Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C
In this review, we first describe the mechanisms by which the epidermal
growth factor receptor generates a Ca
signal and, subsequently, we com-pile the available experimental evidence regarding the role that the
⁄calmodulin complex, formed after the rise in cytosolic free Ca
concentration, exerts on the receptor.
To help you further specialized material Chemistry serving the needs of learning and research, invite you to refer to the contents of the document "Accleration of fish sauce fermentation using proteolytic enzymes". Hope content useful document for the learning needs and research.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: A novel approach to measure the contribution of matrix metalloproteinase in the overall net proteolytic activity present in synovial fluids of patients with arthritis...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: The mutation T477A in HIV-1 reverse transcriptase (RT) restores normal proteolytic processing of RT in virus with Gag-Pol mutated in the p51-RNH cleavage site...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: A pathway sensor for genome-wide screens of intracellular proteolytic cleavage...
Dentatorubral-pallidoluysian atrophy is caused by polyglutamine (polyQ)
expansion in atrophin-1 (ATN1). Recent studies have shown that nuclear
accumulation of ATN1 and cleaved fragments with expanded polyQ is the
pathological process underlying neurodegeneration in dentatorubral-pallid-oluysian atrophy.
Duodenase is a 29-kDa serine endopeptidase that displays selective trypsin- and chymotrypsin-like substrate speciﬁcity. This enzyme has been localized to epitheliocytes of Brunner’s glands, and as described here, to mast cells within the intestinal mucosa and lungworm-infected lung, implying an important additional role in inﬂammation and tissue remodelling. In primary cultures of pulmonary artery ﬁbroblasts, duodenase induced a concentration-dependent increase in [3H]thymidine incorporation with a maximal eﬀect observed at 30 nM.
In avian species, an egg envelope homologous to the mammalian zona pellucida is called the perivitelline membrane. We have previously reported that one of its components, a glycoprotein homologous to mammalian ZPC, is synthesized in the granulosa cells of the quail ovary. In the present study, we investigated the proteolytic cleavage of the newly synthesized ZPC and the secretion of ZPC from the granulosa cells. Western blot analysis of the cell lysates demonstrated that the 43-kDa protein is the precursor of mature ZPC (proZPC), and is converted to the 35-kDa protein before secretion. ...
Interaction of cytokines with their cognate receptors leads
to the activation of latent transcription factors, the signal
transducer and activator of transcription (STAT) proteins.
Numerous studies have identified the critical roles played
by STAT proteins in regulating cell proliferation, differ-entiation and survival. Consequently, the activity of STAT
proteins is negatively regulated by a variety of different
mechanisms, which include alternative splicing, covalent
modifications, protein–protein interactions with negative
regulatory proteins and proteolytic processing by pro-teases....
ATP-dependent Lon proteases belong to the superfamily of
proteins. Until recently, the identity of the residues
involved in their proteolytic active sites was not elucidated.
However, the putative catalytic Ser–Lys dyad was recently
suggested through sequence comparison of more than 100
Lon proteases from various sources. The presence of the
catalytic dyadwas experimentally confirmedby site-directed
mutagenesis of theEscherichia coliLon protease and by
determination of the crystal structure of its proteolytic
Tiêu hoá ở ruột Quá trình tiêu hoá các chất dinh dưỡng đều xảy ra ở ruột non của chim. Nguồn các men tiêu hoá quan trọng nhất là từ dịch dạ dày, cùng với mật đi vào manh tràng, chất tiết của các tuyến ruột có ý nghĩa kém hơn. Dịch ruột chim là một chất lỏng đục, có phản ứng kiềm yếu (pH - 7,42) với tỷ trọng 1,0076. Trong thành phần dịch ruột có các men proteolytic, aminolytic và lypolytic và ả men c enterokinaza. Dịch tuỵ là một chất lỏng không màu, hơi mặn, có...
Protease còn được gọi là các proteolytic enzym, là các enzym có khả năng thủy phân các liên kết peptid của chuỗi peptid, protein thành các đoạn peptid ngắn hơn và các acid amin. Các protease có thể hoạt động như các yếu tố phát triển của cả tế bào ác tính và tế bào bình thường, trong sự phân chia tế bào, sinh tổng hợp ADN....
DPP IV has been attributed a large array of functions, some of which are mediated by
its exopeptidase activity. Although it only removes two amino acid residues at the N-terminus
of the peptide, this cleavage can inactivate or modify the activity of regulatory peptides, peptide
hormones, chemokines and neuropeptides. Several excellent DPP IV substrates with high
specificity constants were identified by the in vitro kinetic study of the truncation of bioactive
peptides by DPP IV. In vivo studies e.g.
The complexes formed by partially folded human and bovine
a-lactalbumin with oleic acid (OA) have been reported to display selective
apoptotic activity against tumor cells. These complexes were named human
(HAMLET) or bovine (BAMLET) alpha-lactalbumin made lethal to
Latex of allVasconcelleaspecies analyzed to date exhibits higher proteolytic
amidase activities, generally attributed to cysteine proteinases, than the
latex of Carica papaya. In the present study, we show that this higher activ-ity is correlated with a higher concentration of enzymes in the latex of Vas-concellea fruits, but in addition also results from the presence of other
cysteine proteinases or isoforms.
Cyclins form complexes with cyclin-dependent kinases. By controlling
activity of the enzymes, cyclins regulate progression through the cell cycle.
A- and B-type cyclins were discovered due to their distinct appearance in
S and G2
phases and their rapid proteolytic destruction during mitosis.
Transition from G2 to mitosis is basically controlled by B-type cyclins.
The neutral N-glycan structures of Arion lusitanicus (gas-tropod) skin, viscera and egg glycoproteins were examined
after proteolytic digestion, release of the glycans from the
peptides, fluorescent labelling with 2-aminopyridine and
fractionation by charge, size and hydrophobicity to obtain
pure glycan structures. The positions and linkages of the
sugars in the glycan were analysed by two dimensional
HPLC (size and hydrophobicity) and MALDI-TOF mass
spectrometry before and after digestion with specific
Murine pregnancy-associated plasma protein-A (PAPP-A) cDNA encoding a 1545 amino-acid protein has been cloned. We have also identiﬁed and cloned cDNA that encodes a novel variant of PAPP-A, PAPP-Ai, carrying a 29-residue highly basic insert. The point of insertion corresponds to a junction between two exons in the human PAPP-A gene. The human intron ﬂanked by these exons does not encode a homologous corresponding insert, which is unique to the mouse.
It is essential to subculture the cells once cultured cells reach confluence. For this, trypsin is frequently applied to dissociate adhesive cells from the substratum. However, due to the proteolytic activity of trypsin, cell surface proteins are often cleaved, which leads to dysregulation of the cell functions. Methods: In this study, a triplicate 2D-DIGE strategy has been performed to monitor trypsin-induced proteome alterations. The differentially expressed spots were identified by MALDI-TOF MS and validated by...