Anemia occurs in ~80% of myeloma patients. It is usually normocytic and normochromic and related both to the replacement of normal marrow by expanding tumor cells and to the inhibition of hematopoiesis by factors made by the tumor. In addition, mild hemolysis may contribute to the anemia. A larger than expected fraction of patients may have megaloblastic anemia due to either folate or vitamin B12 deficiency. Granulocytopenia and thrombocytopenia are very rare.
The purpose of this monograph is to formulate a quantitative and self-consistent theoretical
approach to wave–particle interactions occurring in space plasmas, and present
a logical development of the subject. In the Earth’s magnetosphere, Nature has given
us a plasma laboratory that is accessible to observations made by radio, magnetic and
electric instruments on the ground, and a great variety of instruments aboard rockets
and Earth-orbiting satellites. Spacecraft are making similar observations in the more
distant solar system.
The first edition of Blood Transfusion in Clinical
Medicine was published in 1951, at a time when the
subject was, if not in its infancy, certainly in its very
early childhood. Transfusions were given for the treatment
of acute blood loss or for the relief of chronic
anaemia. Platelet and leucocyte transfusions were not
attempted and plasma fractions were not available.
A hydrophilic cation-binding protein, PCaP1, was found to be stably
bound to the plasma membrane inArabidopsis thaliana. PCaP1 was quanti-fied to account for 0.03–0.08% of the crude membrane fractions from roots
We havemade a comprehensive and quantitative analysis of
the lipid composition of caveolae from primary rat fat cells
and compared the composition of plasma membrane inside
and outside caveolae. We isolated caveolae from purified
to disrupt the membrane, or extraction with nonionic
detergent, followed by density gradient ultracentrifugation.
The carbonate-isolated caveolae fraction was further
immunopurified using caveolin antibodies.
Leukocytes and other cells show an enhanced intensity of
mobile lipid in their
H NMR spectra under a variety of
conditions.Such conditions include stimulation, which has
recently been shown to involve detergent-resistant, plasma
membranedomains (DRMs)oftencalled lipidrafts.As there
ismuchspeculationsurrounding theoriginof cellularNMR-visible lipid, we analysed subcellular fractions, including
DRMs, by NMR spectroscopy.
Nongenomic action of an insect steroid hormone, 20-hy-droxyecdysone (20E), has been implicated in several 20E-dependent events including the programmed cell death of
Bombyxanterior silk glands (ASGs), but no information is
available for themodeof theaction.Weprovide evidence for
a putative membrane receptor located in the plasma mem-brane of the ASGs. Membrane fractions prepared from the
ASGs exhibit high binding activity to [
Bilitranslocase is a carrier protein localized at the basolateral domain of
the hepatocyte plasma membrane. It transports various organic anions,
including bromosulfophthalein and anthocyanins. Functional studies in
subcellular fractions enriched in plasma membrane revealed a high-affinity
binding site for bilirubin, associated with bilitranslocase.
Antioxidant protein 2 (AOP2) is a member of a family of
thiol-specific antioxidants, recently renamedperoxiredoxins,
that evolvedas part of anelaborate systemtocounteract and
control detrimental effects of oxygen radicals. AOP2 is
found in endothelial cells, erythrocytes, monocytes, T andB
cells, but not in granulocytes. AOP2 was found solely in the
cytoplasm and was not associated with the nuclear or
membrane fractions; neither was it detectable in plasma.
The protein-modifying agent arsenite stimulates glucose
uptake in 3T3-L1 adipocytes. In the current study we have
analysed the signalling pathways that contribute to this
response. By subcellular fractionation we observed that
arsenite, like insulin, induces translocation of the GLUT1
and GLUT4 glucose transporters from the low-density
membrane fraction to the plasma membrane. Arsenite did
not activate early steps of the insulin receptor (IR)-signalling
pathway and the response was insensitive to inhibition of
phosphatidylinositol-3¢-kinase (PI-3¢)kinaseby wortman-nin. ...