Tiểu luận Kỹ thuật di truyền với đề tài "Kỹ thuật Rapd (Random amplified polymorphic DNA)" trình bày nội dung sau: giới thiệu, nguyên lý, các ưu nhược điểm của kỹ thuật Rapd, những khác biệt của Rapd với PCR, ứng dụng, những cải tiến của kỹ thuật Rapd.
The development and rapid implementation of molecular genotyping methods have revolutionized
the possibility for differentiation and classification of microorganisms at the
subspecies level. Investigation of the species diversity is required to determine molecular
relatedness of isolates for epidemiological studies. Methods for molecular epidemiology
of microorganisms must be highly reproducible and provide effective discrimination of
epidemiologically unrelated strains.
Ngày nay, việc sử dụng các chỉ thị phân tử ADN về đa hình các đoạn ADN nhân ngẫu nhiên-RAPD (Random Amplified Polymorphic DNA), đa hình độ dài các đoạn cắt giới hạn-RFLP (Restriction Fragment Length Polymorphism), sự lặp lại các chuỗi đơn giản-SSR (Simple Sequence Repeats) hay còn gọi là tiểu vệ tinh (Microsatellite) để phân loại, nghiên cứu đa dạng sinh học của động vật...
In routine histopathology, most tissues are fixed in formalin and embedded
in paraffin for long-term preservation. DNA can be extracted from these
tissues for subsequent molecular analysis by amplification methods. We
describe herein a protocol for DNA preparation from paraffin-embedded
tissues based on published procedures (1–3). In brief, tissue sections are placed
into microfuge tubes, then deparaffinized with xylene. The xylene is removed
with ethanol washes, and the tissue is treated with proteinase K to make DNA
available for amplification....
Linear Prediction Modelling of Speech Linear predictive models are widely used in speech processing applications such as low–bit–rate speech coding in cellular telephony, speech enhancement and speech recognition. Speech is generated by inhaling air into the lungs, and then exhaling it through the vibrating glottis cords and the vocal tract. The random, noise-like, air flow from the lungs is spectrally shaped and amplified by the vibrations of the glottal cords and the resonance of the vocal tract.
Inside SCRX2BC the signal is amplified and filtered in two stages between pins 14, 15, 16, 1, and 3. Pin 3 (DI)
is the output pulse sequence that was picked up by the receiver; this is used as the input to the decoder. The
SCRX2BC scans for the 4 long (synchronization) pulses and then counts the number of short pulses after them
to determine which command was sent by the transmitter. The gain of the SCRX2BC stages is high enough to
produce a pulse sequence at pin 3 even if no signal from a transmitter is present (it amplifies random noise),
but the resulting sequence...