A new baculovirus-based fluorescence resonance energy
transfer (Bv-FRET) assay for measuringmultimerization of
cell surface molecules in living cells is described. It has been
demonstrated that gonadotropin-releasing hormone recep-tor (GnRH-R) was capable of forming oligomeric com-plexes in the plasma membrane under normal physiological
conditions. The mouse gonadotropin-releasing hormone
receptor GnRH-R was used to evaluate the efficiency and
potential applications of this assay.
A library of random peptide sequences was used to select
peptides that inhibit an anti-idiotypic catalytic Ig, immuno-globulin (IgG) 9G4H9, withab-lactamase-like activity. This
library displays cyclic heptapeptides on the surface of bac-teriophagesand representsacollectionofupto4.5·10
peptides. The first selection step aimed at enriching the lib-rary in species that bind to the whole Ig molecule. The sec-ond stepwas todiscriminate peptides that bind topart of the
molecule other than the active site. Selected peptides were
then screened by surface plasmon resonance analysis.
The tumor suppressor von Hippel–Lindau (VHL) gene product forms a com-plex with elongin B and elongin C, and acts as a recognition subunit of a
ubiquitin E3 ligase. Interactions between components in the complex were
investigated in living cells by fluorescence resonance energy transfer
(FRET)–fluorescence lifetime imaging microscopy (FLIM).
Liquid chromatography mass spectrometry (LC-MS) is a
valuable tool in the analysis of proteins and peptides.The
combination of LC-MS with different fragmentation
methods provides sequence information on components in
complex mixtures.In this work, on-line packed capillary
LC electrospray ionization Fourier transform ion cyclotron
resonance MS was combined with two complementary
fragmentation techniques, i.e.
These days, it is impossible to think of an area of knowledge that can keep on
developing without a collaboration in interdisciplinary fields.
Electromotive force is a type of energy per unit of electric charge that is converted
reversibly from chemical, mechanical, or other forms of energy into electrical energy
by a dynamo, battery, electrochemical cells, thermoelectric devices, solar cells,
transformers, and so on.
The theory of electromotive Force is currently one of the most important research
topics within the electrical engineering domain.
The growth of magnetic resonance imaging (MRI) as a tool for studying brain function, as opposed
to its more traditional role as a tool for studying brain anatomy and pathology, has been quite
remarkable over the past decade. This has been driven in large measure by an appreciation of the
considerable potential for functional magnetic resonance imaging (fMRI) to increase our understanding
of how the human brain works, both in the normal and diseased states.
Because of the availability of powerful computational techniques, new modality
techniques such as Computer-Aided Tomography (CAT), Magnetic Resonance
Imaging (MRI) and others, and because of the new techniques of imaging processing
(machine vision), the lives of many patients will be saved, and the quality of
all our lives improved. This marriage of powerful computer technology and medical
imaging has spawned a new and growing generation of young dynamic doctors
who hold PhDs in physics and/or computer science, along with their MDs.
This book collects chapters on different theoretical and experimental aspects of photonics crystals for Nanophotonics applications. It is divided in two parts - a theoretical section and an experimental and applicative section.
The last quarter of the last century has witnessed major advancements that have brought
imaging and radioanalytical techniques to a paramount status in life sciences and industry.
Generally speaking, the scope of radiation imaging and radioanalytics covers data acquisi‐
tion, data processing, and data analysis, involving theories, methods, systems and applica‐
tions. While detection and post-processing techniques become increasingly sophisticated,
traditional and emerging modalities play more and more critical roles in medical and indus‐
We utilized plasmon-waveguide resonance (PWR) spectroscopy to follow
the effects of sphingomyelin, cholesterol and zinc ions on the binding and
aggregation of the amyloidbpeptide1)40
in lipid bilayers. With a dioleoyl-phosphatidylcholine (DOPC) bilayer, peptide binding was observed, but no
aggregation occurred over a period of 15 h.
Neuronal PAS domain protein 2 (NPAS2) is a circadian rhythm-associated
transcription factor with two heme-binding sites on two PAS domains. In
the present study, we compared the optical absorption spectra, resonance
Raman spectra, heme-binding kinetics and DNA-binding characteristics of
the isolated fragment containing the N-terminal basic helix–loop–helix
(bHLH) of the first PAS (PAS-A) domain of NPAS2 with those of the
PAS-A domain alone.
Quercetin 2,3-dioxygenase (2,3QD) is a copper-containing dioxygenase that catalyses the oxidation of the ﬂavonol quercetin to 2-protocatechuoylphloroglucinol carboxylic acid with concomitant production of carbon monoxide. In contrast to iron dioxygenases, very little is known about copper dioxygenases. We have characterized 2,3QD from the fungus Aspergillus japonicus by electron paramagnetic resonance spectroscopy (EPR). At pH 6.0, 2,3QD shows a mixture of two EPR species. The major form has parameters typical of type 2 Cu sites (g// ¼ 2.330, A// ¼ 13.
The design and analysis of a racetrack resonator based on a multimode interference
(MMI) coupler are presented in this paper. In order to describe the characteristics of an MMI
coupler, a matrix description of the MMI coupler, which takes into account the effect of higher
order modes in the structure, is developed. A design approach that is based on this matrix
description is proposed. The usefulness of this design method is illustrated by means of an
example based on Silicon on Insulator (SOI) technology....
Therapists (allied health professionals and psychotherapists alike) are increasingly
called upon to do research. Many are drawn to phenomenology;
its holistic appreciation of everyday human experience resonates for them.
Yet, as novice researcher-practitioners engage the field they are frequently
brought up short, baffled by the language and sheer depth of ideas in this
strange new world. Soon the novice is faced with bewildering choices.
The solution structure of a recombinant mutant [rSP-C (FFI)] of the human surfactant-associatedprotein C
(hSP-C) in a mixture of chloroform andmethanol was
determined by high-resolution NMR spectroscopy. rSP-C (FFI) contains a helix from Phe5 to theC-terminal Leu34
andis thus longer by two residues than the helix of porcine
SP-C (pSP-C), which is reportedto start at Val7 in the same
solvent. Two sets of resonances at the C-terminus of the
peptide were observed, which are explained by low-order
oligomerization, probably dimerization of rSP-C (FFI) in
its a-helical form. ...
Different pathways of bilayer disruption by the structurally
related antimicrobial peptides cecropin B, B1 and B3,
revealed by surface plasma resonance analysis of immobi-lized liposomes, differential scanning calorimetryof peptide–
large unilamellar vesicle interactions, and light microscopic
analysis of peptide-treated giant unilamellar vesicles, have
been identified inthis study.
The solution structure of a synthetic mutant type I antifreeze protein (AFP I) was determined in aqueous solution at pH 7.0 using nuclear magnetic resonance (NMR) spectroscopy. The mutations comprised the replacement of the four Thr residues by Val and the introduction of two additional Lys-Glu salt bridges. The antifreeze activity of this mutant peptide, VVVV2KE, has been previously shown to be similar to that of the wild type protein, HPLC6 (deﬁned here as TTTT).
Guanosine triphosphate nucleotide analogues such as GppNHp (also named GMPPNP) or GTPcS are widely used to stabilize rapidly hydrolyzing protein-nucleotide complexes and to investigate biochemical reaction pathways. Here we describe the chemical synthesis of guanosine 5¢-O-(c-amidotriphosphate) (GTPcNH2) and a new synthesis of guanosine 5¢-O-(c-ﬂuorotriphosphate) (GTPcF). The two nucleotides were characterized using NMR spectroscopy and isothermal titration calorimetry. Chemical shift data on 31P, 19F and 1H NMR resonances are tabulated.
Modelling and computations in electromagnetics is a quite fast-growing research area. The recent interest in this field is caused by the increased demand for designing complex microwave components, modeling electromagnetic materials, and rapid increase in computational power for calculation of complex electromagnetic problems. The first part of this book is devoted to the advances in the analysis techniques such as method of moments, finite-difference time- domain method, boundary perturbation theory, Fourier analysis, mode-matching method, and analysis based on circuit theory....