Activation of the c-JUN N-terminal kinase (JNK) pathway is implicated in
a number of important physiological processes, from embryonic morpho-genesis to cell survival and apoptosis. JNK stimulatory phosphatase 1
(JSP1) is a member of the dual-specificity phosphatase subfamily of protein
In order to determine the amino-terminal sequence
requirements for protein N-myristoylation, site-directed
mutagenesis of the N-terminal region was performed using
tumor necrosis factor (TNF) mutants as model substrate
proteins. Subsequently, the susceptibility of thesemutants to
protein N-myristoylation was evaluated by metabolic labe-ling in anin vitrotranslation systemusing rabbit reticulocyte
lysate. A TNF mutant having the sequence MGAAAAA
AAA at its N-terminus was used as the starting sequence
to identify elements critical for protein N-myristoylation....
Protein N-myristoylation plays key roles in various cellular functions in
eukaryotic organisms. To clarify the relationship between the efficiency of
protein N-myristoylation and the amino acid sequence of the substrate in
plants, we have applied a wheat germ cell-free translation system with high
protein productivity to examine the N-myristoylation of various wild-type
and mutant forms ofArabidopsis thalianaproteins.
The N-terminally myristoylated, 19-amino acid peptide, corresponding to
the amino terminus of the neuronal protein NAP-22 (NAP-22 peptide) is a
naturally occurring peptide that had been shown by fluorescence to cause
the sequestering of a Bodipy-labeled PtdIns(4,5)P2 in a cholesterol-depend-ent manner. The present work, using differential scanning calorimetry
(DSC), extends the observation that formation of a PtdIns(4,5)P2-rich
domain is cholesterol dependent and shows that it also leads to the forma-tion of a cholesterol-depleted domain. ...
A hydrophilic cation-binding protein, PCaP1, was found to be stably
bound to the plasma membrane inArabidopsis thaliana. PCaP1 was quanti-fied to account for 0.03–0.08% of the crude membrane fractions from roots
HR12 is anovel farnesyltransferase inhibitor (FTI).Wehave
shown previously that HR12 induces phenotypic reversion
-transformed Rat1 (Rat1/ras) fibroblasts. This
reversion was characterized by formation of cell–cell con-tacts, focal adhesions and stress fibers. Here we show that
HR12 inhibits anchorage independent and dependent
growth of Rat1/ras cells. HR12 also suppresses motility and
proliferation of Rat1/ras cells, in a wound healing assay.
Rat1 fibroblasts transformed with myristoylated H-ras
(Rat1/myr-ras) were resistant to HR12....
In rod phototransduction, cyclic GMP synthesis by mem-brane bound guanylate cyclase ROS-GC1 is under Ca
-dependent negative feedback control mediated by guanylate
cyclase-activating proteins, GCAP-1 and GCAP-2. The
cellular concentration of GCAP-1 and GCAP-2 approxi-mately sums to the cellular concentration of a functional
ROS-GC1 dimer. Both GCAPs increase the catalytic effi-ciency (kcat
/Km) of ROS-GC1. However, the presence of a
myristoyl group in GCAP-1 has a strong impact on the
regulation of ROS-GC1, this is in contrast to GCAP-2....