Biomedical research has entered a new era of characterizing a disease or a protein on a global scale. In the post-genomic era, Proteomics now plays an increasingly important role in dissecting molecular functions of proteins and discovering biomarkers in human diseases. Mass spectrometry, two-dimensional gel electrophoresis, and high-density antibody and protein arrays are some of the most commonly used methods in the Proteomics field.
Enzymes are proteins that catalyze chemical reactions. A protein is simply a polypeptide
composed of amino acids linked by a peptide bond, and the term generally, but
not always, refers to the folded conformation. To understand how an enzyme functions,
including its binding and functional properties, it is necessary to know the
properties of the amino acids and how the amino acids are linked together, including
the torsion angles of the bonds and the space occupied, and the interactions of the
atoms leading to the final conformations of the folded protein.