Xem 1-5 trên 5 kết quả Ribonuclease cytosolic
  • Rv3619c and Rv3620c are the secretory, antigenic proteins of the ESAT-6⁄CFP-10 family ofMycobacterium tuberculosisH37Rv. In this article, we show that Rv3619c interacts with Rv3620c to form a 1 : 1 heterodimeric complex with a dissociation constant (Kd) of 4.8·10 )7 M. The thermal unfolding of the heterodimer was completely reversible, with a Tm of 48C.

    pdf10p mobifone23 07-01-2013 11 3   Download

  • The cytotoxic action of some ribonucleases homologous to bovine pancre-atic RNase A, the superfamily prototype, has interested and intrigued investigators. Their ribonucleolytic activity is essential for their cytotoxic action, and their target RNA is in the cytosol. It has been proposed that the cytosolic RNase inhibitor (cRI) plays a major role in determining the ability of an RNase to be cytotoxic.

    pdf11p inspiron33 25-03-2013 11 3   Download

  • Onconase, a member of the ribonuclease superfamily, is a potent cytotoxicagent that isundergoingphase II/IIIhuman clinical trials as an antitumor drug. Native onconase from Rana pipiensand its amphibian homologs have an N-ter-minal pyroglutamyl residue that is essential for obtaining fully active enzymes with their full potential as cytotoxins. When expressed cytosolically in bacteria, Onconase is isolated with an additional methionyl (Met1) residue and glutaminyl insteadof a pyroglutamyl residue at position 1 of the N-terminus and is consequently inactivated....

    pdf9p dell39 03-04-2013 11 2   Download

  • Onconase  (ONC) is a member of the ribonuclease A superfamily that is toxic to cancer cells in vitro andin vivo. ONC is now in Phase IIIb clinical trials for the treatment of malignant mesothelioma. Internalization of ONC to the cytosol of cancer cells is essential for its cytotoxic activity, despite the apparent absence of a cell-surface receptor protein.

    pdf12p vinaphone15 25-02-2013 6 1   Download

  • The dimeric structure of seminal ribonuclease (BS-RNase) is maintained by noncovalent interactions and by two intersubunit disulfide bridges.Another unusual feature of this enzyme is its antitumour action, consisting in a cyto-toxic activity selective for malignant cells.This cytotoxic action is exerted when the protein reaches the cytosol of the affected cells, where it degrades ribosomal RNA, thus blocking protein synthesis and leading cells to death.

    pdf8p fptmusic 16-04-2013 10 1   Download

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