The current volume entitled Protein Purification is designed to facilitate rapid access to valuable information about various methodologies. It aims as well to provide an overview of state-of-art techniques for the purification, analysis and quantification of proteins in complex samples using different enrichment strategies.Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest....
In this chapter 3 (part 2), you will: Know why it is important to purify a protein, know what organelles are in the pellet/supernatant during the differential centrifugation of a cell extract, understand how gel permeation chromatography works and what you can learn from this analysis, understand how ion exchange chromatography works,...
The field of biochemistry is diverse and forms parts of diverse
fields including cell biology, molecular biology and medical sciences.
Biochemistry is the study of the molecules of life like
proteins, lipids, carbohydrates and nucleic acids. Studying the
structure, properties and reactions of these important molecules
would help in better understanding life as a whole. The practical
aspect along with the theoretical background would help
in better understanding these mechanisms. This book tries to
address and compile some of the routinely used protocols in biochemistry
for easy access.
was perhaps a mark of how successful the second edition of Instant Notes in Biochemistry was that we
recall seeing a final year student avidly reading it even as he waited to have his viva with the External
Examiner. Although we would strongly recommend to any student not to leave revision to such a very
late stage, this experience alone proved the value of a concise book that focused on essential biochemical
information in an easily accessible format!
Let us be clear.