Xem 1-20 trên 46 kết quả Resonance analysis
  • A new baculovirus-based fluorescence resonance energy transfer (Bv-FRET) assay for measuringmultimerization of cell surface molecules in living cells is described. It has been demonstrated that gonadotropin-releasing hormone recep-tor (GnRH-R) was capable of forming oligomeric com-plexes in the plasma membrane under normal physiological conditions. The mouse gonadotropin-releasing hormone receptor GnRH-R was used to evaluate the efficiency and potential applications of this assay.

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  • A library of random peptide sequences was used to select peptides that inhibit an anti-idiotypic catalytic Ig, immuno-globulin (IgG) 9G4H9, withab-lactamase-like activity. This library displays cyclic heptapeptides on the surface of bac-teriophagesand representsacollectionofupto4.5·10 9 peptides. The first selection step aimed at enriching the lib-rary in species that bind to the whole Ig molecule. The sec-ond stepwas todiscriminate peptides that bind topart of the molecule other than the active site. Selected peptides were then screened by surface plasmon resonance analysis.

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  • The tumor suppressor von Hippel–Lindau (VHL) gene product forms a com-plex with elongin B and elongin C, and acts as a recognition subunit of a ubiquitin E3 ligase. Interactions between components in the complex were investigated in living cells by fluorescence resonance energy transfer (FRET)–fluorescence lifetime imaging microscopy (FLIM).

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  • Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides.The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures.In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e.

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  • These days, it is impossible to think of an area of knowledge that can keep on developing without a collaboration in interdisciplinary fields. Electromotive force is a type of energy per unit of electric charge that is converted reversibly from chemical, mechanical, or other forms of energy into electrical energy by a dynamo, battery, electrochemical cells, thermoelectric devices, solar cells, transformers, and so on. The theory of electromotive Force is currently one of the most important research topics within the electrical engineering domain.

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  • The growth of magnetic resonance imaging (MRI) as a tool for studying brain function, as opposed to its more traditional role as a tool for studying brain anatomy and pathology, has been quite remarkable over the past decade. This has been driven in large measure by an appreciation of the considerable potential for functional magnetic resonance imaging (fMRI) to increase our understanding of how the human brain works, both in the normal and diseased states.

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  • Because of the availability of powerful computational techniques, new modality techniques such as Computer-Aided Tomography (CAT), Magnetic Resonance Imaging (MRI) and others, and because of the new techniques of imaging processing (machine vision), the lives of many patients will be saved, and the quality of all our lives improved. This marriage of powerful computer technology and medical imaging has spawned a new and growing generation of young dynamic doctors who hold PhDs in physics and/or computer science, along with their MDs.

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  • This book collects chapters on different theoretical and experimental aspects of photonics crystals for Nanophotonics applications. It is divided in two parts - a theoretical section and an experimental and applicative section.

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  • The last quarter of the last century has witnessed major advancements that have brought imaging and radioanalytical techniques to a paramount status in life sciences and industry. Generally speaking, the scope of radiation imaging and radioanalytics covers data acquisi‐ tion, data processing, and data analysis, involving theories, methods, systems and applica‐ tions. While detection and post-processing techniques become increasingly sophisticated, traditional and emerging modalities play more and more critical roles in medical and indus‐ trial domains.

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  • We utilized plasmon-waveguide resonance (PWR) spectroscopy to follow the effects of sphingomyelin, cholesterol and zinc ions on the binding and aggregation of the amyloidbpeptide1)40 in lipid bilayers. With a dioleoyl-phosphatidylcholine (DOPC) bilayer, peptide binding was observed, but no aggregation occurred over a period of 15 h.

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  • Neuronal PAS domain protein 2 (NPAS2) is a circadian rhythm-associated transcription factor with two heme-binding sites on two PAS domains. In the present study, we compared the optical absorption spectra, resonance Raman spectra, heme-binding kinetics and DNA-binding characteristics of the isolated fragment containing the N-terminal basic helix–loop–helix (bHLH) of the first PAS (PAS-A) domain of NPAS2 with those of the PAS-A domain alone.

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  • Quercetin 2,3-dioxygenase (2,3QD) is a copper-containing dioxygenase that catalyses the oxidation of the flavonol quercetin to 2-protocatechuoylphloroglucinol carboxylic acid with concomitant production of carbon monoxide. In contrast to iron dioxygenases, very little is known about copper dioxygenases. We have characterized 2,3QD from the fungus Aspergillus japonicus by electron paramagnetic resonance spectroscopy (EPR). At pH 6.0, 2,3QD shows a mixture of two EPR species. The major form has parameters typical of type 2 Cu sites (g// ¼ 2.330, A// ¼ 13.

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  • Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Research Article Independent Component Analysis for Magnetic Resonance Image Analysis

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  • The design and analysis of a racetrack resonator based on a multimode interference (MMI) coupler are presented in this paper. In order to describe the characteristics of an MMI coupler, a matrix description of the MMI coupler, which takes into account the effect of higher order modes in the structure, is developed. A design approach that is based on this matrix description is proposed. The usefulness of this design method is illustrated by means of an example based on Silicon on Insulator (SOI) technology....

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  • Therapists (allied health professionals and psychotherapists alike) are increasingly called upon to do research. Many are drawn to phenomenology; its holistic appreciation of everyday human experience resonates for them. Yet, as novice researcher-practitioners engage the field they are frequently brought up short, baffled by the language and sheer depth of ideas in this strange new world. Soon the novice is faced with bewildering choices.

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  • The solution structure of a recombinant mutant [rSP-C (FFI)] of the human surfactant-associatedprotein C (hSP-C) in a mixture of chloroform andmethanol was determined by high-resolution NMR spectroscopy. rSP-C (FFI) contains a helix from Phe5 to theC-terminal Leu34 andis thus longer by two residues than the helix of porcine SP-C (pSP-C), which is reportedto start at Val7 in the same solvent. Two sets of resonances at the C-terminus of the peptide were observed, which are explained by low-order oligomerization, probably dimerization of rSP-C (FFI) in its a-helical form. ...

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  • Different pathways of bilayer disruption by the structurally related antimicrobial peptides cecropin B, B1 and B3, revealed by surface plasma resonance analysis of immobi-lized liposomes, differential scanning calorimetryof peptide– large unilamellar vesicle interactions, and light microscopic analysis of peptide-treated giant unilamellar vesicles, have been identified inthis study.

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  • The solution structure of a synthetic mutant type I antifreeze protein (AFP I) was determined in aqueous solution at pH 7.0 using nuclear magnetic resonance (NMR) spectroscopy. The mutations comprised the replacement of the four Thr residues by Val and the introduction of two additional Lys-Glu salt bridges. The antifreeze activity of this mutant peptide, VVVV2KE, has been previously shown to be similar to that of the wild type protein, HPLC6 (defined here as TTTT).

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  • Guanosine triphosphate nucleotide analogues such as GppNHp (also named GMPPNP) or GTPcS are widely used to stabilize rapidly hydrolyzing protein-nucleotide complexes and to investigate biochemical reaction pathways. Here we describe the chemical synthesis of guanosine 5¢-O-(c-amidotriphosphate) (GTPcNH2) and a new synthesis of guanosine 5¢-O-(c-fluorotriphosphate) (GTPcF). The two nucleotides were characterized using NMR spectroscopy and isothermal titration calorimetry. Chemical shift data on 31P, 19F and 1H NMR resonances are tabulated.

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  • Modelling and computations in electromagnetics is a quite fast-growing research area. The recent interest in this field is caused by the increased demand for designing complex microwave components, modeling electromagnetic materials, and rapid increase in computational power for calculation of complex electromagnetic problems. The first part of this book is devoted to the advances in the analysis techniques such as method of moments, finite-difference time- domain method, boundary perturbation theory, Fourier analysis, mode-matching method, and analysis based on circuit theory....

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