N- and
C- terminal deletions were introduced into epitope-tagged
MCM2 and the truncated proteins were transiently
expressed in 293 cells. ...