Cyclins are the essential regulatory subunits of cyclin-dependent protein
kinases. They accumulate and disappear periodically at specific phases of
the cell cycle. Here we investigated whether variations in cyclin mRNA
levels in exponentially growing cells can be attributed to changes in mRNA
stability. Mouse EL4 lymphoma cells and 3T3 fibroblasts were synchron-ized by elutriation or cell sorting.
Proteins are the driving force for all cellular processes. They regulate several cellular
events through binding to different partners in the cell. They are capable of binding to
other proteins, peptides, DNA, and also RNA. These interactions are essential in the
regulation of cell fates and could be important in drugs development. For example
RNA interacting proteins regulate gene expression through the binding to different
mRNAs. These mRNAs could be involved in important cellular processes such as cell
survival or apoptosis.
Vascular endothelial growth factor (VEGF) is a key regu-lator of angiogenesis and post-transcriptional regulation
plays a major role in VEGF expression. Both the 5¢-and
3¢-UTR are required for VEGF post-transcriptional regu-lation but factors binding to functional sequences within
the 5¢-UTR have not been fully characterized. We report
here the identification of complexes, binding to theVEGF
mRNA 5¢-and3¢-UTR, that contain cold shock domain
(CSD) and polypyrimidine tract binding (PTB) RNA
binding proteins. ...
Poly(A)-specific ribonuclease (PARN) specifically catalyzes the degradation
of the poly(A) tails of single-stranded mRNAs in a highly processive mode.
PARN participates in diverse and important intracellular processes by act-ing as a regulator of mRNA stability and translational efficiency. In this
article, the equilibrium unfolding of PARN was studied using both guani-dine hydrochloride and urea as chemical denaturants.
The expression of the major protein kinase C substrate
MARCKS (myristoylatedalanine-richCkinase substrate) is
controlled by the stability of its mRNA. While the
MARCKS mRNA is long living in quiescent fibroblasts
(t1/2¼14 h), its half-life time is drastically reduced
(t1/2¼2 h) in cells treated with phorbol esters to activate
protein kinase C (PKC) or treatedwith growth factors. In a
first step to study the underlying mechanism we identified
both acis-element on the MARCKS mRNA and the cor-responding trans-acting factors. ...
mRNA stabilization plays an important role in the changes
in protein expression initiated by inducers of inflammation
or direct cell stress such as UV light. This study provides
evidence that stabilization in response to UV light differs
from that induced by proinflammatory stimuli such as
bacterial lipopolysaccharide or interleukin (IL)-1. Firstly,
UV-induced stabilization is independent of the p38 MAP
kinase pathway, which has previously been shown to medi-ate stabilization induced by IL-1 or lipopolysaccharide....
Induction of p21CIP1/WAF1 expression by human T-lymphotropic virus type 1 Tax requires transcriptional activation and mRNA stabilization
Ling Zhang, Huijun Zhi, Meihong Liu, Yu-Liang Kuo and Chou-Zen Giam*
Address: Department of Microbiology and Immunology, Uniformed Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA Email: Ling Zhang - firstname.lastname@example.org; Huijun Zhi - email@example.com; Meihong Liu - firstname.lastname@example.org; YuLiang Kuo - email@example.com; Chou-Zen Giam* - cgiam@usuhs.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Evidence for selection on synonymous mutations affecting stability of mRNA secondary structure in mammals...
Poly(A) binding protein (PABP) binds mRNApoly(A) tails
and affects mRNA stability and translation. We show here
that there is little free PABP in NIH3T3 cells, with the vast
majority complexed with RNA. We found that PABP
in NIH3T3 cytoplasmic lysates and recombinant human
PABP can bind toAU-richRNA with high affinity. Human
PABP bound an AU-rich RNA withKdin the nMrange,
which was only sixfold weaker than the affinity for oligo(A)
Connective tissue growth factor (CTGF/CCN2) is an
immediate early gene-encoded polypeptide modulating cell
growth and collagen synthesis. The importance of CTGF/
CCN2 function is highlighted by its disregulation in fibrotic
disorders. In this study, we investigated the regulation and
signaling pathways that are required for various stimuli of
intracellular signaling events to induce the expression of the
endogenous CTGF/CCN2gene in smooth muscle cells.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: A catalog of stability-associated sequence elements in 3' UTRs of yeast mRNAs...
Polypyrimidine tract-binding protein (PTB) is a widely expressed RNA-binding protein with multiple roles in RNA processing, including the splic-ing of alternative exons, mRNA stability, mRNA localization, and internal
ribosome entry site-dependent translation. Although it has been reported
that increased expression of PTB is correlated with cancer cell growth, the
role of PTB in mammalian development is still unclear.
important regulators of biological processes in animals
and plants. MiRNAs regulate gene expression at
the posttranscriptional level by binding to mRNAs and
either inhibit translation or modify the stability of the
mRNA. Due to the important biological role of miRNAs
it is of great interest to study their expression level in
the cells. Furthermore, miRNAs have been associated
with cancer and other diseases  and miRNA expression
can help in the diagnosis and prognostic of human
We examined the effect of suboptimal concentrations of
cyclin-dependent kinase inhibitors, which do not interfere
with cell proliferation, on retinoblastoma expression in
hamster (Chinese hamster ovaryK1) and human (K562 and
HeLa) cells. To achieve this, we used the chemical inhibitors
roscovitine and olomoucine (which inhibit CDK2 prefer-entially),UCN-01 (which also inhibits CDK4/6) andp21 (as
an intrinsic inhibitor). All chemical inhibitors and over-expression of p21 strongly induced retinoblastoma protein
PUF proteins regulate both stability and translation through sequence-spe-cific binding to the 3¢ UTR of target mRNA transcripts. Binding is medi-ated by a conserved PUF domain, which contains eight repeats of
approximately 36 amino acids each. Found in all eukaryotes, they have
been related to several developmental processes.