Plasma fraction

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  • Anemia occurs in ~80% of myeloma patients. It is usually normocytic and normochromic and related both to the replacement of normal marrow by expanding tumor cells and to the inhibition of hematopoiesis by factors made by the tumor. In addition, mild hemolysis may contribute to the anemia. A larger than expected fraction of patients may have megaloblastic anemia due to either folate or vitamin B12 deficiency. Granulocytopenia and thrombocytopenia are very rare.

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  • The purpose of this monograph is to formulate a quantitative and self-consistent theoretical approach to wave–particle interactions occurring in space plasmas, and present a logical development of the subject. In the Earth’s magnetosphere, Nature has given us a plasma laboratory that is accessible to observations made by radio, magnetic and electric instruments on the ground, and a great variety of instruments aboard rockets and Earth-orbiting satellites. Spacecraft are making similar observations in the more distant solar system.

    pdf366p tom_123 14-11-2012 13 3   Download

  • The first edition of Blood Transfusion in Clinical Medicine was published in 1951, at a time when the subject was, if not in its infancy, certainly in its very early childhood. Transfusions were given for the treatment of acute blood loss or for the relief of chronic anaemia. Platelet and leucocyte transfusions were not attempted and plasma fractions were not available.

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  • A hydrophilic cation-binding protein, PCaP1, was found to be stably bound to the plasma membrane inArabidopsis thaliana. PCaP1 was quanti-fied to account for 0.03–0.08% of the crude membrane fractions from roots and shoots.

    pdf16p media19 06-03-2013 13 2   Download

  • We havemade a comprehensive and quantitative analysis of the lipid composition of caveolae from primary rat fat cells and compared the composition of plasma membrane inside and outside caveolae. We isolated caveolae from purified plasmamembranesusingultrasonication incarbonatebuffer to disrupt the membrane, or extraction with nonionic detergent, followed by density gradient ultracentrifugation. The carbonate-isolated caveolae fraction was further immunopurified using caveolin antibodies.

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  • Leukocytes and other cells show an enhanced intensity of mobile lipid in their 1 H NMR spectra under a variety of conditions.Such conditions include stimulation, which has recently been shown to involve detergent-resistant, plasma membranedomains (DRMs)oftencalled lipidrafts.As there ismuchspeculationsurrounding theoriginof cellularNMR-visible lipid, we analysed subcellular fractions, including DRMs, by NMR spectroscopy.

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  • Nongenomic action of an insect steroid hormone, 20-hy-droxyecdysone (20E), has been implicated in several 20E-dependent events including the programmed cell death of Bombyxanterior silk glands (ASGs), but no information is available for themodeof theaction.Weprovide evidence for a putative membrane receptor located in the plasma mem-brane of the ASGs. Membrane fractions prepared from the ASGs exhibit high binding activity to [ 3 H]ponasterone A (PonA).

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  • Bilitranslocase is a carrier protein localized at the basolateral domain of the hepatocyte plasma membrane. It transports various organic anions, including bromosulfophthalein and anthocyanins. Functional studies in subcellular fractions enriched in plasma membrane revealed a high-affinity binding site for bilirubin, associated with bilitranslocase.

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  • Antioxidant protein 2 (AOP2) is a member of a family of thiol-specific antioxidants, recently renamedperoxiredoxins, that evolvedas part of anelaborate systemtocounteract and control detrimental effects of oxygen radicals. AOP2 is found in endothelial cells, erythrocytes, monocytes, T andB cells, but not in granulocytes. AOP2 was found solely in the cytoplasm and was not associated with the nuclear or membrane fractions; neither was it detectable in plasma.

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  • The protein-modifying agent arsenite stimulates glucose uptake in 3T3-L1 adipocytes. In the current study we have analysed the signalling pathways that contribute to this response. By subcellular fractionation we observed that arsenite, like insulin, induces translocation of the GLUT1 and GLUT4 glucose transporters from the low-density membrane fraction to the plasma membrane. Arsenite did not activate early steps of the insulin receptor (IR)-signalling pathway and the response was insensitive to inhibition of phosphatidylinositol-3¢-kinase (PI-3¢)kinaseby wortman-nin. ...

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